Dietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (sparus aurata), but modulate the transcriptomic response to infection with enteromyxum leei
Background: Studies conducted with gilthead sea bream (Sparus aurata L.) have determined the maximum dietary replacement of fish meal and oil without compromising growth or product quality. The present study aimed to analyze the effect of the nutritional background on fish health and fish fed plant...
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Springer Nature
2012
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Online Access: | http://hdl.handle.net/10379/10651 https://doi.org/10.1186/1471-2164-13-470 |
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ftnuigalway:oai:aran.library.nuigalway.ie/:10379/10651 2023-06-11T04:10:22+02:00 Dietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (sparus aurata), but modulate the transcriptomic response to infection with enteromyxum leei Calduch-Giner, Josep A Sitjà-Bobadilla, Ariadna Davey, Grace C Cairns, Michael T Kaushik, Sadasivam Pérez-Sánchez, Jaume 2012-01-01 http://hdl.handle.net/10379/10651 https://doi.org/10.1186/1471-2164-13-470 unknown Springer Nature BMC Genomics Calduch-Giner, Josep A; Sitjà-Bobadilla, Ariadna; Davey, Grace C; Cairns, Michael T; Kaushik, Sadasivam; Pérez-Sánchez, Jaume (2012). Dietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (sparus aurata), but modulate the transcriptomic response to infection with enteromyxum leei. BMC Genomics 13 , 1471-2164 http://hdl.handle.net/10379/10651 doi:10.1186/1471-2164-13-470 Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ teleost parasite myxozoa intestine transcriptome nutrigenomics salmon salmo-salar trout oncorhynchus-mykiss orange-spotted grouper fatty-acid-composition immune relevant genes cyprinus-carpio l atlantic salmon rainbow-trout microarray technology fish-oil Article 2012 ftnuigalway https://doi.org/10.1186/1471-2164-13-470 2023-05-28T18:05:05Z Background: Studies conducted with gilthead sea bream (Sparus aurata L.) have determined the maximum dietary replacement of fish meal and oil without compromising growth or product quality. The present study aimed to analyze the effect of the nutritional background on fish health and fish fed plant protein-based diets with fish oil (FO diet) or a blend of vegetable oils (66VO diet) were exposed for 102 days to the intestinal myxosporean parasite Enteromyxum leei, and the intestine transcriptome was analyzed with a customized oligo-microarray of 7,500 annotated genes. Results: Infection prevalence was high and similar in the two diet groups, but the outcome of the disease was more pronounced in fish fed the 66VO diet. No differences were found in the transcriptome of both diet control groups, whereas the number of differentially expressed genes in infected groups was considerable. K-means clustering of these differentially expressed genes identified four expression patterns that reflected the progression of the disease with the magnitude of the fold-change being higher in infected 66VO fish. A positive correlation was found between the time of infection and the magnitude of the transcriptional change within the 66VO group, being higher in early infected animals. Within this diet group, a strong up-regulation of many components of the immune specific response was evidenced, whereas other genes related to complement response and xenobiotic metabolism were down-regulated. Conclusions: The high replacement of fish oil by vegetable oils in practical fish feeds did not modify the intestine transcriptome of gilthead sea bream, but important changes were apparent when fish were exposed to the myxosporean E. leei. The detected changes were mostly a consequence rather than a cause of the different disease progression in the two diet groups. Hence, the developed microarray constitutes an excellent diagnostic tool to address changes associated with the action of intestinal pathogens, but lacks a prognostic value to predict ... Article in Journal/Newspaper Atlantic salmon Salmo salar National University of Ireland (NUI), Galway: ARAN BMC Genomics 13 1 470 |
institution |
Open Polar |
collection |
National University of Ireland (NUI), Galway: ARAN |
op_collection_id |
ftnuigalway |
language |
unknown |
topic |
teleost parasite myxozoa intestine transcriptome nutrigenomics salmon salmo-salar trout oncorhynchus-mykiss orange-spotted grouper fatty-acid-composition immune relevant genes cyprinus-carpio l atlantic salmon rainbow-trout microarray technology fish-oil |
spellingShingle |
teleost parasite myxozoa intestine transcriptome nutrigenomics salmon salmo-salar trout oncorhynchus-mykiss orange-spotted grouper fatty-acid-composition immune relevant genes cyprinus-carpio l atlantic salmon rainbow-trout microarray technology fish-oil Calduch-Giner, Josep A Sitjà-Bobadilla, Ariadna Davey, Grace C Cairns, Michael T Kaushik, Sadasivam Pérez-Sánchez, Jaume Dietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (sparus aurata), but modulate the transcriptomic response to infection with enteromyxum leei |
topic_facet |
teleost parasite myxozoa intestine transcriptome nutrigenomics salmon salmo-salar trout oncorhynchus-mykiss orange-spotted grouper fatty-acid-composition immune relevant genes cyprinus-carpio l atlantic salmon rainbow-trout microarray technology fish-oil |
description |
Background: Studies conducted with gilthead sea bream (Sparus aurata L.) have determined the maximum dietary replacement of fish meal and oil without compromising growth or product quality. The present study aimed to analyze the effect of the nutritional background on fish health and fish fed plant protein-based diets with fish oil (FO diet) or a blend of vegetable oils (66VO diet) were exposed for 102 days to the intestinal myxosporean parasite Enteromyxum leei, and the intestine transcriptome was analyzed with a customized oligo-microarray of 7,500 annotated genes. Results: Infection prevalence was high and similar in the two diet groups, but the outcome of the disease was more pronounced in fish fed the 66VO diet. No differences were found in the transcriptome of both diet control groups, whereas the number of differentially expressed genes in infected groups was considerable. K-means clustering of these differentially expressed genes identified four expression patterns that reflected the progression of the disease with the magnitude of the fold-change being higher in infected 66VO fish. A positive correlation was found between the time of infection and the magnitude of the transcriptional change within the 66VO group, being higher in early infected animals. Within this diet group, a strong up-regulation of many components of the immune specific response was evidenced, whereas other genes related to complement response and xenobiotic metabolism were down-regulated. Conclusions: The high replacement of fish oil by vegetable oils in practical fish feeds did not modify the intestine transcriptome of gilthead sea bream, but important changes were apparent when fish were exposed to the myxosporean E. leei. The detected changes were mostly a consequence rather than a cause of the different disease progression in the two diet groups. Hence, the developed microarray constitutes an excellent diagnostic tool to address changes associated with the action of intestinal pathogens, but lacks a prognostic value to predict ... |
format |
Article in Journal/Newspaper |
author |
Calduch-Giner, Josep A Sitjà-Bobadilla, Ariadna Davey, Grace C Cairns, Michael T Kaushik, Sadasivam Pérez-Sánchez, Jaume |
author_facet |
Calduch-Giner, Josep A Sitjà-Bobadilla, Ariadna Davey, Grace C Cairns, Michael T Kaushik, Sadasivam Pérez-Sánchez, Jaume |
author_sort |
Calduch-Giner, Josep A |
title |
Dietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (sparus aurata), but modulate the transcriptomic response to infection with enteromyxum leei |
title_short |
Dietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (sparus aurata), but modulate the transcriptomic response to infection with enteromyxum leei |
title_full |
Dietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (sparus aurata), but modulate the transcriptomic response to infection with enteromyxum leei |
title_fullStr |
Dietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (sparus aurata), but modulate the transcriptomic response to infection with enteromyxum leei |
title_full_unstemmed |
Dietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (sparus aurata), but modulate the transcriptomic response to infection with enteromyxum leei |
title_sort |
dietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (sparus aurata), but modulate the transcriptomic response to infection with enteromyxum leei |
publisher |
Springer Nature |
publishDate |
2012 |
url |
http://hdl.handle.net/10379/10651 https://doi.org/10.1186/1471-2164-13-470 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
BMC Genomics Calduch-Giner, Josep A; Sitjà-Bobadilla, Ariadna; Davey, Grace C; Cairns, Michael T; Kaushik, Sadasivam; Pérez-Sánchez, Jaume (2012). Dietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (sparus aurata), but modulate the transcriptomic response to infection with enteromyxum leei. BMC Genomics 13 , 1471-2164 http://hdl.handle.net/10379/10651 doi:10.1186/1471-2164-13-470 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ |
op_doi |
https://doi.org/10.1186/1471-2164-13-470 |
container_title |
BMC Genomics |
container_volume |
13 |
container_issue |
1 |
container_start_page |
470 |
_version_ |
1768384721003216896 |