The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model
Vibrio parahaemolyticus, a marine bacterium, is the causative agent of gastroenteritis associated with the consumption of seafood. It contains a homologue of the toxRS operon that in V. cholerae is the key regulator of virulence gene expression. We examined a nonpolar mutation in toxRS to determine...
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American Society for Microbiology
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Online Access: | http://hdl.handle.net/10379/3855 https://doi.org/10.1128/IAI.06284-11 |
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ftnuigalway:oai:aran.library.nuigalway.ie/:10379/3855 2023-06-11T04:11:11+02:00 The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model Boyd, Aoife |~| 2013-09-20T14:12:26Z application/pdf http://hdl.handle.net/10379/3855 https://doi.org/10.1128/IAI.06284-11 en eng American Society for Microbiology Infection And Immunity Whitaker, WB,Parent, MA,Boyd, A,Richards, GP,Boyd, EF (2012) 'The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model'. Infection And Immunity, 80 :1834-1845. http://hdl.handle.net/10379/3855 doi:10.1128/IAI.06284-11 Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ THERMOSTABLE DIRECT HEMOLYSIN III SECRETION SYSTEM CHOLERA-TOXIN INTESTINAL COLONIZATION ANTIMICROBIAL PEPTIDES CRASSOSTREA-GIGAS VIRULENCE FACTORS ACCESSORY TOXINS CLINICAL ISOLATE EFFECTOR PROTEIN Article 2013 ftnuigalway https://doi.org/10.1128/IAI.06284-11 2023-05-28T18:03:43Z Vibrio parahaemolyticus, a marine bacterium, is the causative agent of gastroenteritis associated with the consumption of seafood. It contains a homologue of the toxRS operon that in V. cholerae is the key regulator of virulence gene expression. We examined a nonpolar mutation in toxRS to determine the role of these genes in V. parahaemolyticus RIMD2210633, an O3:K6 isolate, and showed that compared to the wild type, Delta toxRS was significantly more sensitive to acid, bile salts, and sodium dodecyl sulfate stresses. We demonstrated that ToxRS is a positive regulator of ompU expression, and that the complementation of Delta toxRS with ompU restores stress tolerance. Furthermore, we showed that ToxRS also regulates type III secretion system genes in chromosome I via the regulation of the leuO homologue VP0350. We examined the effect of Delta toxRS in vivo using a new orogastric adult murine model of colonization. We demonstrated that streptomycin-treated adult C57BL/6 mice experienced prolonged intestinal colonization along the entire intestinal tract by the streptomycin-resistant V. parahaemolyticus. In contrast, no colonization occurred in non-streptomycin-treated mice. A competition assay between the Delta toxRS and wild-type V. parahaemolyticus strains marked with the beta-galactosidase gene lacZ demonstrated that the Delta toxRS strain was defective in colonization compared to the wild-type strain. This defect was rescued by ectopically expressing ompU. Thus, the defect in stress tolerance and colonization in Delta toxRS is solely due to OmpU. To our knowledge, the orogastric adult murine model reported here is the first showing sustained intestinal colonization by V. parahaemolyticus. peer-reviewed Article in Journal/Newspaper Crassostrea gigas National University of Ireland (NUI), Galway: ARAN Infection and Immunity 80 5 1834 1845 |
institution |
Open Polar |
collection |
National University of Ireland (NUI), Galway: ARAN |
op_collection_id |
ftnuigalway |
language |
English |
topic |
THERMOSTABLE DIRECT HEMOLYSIN III SECRETION SYSTEM CHOLERA-TOXIN INTESTINAL COLONIZATION ANTIMICROBIAL PEPTIDES CRASSOSTREA-GIGAS VIRULENCE FACTORS ACCESSORY TOXINS CLINICAL ISOLATE EFFECTOR PROTEIN |
spellingShingle |
THERMOSTABLE DIRECT HEMOLYSIN III SECRETION SYSTEM CHOLERA-TOXIN INTESTINAL COLONIZATION ANTIMICROBIAL PEPTIDES CRASSOSTREA-GIGAS VIRULENCE FACTORS ACCESSORY TOXINS CLINICAL ISOLATE EFFECTOR PROTEIN Boyd, Aoife The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model |
topic_facet |
THERMOSTABLE DIRECT HEMOLYSIN III SECRETION SYSTEM CHOLERA-TOXIN INTESTINAL COLONIZATION ANTIMICROBIAL PEPTIDES CRASSOSTREA-GIGAS VIRULENCE FACTORS ACCESSORY TOXINS CLINICAL ISOLATE EFFECTOR PROTEIN |
description |
Vibrio parahaemolyticus, a marine bacterium, is the causative agent of gastroenteritis associated with the consumption of seafood. It contains a homologue of the toxRS operon that in V. cholerae is the key regulator of virulence gene expression. We examined a nonpolar mutation in toxRS to determine the role of these genes in V. parahaemolyticus RIMD2210633, an O3:K6 isolate, and showed that compared to the wild type, Delta toxRS was significantly more sensitive to acid, bile salts, and sodium dodecyl sulfate stresses. We demonstrated that ToxRS is a positive regulator of ompU expression, and that the complementation of Delta toxRS with ompU restores stress tolerance. Furthermore, we showed that ToxRS also regulates type III secretion system genes in chromosome I via the regulation of the leuO homologue VP0350. We examined the effect of Delta toxRS in vivo using a new orogastric adult murine model of colonization. We demonstrated that streptomycin-treated adult C57BL/6 mice experienced prolonged intestinal colonization along the entire intestinal tract by the streptomycin-resistant V. parahaemolyticus. In contrast, no colonization occurred in non-streptomycin-treated mice. A competition assay between the Delta toxRS and wild-type V. parahaemolyticus strains marked with the beta-galactosidase gene lacZ demonstrated that the Delta toxRS strain was defective in colonization compared to the wild-type strain. This defect was rescued by ectopically expressing ompU. Thus, the defect in stress tolerance and colonization in Delta toxRS is solely due to OmpU. To our knowledge, the orogastric adult murine model reported here is the first showing sustained intestinal colonization by V. parahaemolyticus. peer-reviewed |
author2 |
|~| |
format |
Article in Journal/Newspaper |
author |
Boyd, Aoife |
author_facet |
Boyd, Aoife |
author_sort |
Boyd, Aoife |
title |
The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model |
title_short |
The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model |
title_full |
The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model |
title_fullStr |
The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model |
title_full_unstemmed |
The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model |
title_sort |
vibrio parahaemolyticus toxrs regulator is required for stress tolerance and colonization in a novel orogastric streptomycin-induced adult murine model |
publisher |
American Society for Microbiology |
publishDate |
2013 |
url |
http://hdl.handle.net/10379/3855 https://doi.org/10.1128/IAI.06284-11 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
Infection And Immunity Whitaker, WB,Parent, MA,Boyd, A,Richards, GP,Boyd, EF (2012) 'The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model'. Infection And Immunity, 80 :1834-1845. http://hdl.handle.net/10379/3855 doi:10.1128/IAI.06284-11 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ |
op_doi |
https://doi.org/10.1128/IAI.06284-11 |
container_title |
Infection and Immunity |
container_volume |
80 |
container_issue |
5 |
container_start_page |
1834 |
op_container_end_page |
1845 |
_version_ |
1768386087391068160 |