Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (sparus aurata l.)
Background: Selection programs for growth and stress traits in cultured fish are fundamental to the improvement of aquaculture production. The gilthead sea bream ( Sparus aurata) is the main aquacultured species in the Mediterranean area and there is considerable interest in the genetic improvement...
Published in: | BMC Genomics |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Springer Nature
2010
|
Subjects: | |
Online Access: | http://hdl.handle.net/10379/10650 https://doi.org/10.1186/1471-2164-11-193 |
id |
ftnuigalway:oai:aran.library.nuigalway.ie/:10379/10650 |
---|---|
record_format |
openpolar |
spelling |
ftnuigalway:oai:aran.library.nuigalway.ie/:10379/10650 2023-06-11T04:10:21+02:00 Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (sparus aurata l.) Calduch-Giner, Josep A Davey, Grace Saera-Vila, Alfonso Houeix, Benoit Talbot, Anita Prunet, Patrick Cairns, Michael T Perez-Sanchez, Jaume 2010-01-01 http://hdl.handle.net/10379/10650 https://doi.org/10.1186/1471-2164-11-193 unknown Springer Nature BMC Genomics Calduch-Giner, Josep A; Davey, Grace; Saera-Vila, Alfonso; Houeix, Benoit; Talbot, Anita; Prunet, Patrick; Cairns, Michael T; Perez-Sanchez, Jaume (2010). Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (sparus aurata l.). BMC Genomics 11 , 1471-2164 http://hdl.handle.net/10379/10650 doi:10.1186/1471-2164-11-193 Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ trout oncorhynchus-mykiss suppression subtractive hybridization unfolded protein response gene-expression profiles retinol-binding-protein rainbow-trout cortisol response oxidative stress atlantic salmon plasma-cortisol Article 2010 ftnuigalway https://doi.org/10.1186/1471-2164-11-193 2023-05-28T18:05:05Z Background: Selection programs for growth and stress traits in cultured fish are fundamental to the improvement of aquaculture production. The gilthead sea bream ( Sparus aurata) is the main aquacultured species in the Mediterranean area and there is considerable interest in the genetic improvement of this species. With the aim of increasing the genomic resources in gilthead sea bream and identifying genes and mechanisms underlying the physiology of the stress response, we developed a cDNA microarray for gilthead sea bream that is enriched by suppression substractive hybridization with stress and immunorelevant genes. This microarray is used to analyze the dynamics of gilthead sea bream liver expression profile after confinement exposure. Results: Groups of confined and control juvenile fish were sampled at 6, 24, 72 and 120 h post exposure. GeneSpring analyses identified 202 annotated genes that appeared differentially expressed at least at one sampling time (P < 0.05). Gene expression results were validated by quantitative PCR of 10 target genes, and K-means clustering of differently expressed genes identified four major temporal gene expression profiles. Set 1 encompassed a rapid metabolic readjustment with enhanced uptake and intracellular transport of fatty acids as metabolic fuels. Set 2 was associated with a wide variety of tissue repair and remodeling processes that were mostly mediated by the stress response of the endoplasmic reticulum ( ER). Sets 3 and 4 encompassed the re-establishment of cellular homeostasis with increased intracellular trafficking and scavenging of reactive oxygen species (ROS), accompanied by a bidirectional regulation of the immune system and a general decline of ROS production. Conclusions: Collectively, these findings show the complex nature of the adaptive stress response with a clear indication that the ER is an important control point for homeostatic adjustments. The study also identifies metabolic pathways which could be analyzed in greater detail to provide new insights ... Article in Journal/Newspaper Atlantic salmon National University of Ireland (NUI), Galway: ARAN BMC Genomics 11 1 193 |
institution |
Open Polar |
collection |
National University of Ireland (NUI), Galway: ARAN |
op_collection_id |
ftnuigalway |
language |
unknown |
topic |
trout oncorhynchus-mykiss suppression subtractive hybridization unfolded protein response gene-expression profiles retinol-binding-protein rainbow-trout cortisol response oxidative stress atlantic salmon plasma-cortisol |
spellingShingle |
trout oncorhynchus-mykiss suppression subtractive hybridization unfolded protein response gene-expression profiles retinol-binding-protein rainbow-trout cortisol response oxidative stress atlantic salmon plasma-cortisol Calduch-Giner, Josep A Davey, Grace Saera-Vila, Alfonso Houeix, Benoit Talbot, Anita Prunet, Patrick Cairns, Michael T Perez-Sanchez, Jaume Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (sparus aurata l.) |
topic_facet |
trout oncorhynchus-mykiss suppression subtractive hybridization unfolded protein response gene-expression profiles retinol-binding-protein rainbow-trout cortisol response oxidative stress atlantic salmon plasma-cortisol |
description |
Background: Selection programs for growth and stress traits in cultured fish are fundamental to the improvement of aquaculture production. The gilthead sea bream ( Sparus aurata) is the main aquacultured species in the Mediterranean area and there is considerable interest in the genetic improvement of this species. With the aim of increasing the genomic resources in gilthead sea bream and identifying genes and mechanisms underlying the physiology of the stress response, we developed a cDNA microarray for gilthead sea bream that is enriched by suppression substractive hybridization with stress and immunorelevant genes. This microarray is used to analyze the dynamics of gilthead sea bream liver expression profile after confinement exposure. Results: Groups of confined and control juvenile fish were sampled at 6, 24, 72 and 120 h post exposure. GeneSpring analyses identified 202 annotated genes that appeared differentially expressed at least at one sampling time (P < 0.05). Gene expression results were validated by quantitative PCR of 10 target genes, and K-means clustering of differently expressed genes identified four major temporal gene expression profiles. Set 1 encompassed a rapid metabolic readjustment with enhanced uptake and intracellular transport of fatty acids as metabolic fuels. Set 2 was associated with a wide variety of tissue repair and remodeling processes that were mostly mediated by the stress response of the endoplasmic reticulum ( ER). Sets 3 and 4 encompassed the re-establishment of cellular homeostasis with increased intracellular trafficking and scavenging of reactive oxygen species (ROS), accompanied by a bidirectional regulation of the immune system and a general decline of ROS production. Conclusions: Collectively, these findings show the complex nature of the adaptive stress response with a clear indication that the ER is an important control point for homeostatic adjustments. The study also identifies metabolic pathways which could be analyzed in greater detail to provide new insights ... |
format |
Article in Journal/Newspaper |
author |
Calduch-Giner, Josep A Davey, Grace Saera-Vila, Alfonso Houeix, Benoit Talbot, Anita Prunet, Patrick Cairns, Michael T Perez-Sanchez, Jaume |
author_facet |
Calduch-Giner, Josep A Davey, Grace Saera-Vila, Alfonso Houeix, Benoit Talbot, Anita Prunet, Patrick Cairns, Michael T Perez-Sanchez, Jaume |
author_sort |
Calduch-Giner, Josep A |
title |
Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (sparus aurata l.) |
title_short |
Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (sparus aurata l.) |
title_full |
Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (sparus aurata l.) |
title_fullStr |
Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (sparus aurata l.) |
title_full_unstemmed |
Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (sparus aurata l.) |
title_sort |
use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (sparus aurata l.) |
publisher |
Springer Nature |
publishDate |
2010 |
url |
http://hdl.handle.net/10379/10650 https://doi.org/10.1186/1471-2164-11-193 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
BMC Genomics Calduch-Giner, Josep A; Davey, Grace; Saera-Vila, Alfonso; Houeix, Benoit; Talbot, Anita; Prunet, Patrick; Cairns, Michael T; Perez-Sanchez, Jaume (2010). Use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (sparus aurata l.). BMC Genomics 11 , 1471-2164 http://hdl.handle.net/10379/10650 doi:10.1186/1471-2164-11-193 |
op_rights |
Attribution-NonCommercial-NoDerivs 3.0 Ireland https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ |
op_doi |
https://doi.org/10.1186/1471-2164-11-193 |
container_title |
BMC Genomics |
container_volume |
11 |
container_issue |
1 |
container_start_page |
193 |
_version_ |
1768384716637995008 |