Saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth inulva rigida(chlorophyta)
Carbon physiology of a genetically identified Ulva rigida was investigated under different CO2(aq) and light levels. The study was designed to answer whether (1) light or exogenous inorganic carbon (Ci) pool is driving growth; and (2) elevated CO2(aq) concentration under ocean acidification (OA) wil...
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2015
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Online Access: | http://hdl.handle.net/10379/13602 https://doi.org/10.1002/ece3.1382 |
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ftnuigalway:oai:aran.library.nuigalway.ie/:10379/13602 2023-06-11T04:15:37+02:00 Saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth inulva rigida(chlorophyta) Rautenberger, Ralf Fernández, Pamela A. Strittmatter, Martina Heesch, Svenja Cornwall, Christopher E. Hurd, Catriona L. Roleda, Michael Y. 2015-01-25 http://hdl.handle.net/10379/13602 https://doi.org/10.1002/ece3.1382 unknown Wiley-Blackwell Ecology and Evolution Rautenberger, Ralf; Fernández, Pamela A. Strittmatter, Martina; Heesch, Svenja; Cornwall, Christopher E.; Hurd, Catriona L.; Roleda, Michael Y. (2015). Saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth inulva rigida(chlorophyta). Ecology and Evolution 5 (4), 874-888 2045-7758 http://hdl.handle.net/10379/13602 doi:10.1002/ece3.1382 Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ bicarbonate c:n ratio carbon physiology carbon-concentrating mechanism carbonic anhydrase chlorophyll fluorescence f-v/f-m pigments seaweed stable carbon isotope macrocystis-pyrifera laminariales artificial seawater medium anion-exchange protein green tide alga inorganic-carbon marine macroalgae bicarbonate uptake chlamydomonas-reinhardtii emiliania-huxleyi hco3-utilization Article 2015 ftnuigalway https://doi.org/10.1002/ece3.1382 2023-05-28T18:05:52Z Carbon physiology of a genetically identified Ulva rigida was investigated under different CO2(aq) and light levels. The study was designed to answer whether (1) light or exogenous inorganic carbon (Ci) pool is driving growth; and (2) elevated CO2(aq) concentration under ocean acidification (OA) will downregulate CA(ext)-mediated HCO3- dehydration and alter the stable carbon isotope (C-13) signatures toward more CO2 use to support higher growth rate. At pH(T) 9.0 where CO2(aq) is <1mol L-1, inhibition of the known HCO3- use mechanisms, that is, direct HCO3- uptake through the AE port and CA(ext)-mediated HCO3- dehydration decreased net photosynthesis (NPS) by only 56-83%, leaving the carbon uptake mechanism for the remaining 17-44% of the NPS unaccounted. An in silico search for carbon-concentrating mechanism elements in expressed sequence tag libraries of Ulva found putative light-dependent HCO3- transporters to which the remaining NPS can be attributed. The shift in C-13 signatures from -22 parts per thousand toward -10 parts per thousand under saturating light but not under elevated CO2(aq) suggest preference and substantial HCO3- use to support photosynthesis and growth. U.rigida is Ci saturated, and growth was primarily controlled by light. Therefore, increased levels of CO2(aq) predicted for the future will not, in isolation, stimulate Ulva blooms. Article in Journal/Newspaper Ocean acidification National University of Ireland (NUI), Galway: ARAN Ecology and Evolution 5 4 874 888 |
institution |
Open Polar |
collection |
National University of Ireland (NUI), Galway: ARAN |
op_collection_id |
ftnuigalway |
language |
unknown |
topic |
bicarbonate c:n ratio carbon physiology carbon-concentrating mechanism carbonic anhydrase chlorophyll fluorescence f-v/f-m pigments seaweed stable carbon isotope macrocystis-pyrifera laminariales artificial seawater medium anion-exchange protein green tide alga inorganic-carbon marine macroalgae bicarbonate uptake chlamydomonas-reinhardtii emiliania-huxleyi hco3-utilization |
spellingShingle |
bicarbonate c:n ratio carbon physiology carbon-concentrating mechanism carbonic anhydrase chlorophyll fluorescence f-v/f-m pigments seaweed stable carbon isotope macrocystis-pyrifera laminariales artificial seawater medium anion-exchange protein green tide alga inorganic-carbon marine macroalgae bicarbonate uptake chlamydomonas-reinhardtii emiliania-huxleyi hco3-utilization Rautenberger, Ralf Fernández, Pamela A. Strittmatter, Martina Heesch, Svenja Cornwall, Christopher E. Hurd, Catriona L. Roleda, Michael Y. Saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth inulva rigida(chlorophyta) |
topic_facet |
bicarbonate c:n ratio carbon physiology carbon-concentrating mechanism carbonic anhydrase chlorophyll fluorescence f-v/f-m pigments seaweed stable carbon isotope macrocystis-pyrifera laminariales artificial seawater medium anion-exchange protein green tide alga inorganic-carbon marine macroalgae bicarbonate uptake chlamydomonas-reinhardtii emiliania-huxleyi hco3-utilization |
description |
Carbon physiology of a genetically identified Ulva rigida was investigated under different CO2(aq) and light levels. The study was designed to answer whether (1) light or exogenous inorganic carbon (Ci) pool is driving growth; and (2) elevated CO2(aq) concentration under ocean acidification (OA) will downregulate CA(ext)-mediated HCO3- dehydration and alter the stable carbon isotope (C-13) signatures toward more CO2 use to support higher growth rate. At pH(T) 9.0 where CO2(aq) is <1mol L-1, inhibition of the known HCO3- use mechanisms, that is, direct HCO3- uptake through the AE port and CA(ext)-mediated HCO3- dehydration decreased net photosynthesis (NPS) by only 56-83%, leaving the carbon uptake mechanism for the remaining 17-44% of the NPS unaccounted. An in silico search for carbon-concentrating mechanism elements in expressed sequence tag libraries of Ulva found putative light-dependent HCO3- transporters to which the remaining NPS can be attributed. The shift in C-13 signatures from -22 parts per thousand toward -10 parts per thousand under saturating light but not under elevated CO2(aq) suggest preference and substantial HCO3- use to support photosynthesis and growth. U.rigida is Ci saturated, and growth was primarily controlled by light. Therefore, increased levels of CO2(aq) predicted for the future will not, in isolation, stimulate Ulva blooms. |
format |
Article in Journal/Newspaper |
author |
Rautenberger, Ralf Fernández, Pamela A. Strittmatter, Martina Heesch, Svenja Cornwall, Christopher E. Hurd, Catriona L. Roleda, Michael Y. |
author_facet |
Rautenberger, Ralf Fernández, Pamela A. Strittmatter, Martina Heesch, Svenja Cornwall, Christopher E. Hurd, Catriona L. Roleda, Michael Y. |
author_sort |
Rautenberger, Ralf |
title |
Saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth inulva rigida(chlorophyta) |
title_short |
Saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth inulva rigida(chlorophyta) |
title_full |
Saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth inulva rigida(chlorophyta) |
title_fullStr |
Saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth inulva rigida(chlorophyta) |
title_full_unstemmed |
Saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth inulva rigida(chlorophyta) |
title_sort |
saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth inulva rigida(chlorophyta) |
publisher |
Wiley-Blackwell |
publishDate |
2015 |
url |
http://hdl.handle.net/10379/13602 https://doi.org/10.1002/ece3.1382 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Ecology and Evolution Rautenberger, Ralf; Fernández, Pamela A. Strittmatter, Martina; Heesch, Svenja; Cornwall, Christopher E.; Hurd, Catriona L.; Roleda, Michael Y. (2015). Saturating light and not increased carbon dioxide under ocean acidification drives photosynthesis and growth inulva rigida(chlorophyta). Ecology and Evolution 5 (4), 874-888 2045-7758 http://hdl.handle.net/10379/13602 doi:10.1002/ece3.1382 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ |
op_doi |
https://doi.org/10.1002/ece3.1382 |
container_title |
Ecology and Evolution |
container_volume |
5 |
container_issue |
4 |
container_start_page |
874 |
op_container_end_page |
888 |
_version_ |
1768372583036616704 |