Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin
European Community regulations on chemicals promote alternative methods to test substances presenting potential risks for the environment. In the present work, cultured atrial cells isolated from oyster (Crassostrea gigas) were used as an experimental model to investigate the toxicity of tributyltin...
Published in: | Aquatic Living Resources |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
EDP Sciences
2012
|
Subjects: | |
Online Access: | http://hdl.handle.net/10379/11258 https://doi.org/10.1051/alr/2012017 |
id |
ftnuigalway:oai:aran.library.nuigalway.ie/:10379/11258 |
---|---|
record_format |
openpolar |
spelling |
ftnuigalway:oai:aran.library.nuigalway.ie/:10379/11258 2023-06-11T04:11:07+02:00 Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin Droguet, Mickaël Devauchelle, Nicole Pennec, Jean-Pierre Quinn, Brian Dorange, Germaine 2012-04-01 http://hdl.handle.net/10379/11258 https://doi.org/10.1051/alr/2012017 unknown EDP Sciences Aquatic Living Resources Droguet, Mickaël; Devauchelle, Nicole; Pennec, Jean-Pierre; Quinn, Brian; Dorange, Germaine (2012). Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin. Aquatic Living Resources 25 (2), 185-194 0990-7440,1765-2952 http://hdl.handle.net/10379/11258 doi:10.1051/alr/2012017 Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ oyster heart cells flow cytometry tributyltin toxicity antifouling organotin compounds crassostrea-gigas induced apoptosis ruditapes-philippinarum antifouling compounds intracellular ca2+ neuronal cell mya-arenaria manila clam shell Article 2012 ftnuigalway https://doi.org/10.1051/alr/2012017 2023-05-28T18:05:15Z European Community regulations on chemicals promote alternative methods to test substances presenting potential risks for the environment. In the present work, cultured atrial cells isolated from oyster (Crassostrea gigas) were used as an experimental model to investigate the toxicity of tributyltin (TBT) after short-time exposure at concentrations representative of those that can be measured in seawater, marine sediments and/or bivalves bioaccumulating this pollutant. In vitro and in vivo assays produce values of the same order of magnitude for both animal/cell survival and heart/cardiomyocyte beating rate. The survival rate of whole animals decreased from 10(-6) M TBT after 3 days. For cultured cells, the viability, evaluated using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay, significantly decreased after two days of treatment with 10(-6) M TBT, and after six days with 10(-10) M TBT. The percentage of apoptotic cells, quantified by flow cytometry and YO-PRO (R)-1 iodide, a nucleic acid stain that only permeates cells that are beginning to undergo apoptosis, increased significantly in these cases. Moreover, intracellular concentration of Ca++ had increased after 10 min of exposition to 10(-6) M, and could be associated with apoptotic processes. As patch clamp experiments showed that Ca++ conductance was decreased, intracellular calcium increase could mainly be due to a release from internal stores. The decreases in beating rhythm could be explained by the decrease in adenosine triphosphate (ATP) production revealed by P-31 nuclear magnetic resonance (NMR) spectroscopy and confirmed by the increase of the K-ATP channel conductance. The related hyperpolarization and the disturbances of the energetic metabolism were clearly related to the loss of the atrial cell contractility and viability. Article in Journal/Newspaper Crassostrea gigas National University of Ireland (NUI), Galway: ARAN Aquatic Living Resources 25 2 185 194 |
institution |
Open Polar |
collection |
National University of Ireland (NUI), Galway: ARAN |
op_collection_id |
ftnuigalway |
language |
unknown |
topic |
oyster heart cells flow cytometry tributyltin toxicity antifouling organotin compounds crassostrea-gigas induced apoptosis ruditapes-philippinarum antifouling compounds intracellular ca2+ neuronal cell mya-arenaria manila clam shell |
spellingShingle |
oyster heart cells flow cytometry tributyltin toxicity antifouling organotin compounds crassostrea-gigas induced apoptosis ruditapes-philippinarum antifouling compounds intracellular ca2+ neuronal cell mya-arenaria manila clam shell Droguet, Mickaël Devauchelle, Nicole Pennec, Jean-Pierre Quinn, Brian Dorange, Germaine Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin |
topic_facet |
oyster heart cells flow cytometry tributyltin toxicity antifouling organotin compounds crassostrea-gigas induced apoptosis ruditapes-philippinarum antifouling compounds intracellular ca2+ neuronal cell mya-arenaria manila clam shell |
description |
European Community regulations on chemicals promote alternative methods to test substances presenting potential risks for the environment. In the present work, cultured atrial cells isolated from oyster (Crassostrea gigas) were used as an experimental model to investigate the toxicity of tributyltin (TBT) after short-time exposure at concentrations representative of those that can be measured in seawater, marine sediments and/or bivalves bioaccumulating this pollutant. In vitro and in vivo assays produce values of the same order of magnitude for both animal/cell survival and heart/cardiomyocyte beating rate. The survival rate of whole animals decreased from 10(-6) M TBT after 3 days. For cultured cells, the viability, evaluated using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay, significantly decreased after two days of treatment with 10(-6) M TBT, and after six days with 10(-10) M TBT. The percentage of apoptotic cells, quantified by flow cytometry and YO-PRO (R)-1 iodide, a nucleic acid stain that only permeates cells that are beginning to undergo apoptosis, increased significantly in these cases. Moreover, intracellular concentration of Ca++ had increased after 10 min of exposition to 10(-6) M, and could be associated with apoptotic processes. As patch clamp experiments showed that Ca++ conductance was decreased, intracellular calcium increase could mainly be due to a release from internal stores. The decreases in beating rhythm could be explained by the decrease in adenosine triphosphate (ATP) production revealed by P-31 nuclear magnetic resonance (NMR) spectroscopy and confirmed by the increase of the K-ATP channel conductance. The related hyperpolarization and the disturbances of the energetic metabolism were clearly related to the loss of the atrial cell contractility and viability. |
format |
Article in Journal/Newspaper |
author |
Droguet, Mickaël Devauchelle, Nicole Pennec, Jean-Pierre Quinn, Brian Dorange, Germaine |
author_facet |
Droguet, Mickaël Devauchelle, Nicole Pennec, Jean-Pierre Quinn, Brian Dorange, Germaine |
author_sort |
Droguet, Mickaël |
title |
Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin |
title_short |
Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin |
title_full |
Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin |
title_fullStr |
Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin |
title_full_unstemmed |
Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin |
title_sort |
cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin |
publisher |
EDP Sciences |
publishDate |
2012 |
url |
http://hdl.handle.net/10379/11258 https://doi.org/10.1051/alr/2012017 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
Aquatic Living Resources Droguet, Mickaël; Devauchelle, Nicole; Pennec, Jean-Pierre; Quinn, Brian; Dorange, Germaine (2012). Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin. Aquatic Living Resources 25 (2), 185-194 0990-7440,1765-2952 http://hdl.handle.net/10379/11258 doi:10.1051/alr/2012017 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ |
op_doi |
https://doi.org/10.1051/alr/2012017 |
container_title |
Aquatic Living Resources |
container_volume |
25 |
container_issue |
2 |
container_start_page |
185 |
op_container_end_page |
194 |
_version_ |
1768385989605064704 |