Comparison of assessment methods used to diagnose hematodinium sp. infections in cancer pagurus
Endoparasitic dinoflagellates of the genus Hematodinium have recently gained attention as significant pathogens of the brown crab Cancer pagurus in Ireland. Patent infections, which are characterized by a hyperpigmented carapace and moribund condition, are limited to the discrete periods when macros...
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Oxford University Press (OUP)
2011
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Online Access: | http://hdl.handle.net/10379/10783 https://doi.org/10.1093/icesjms/fsq197 |
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ftnuigalway:oai:aran.library.nuigalway.ie/:10379/10783 2023-06-11T04:10:57+02:00 Comparison of assessment methods used to diagnose hematodinium sp. infections in cancer pagurus Ni Chualain, C. Robinson, M. 2011-01-17 http://hdl.handle.net/10379/10783 https://doi.org/10.1093/icesjms/fsq197 unknown Oxford University Press (OUP) ICES Journal of Marine Science Ni Chualain, C. Robinson, M. (2011). Comparison of assessment methods used to diagnose hematodinium sp. infections in cancer pagurus. ICES Journal of Marine Science 68 (3), 454-462 1054-3139,1095-9289 http://hdl.handle.net/10379/10783 doi:10.1093/icesjms/fsq197 Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ cancer pagurus diagnosis hematodinium sp histology pcr lobster nephrops-norvegicus crabs chionoecetes-opilio parasitic dinoflagellate hematodinium callinectes-sapidus blue-crab molecular-detection conception bay disease newfoundland prevalence Article 2011 ftnuigalway https://doi.org/10.1093/icesjms/fsq197 2023-05-28T18:05:07Z Endoparasitic dinoflagellates of the genus Hematodinium have recently gained attention as significant pathogens of the brown crab Cancer pagurus in Ireland. Patent infections, which are characterized by a hyperpigmented carapace and moribund condition, are limited to the discrete periods when macroscopic identification is possible. Three methods are assessed for diagnosing Hematodinium sp. infections in brown crab at the times when macroscopic identification is not always possible. Haemolymph smears, histological sections of gill, heart, midgut, hepatopancreas, muscle, and gonad, and a polymerase chain reaction (PCR) assay provided virtually equivalent accuracy in gauging infection prevalence, regardless of season. Sequences of PCR amplicons from the 18S ribosomal RNA gene confirmed the identity of the parasite as belonging to the genus Hematodinium. Infection intensity values (< 1-87%) obtained from haemolymph smears underscored infection levels within tissues, 90% of which contained advanced levels of infection. Alterations to tissues of infected crabs included haemocytopoenia, oedema, which caused dilation of the haemal sinuses resulting in pressure necrosis to the connective tissues around the oocytes, myocardial bundles, and hepatopancreatic tubules. The claw muscle of infected animals contained the fewest parasites. Article in Journal/Newspaper Chionoecetes opilio Newfoundland National University of Ireland (NUI), Galway: ARAN ICES Journal of Marine Science 68 3 454 462 |
institution |
Open Polar |
collection |
National University of Ireland (NUI), Galway: ARAN |
op_collection_id |
ftnuigalway |
language |
unknown |
topic |
cancer pagurus diagnosis hematodinium sp histology pcr lobster nephrops-norvegicus crabs chionoecetes-opilio parasitic dinoflagellate hematodinium callinectes-sapidus blue-crab molecular-detection conception bay disease newfoundland prevalence |
spellingShingle |
cancer pagurus diagnosis hematodinium sp histology pcr lobster nephrops-norvegicus crabs chionoecetes-opilio parasitic dinoflagellate hematodinium callinectes-sapidus blue-crab molecular-detection conception bay disease newfoundland prevalence Ni Chualain, C. Robinson, M. Comparison of assessment methods used to diagnose hematodinium sp. infections in cancer pagurus |
topic_facet |
cancer pagurus diagnosis hematodinium sp histology pcr lobster nephrops-norvegicus crabs chionoecetes-opilio parasitic dinoflagellate hematodinium callinectes-sapidus blue-crab molecular-detection conception bay disease newfoundland prevalence |
description |
Endoparasitic dinoflagellates of the genus Hematodinium have recently gained attention as significant pathogens of the brown crab Cancer pagurus in Ireland. Patent infections, which are characterized by a hyperpigmented carapace and moribund condition, are limited to the discrete periods when macroscopic identification is possible. Three methods are assessed for diagnosing Hematodinium sp. infections in brown crab at the times when macroscopic identification is not always possible. Haemolymph smears, histological sections of gill, heart, midgut, hepatopancreas, muscle, and gonad, and a polymerase chain reaction (PCR) assay provided virtually equivalent accuracy in gauging infection prevalence, regardless of season. Sequences of PCR amplicons from the 18S ribosomal RNA gene confirmed the identity of the parasite as belonging to the genus Hematodinium. Infection intensity values (< 1-87%) obtained from haemolymph smears underscored infection levels within tissues, 90% of which contained advanced levels of infection. Alterations to tissues of infected crabs included haemocytopoenia, oedema, which caused dilation of the haemal sinuses resulting in pressure necrosis to the connective tissues around the oocytes, myocardial bundles, and hepatopancreatic tubules. The claw muscle of infected animals contained the fewest parasites. |
format |
Article in Journal/Newspaper |
author |
Ni Chualain, C. Robinson, M. |
author_facet |
Ni Chualain, C. Robinson, M. |
author_sort |
Ni Chualain, C. |
title |
Comparison of assessment methods used to diagnose hematodinium sp. infections in cancer pagurus |
title_short |
Comparison of assessment methods used to diagnose hematodinium sp. infections in cancer pagurus |
title_full |
Comparison of assessment methods used to diagnose hematodinium sp. infections in cancer pagurus |
title_fullStr |
Comparison of assessment methods used to diagnose hematodinium sp. infections in cancer pagurus |
title_full_unstemmed |
Comparison of assessment methods used to diagnose hematodinium sp. infections in cancer pagurus |
title_sort |
comparison of assessment methods used to diagnose hematodinium sp. infections in cancer pagurus |
publisher |
Oxford University Press (OUP) |
publishDate |
2011 |
url |
http://hdl.handle.net/10379/10783 https://doi.org/10.1093/icesjms/fsq197 |
genre |
Chionoecetes opilio Newfoundland |
genre_facet |
Chionoecetes opilio Newfoundland |
op_relation |
ICES Journal of Marine Science Ni Chualain, C. Robinson, M. (2011). Comparison of assessment methods used to diagnose hematodinium sp. infections in cancer pagurus. ICES Journal of Marine Science 68 (3), 454-462 1054-3139,1095-9289 http://hdl.handle.net/10379/10783 doi:10.1093/icesjms/fsq197 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ |
op_doi |
https://doi.org/10.1093/icesjms/fsq197 |
container_title |
ICES Journal of Marine Science |
container_volume |
68 |
container_issue |
3 |
container_start_page |
454 |
op_container_end_page |
462 |
_version_ |
1768385720890687488 |