Vivaspin ultrafiltration: a new approach for high resolution measurements of colloidal and soluble iron species
Vivaspin6 (R) ultrafiltration units with molecular weight "cut-off" membranes of 5, 10, 30, 50, and 100 kDa were used together to examine the size distribution of newly formed iron (Fe) colloids in natural seawater samples and in the presence of several different Fe chelators with varying...
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Online Access: | http://hdl.handle.net/10379/13821 https://doi.org/10.4319/lom.2013.11.187 |
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ftnuigalway:oai:aran.library.nuigalway.ie/:10379/13821 2023-06-11T04:15:44+02:00 Vivaspin ultrafiltration: a new approach for high resolution measurements of colloidal and soluble iron species Schlosser, Christian Streu, Peter Croot, Peter L. 2013-04-01 http://hdl.handle.net/10379/13821 https://doi.org/10.4319/lom.2013.11.187 unknown Wiley-Blackwell Limnology and Oceanography: Methods Schlosser, Christian; Streu, Peter; Croot, Peter L. (2013). Vivaspin ultrafiltration: a new approach for high resolution measurements of colloidal and soluble iron species. Limnology and Oceanography: Methods 11 , 187-201 1541-5856 http://hdl.handle.net/10379/13821 doi:10.4319/lom.2013.11.187 Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ fluorescent organic-matter north pacific-ocean iron(iii) hydroxide solubility cross-flow ultrafiltration southern-ocean vertical-distribution solvent-extraction water column okhotsk sea seawater Article 2013 ftnuigalway https://doi.org/10.4319/lom.2013.11.187 2023-05-28T18:05:56Z Vivaspin6 (R) ultrafiltration units with molecular weight "cut-off" membranes of 5, 10, 30, 50, and 100 kDa were used together to examine the size distribution of newly formed iron (Fe) colloids in natural seawater samples and in the presence of several different Fe chelators with varying Fe binding strength. Artificial Fe chelators, such as TAC, and 2 kDG, when added at equimolar levels to Fe, supported the formation of a continuum of Fe-ligand colloids between 5 and 100 kDa. More than 90% of the added Fe-55 in these solutions occurred in Fe aggregates/particles larger than 100 kDa. The strong siderophore DFO held the majority of the added Fe-55 in the "truly" soluble fraction <= 5 kDa, whereas 90% of Fe-55 added to UV-irradiated seawater was converted into Fe colloids with a size between 50 to 100 kDa (5-6 nm). Membranes with >= 10 kDa showed similar "cut-off" properties on natural seawater samples collected in the water column off the Peruvian coast. Fe solubility determined with these membranes was approximately six times greater than Fe solubility determined with the 5 kDa membrane and the 0.02 mu m syringe filters. This suggests that a seamless size continuum of organic chelators (<= 5 kDa-10 kDa) is present in these seawaters and that estimates of ligand production based on 0.02 mu m Anotop solubility experiments underestimates the abundance of soluble/colloidal ligands. Regarding these results, we recommend the use of Vivaspin 5 kDa membranes to separate the "truly" soluble from the colloidal Fe fraction. Article in Journal/Newspaper okhotsk sea Southern Ocean National University of Ireland (NUI), Galway: ARAN Southern Ocean Okhotsk Pacific Tac ENVELOPE(-59.517,-59.517,-62.500,-62.500) Limnology and Oceanography: Methods 11 4 187 201 |
institution |
Open Polar |
collection |
National University of Ireland (NUI), Galway: ARAN |
op_collection_id |
ftnuigalway |
language |
unknown |
topic |
fluorescent organic-matter north pacific-ocean iron(iii) hydroxide solubility cross-flow ultrafiltration southern-ocean vertical-distribution solvent-extraction water column okhotsk sea seawater |
spellingShingle |
fluorescent organic-matter north pacific-ocean iron(iii) hydroxide solubility cross-flow ultrafiltration southern-ocean vertical-distribution solvent-extraction water column okhotsk sea seawater Schlosser, Christian Streu, Peter Croot, Peter L. Vivaspin ultrafiltration: a new approach for high resolution measurements of colloidal and soluble iron species |
topic_facet |
fluorescent organic-matter north pacific-ocean iron(iii) hydroxide solubility cross-flow ultrafiltration southern-ocean vertical-distribution solvent-extraction water column okhotsk sea seawater |
description |
Vivaspin6 (R) ultrafiltration units with molecular weight "cut-off" membranes of 5, 10, 30, 50, and 100 kDa were used together to examine the size distribution of newly formed iron (Fe) colloids in natural seawater samples and in the presence of several different Fe chelators with varying Fe binding strength. Artificial Fe chelators, such as TAC, and 2 kDG, when added at equimolar levels to Fe, supported the formation of a continuum of Fe-ligand colloids between 5 and 100 kDa. More than 90% of the added Fe-55 in these solutions occurred in Fe aggregates/particles larger than 100 kDa. The strong siderophore DFO held the majority of the added Fe-55 in the "truly" soluble fraction <= 5 kDa, whereas 90% of Fe-55 added to UV-irradiated seawater was converted into Fe colloids with a size between 50 to 100 kDa (5-6 nm). Membranes with >= 10 kDa showed similar "cut-off" properties on natural seawater samples collected in the water column off the Peruvian coast. Fe solubility determined with these membranes was approximately six times greater than Fe solubility determined with the 5 kDa membrane and the 0.02 mu m syringe filters. This suggests that a seamless size continuum of organic chelators (<= 5 kDa-10 kDa) is present in these seawaters and that estimates of ligand production based on 0.02 mu m Anotop solubility experiments underestimates the abundance of soluble/colloidal ligands. Regarding these results, we recommend the use of Vivaspin 5 kDa membranes to separate the "truly" soluble from the colloidal Fe fraction. |
format |
Article in Journal/Newspaper |
author |
Schlosser, Christian Streu, Peter Croot, Peter L. |
author_facet |
Schlosser, Christian Streu, Peter Croot, Peter L. |
author_sort |
Schlosser, Christian |
title |
Vivaspin ultrafiltration: a new approach for high resolution measurements of colloidal and soluble iron species |
title_short |
Vivaspin ultrafiltration: a new approach for high resolution measurements of colloidal and soluble iron species |
title_full |
Vivaspin ultrafiltration: a new approach for high resolution measurements of colloidal and soluble iron species |
title_fullStr |
Vivaspin ultrafiltration: a new approach for high resolution measurements of colloidal and soluble iron species |
title_full_unstemmed |
Vivaspin ultrafiltration: a new approach for high resolution measurements of colloidal and soluble iron species |
title_sort |
vivaspin ultrafiltration: a new approach for high resolution measurements of colloidal and soluble iron species |
publisher |
Wiley-Blackwell |
publishDate |
2013 |
url |
http://hdl.handle.net/10379/13821 https://doi.org/10.4319/lom.2013.11.187 |
long_lat |
ENVELOPE(-59.517,-59.517,-62.500,-62.500) |
geographic |
Southern Ocean Okhotsk Pacific Tac |
geographic_facet |
Southern Ocean Okhotsk Pacific Tac |
genre |
okhotsk sea Southern Ocean |
genre_facet |
okhotsk sea Southern Ocean |
op_relation |
Limnology and Oceanography: Methods Schlosser, Christian; Streu, Peter; Croot, Peter L. (2013). Vivaspin ultrafiltration: a new approach for high resolution measurements of colloidal and soluble iron species. Limnology and Oceanography: Methods 11 , 187-201 1541-5856 http://hdl.handle.net/10379/13821 doi:10.4319/lom.2013.11.187 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ |
op_doi |
https://doi.org/10.4319/lom.2013.11.187 |
container_title |
Limnology and Oceanography: Methods |
container_volume |
11 |
container_issue |
4 |
container_start_page |
187 |
op_container_end_page |
201 |
_version_ |
1768372808605237248 |