A novel gnotobiotic experimental system for Atlantic salmon ( Salmo salar L.) reveals a microbial influence on mucosal barrier function and adipose tissue accumulation during the yolk sac stage
Gnotobiotic models have had a crucial role in studying the effect that commensal microbiota has on the health of their animal hosts. Despite their physiological and ecological diversity, teleost fishes are still underrepresented in gnotobiotic research. Moreover, a better understanding of host-micro...
Published in: | Frontiers in Cellular and Infection Microbiology |
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Online Access: | https://hdl.handle.net/11250/3101239 https://doi.org/10.3389/fcimb.2022.1068302 |
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ftntnutrondheimi:oai:ntnuopen.ntnu.no:11250/3101239 2023-12-10T09:46:52+01:00 A novel gnotobiotic experimental system for Atlantic salmon ( Salmo salar L.) reveals a microbial influence on mucosal barrier function and adipose tissue accumulation during the yolk sac stage Gomez de la Torre Canny, Sol Nordgård, Catherine Taylor Mathisen, Amalie johanne Horn Degré Lorentsen, Eirik Vadstein, Olav Bakke, Ingrid 2023 application/pdf https://hdl.handle.net/11250/3101239 https://doi.org/10.3389/fcimb.2022.1068302 eng eng Frontiers Media S. A. urn:issn:2235-2988 https://hdl.handle.net/11250/3101239 https://doi.org/10.3389/fcimb.2022.1068302 cristin:2155102 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no 12 Frontiers in Cellular and Infection Microbiology Peer reviewed Journal article 2023 ftntnutrondheimi https://doi.org/10.3389/fcimb.2022.1068302 2023-11-15T23:46:52Z Gnotobiotic models have had a crucial role in studying the effect that commensal microbiota has on the health of their animal hosts. Despite their physiological and ecological diversity, teleost fishes are still underrepresented in gnotobiotic research. Moreover, a better understanding of host-microbe interactions in farmed fish has the potential to contribute to sustainable global food supply. We have developed a novel gnotobiotic experimental system that includes the derivation of fertilized eggs of farmed and wild Atlantic salmon, and gnotobiotic husbandry of fry during the yolk sac stage. We used a microscopy-based approach to estimate the barrier function of the skin mucus layer and used this measurement to select the derivation procedure that minimized adverse effects on the skin mucosa. We also used this method to demonstrate that the mucus barrier was reduced in germ-free fry when compared to fry colonized with two different bacterial communities. This alteration in the mucus barrier was preceded by an increase in the number of cells containing neutral mucosubstances in the anterior segment of the body, but without changes in the number of cells containing acidic substances in any of the other segments studied along the body axis. In addition, we showed how the microbial status of the fry temporarily affected body size and the utilization of internal yolk stores during the yolk sac stage. Finally, we showed that the presence of bacterial communities associated with the fry, as well as their composition, affected the size of adipose tissue. Fry colonized with water from a lake had a larger visceral adipose tissue depot than both conventionally raised and germ-free fry. Together, our results show that this novel gnotobiotic experimental system is a useful tool for the study of host-microbe interactions in this species of aquacultural importance. publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar NTNU Open Archive (Norwegian University of Science and Technology) Frontiers in Cellular and Infection Microbiology 12 |
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Open Polar |
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NTNU Open Archive (Norwegian University of Science and Technology) |
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ftntnutrondheimi |
language |
English |
description |
Gnotobiotic models have had a crucial role in studying the effect that commensal microbiota has on the health of their animal hosts. Despite their physiological and ecological diversity, teleost fishes are still underrepresented in gnotobiotic research. Moreover, a better understanding of host-microbe interactions in farmed fish has the potential to contribute to sustainable global food supply. We have developed a novel gnotobiotic experimental system that includes the derivation of fertilized eggs of farmed and wild Atlantic salmon, and gnotobiotic husbandry of fry during the yolk sac stage. We used a microscopy-based approach to estimate the barrier function of the skin mucus layer and used this measurement to select the derivation procedure that minimized adverse effects on the skin mucosa. We also used this method to demonstrate that the mucus barrier was reduced in germ-free fry when compared to fry colonized with two different bacterial communities. This alteration in the mucus barrier was preceded by an increase in the number of cells containing neutral mucosubstances in the anterior segment of the body, but without changes in the number of cells containing acidic substances in any of the other segments studied along the body axis. In addition, we showed how the microbial status of the fry temporarily affected body size and the utilization of internal yolk stores during the yolk sac stage. Finally, we showed that the presence of bacterial communities associated with the fry, as well as their composition, affected the size of adipose tissue. Fry colonized with water from a lake had a larger visceral adipose tissue depot than both conventionally raised and germ-free fry. Together, our results show that this novel gnotobiotic experimental system is a useful tool for the study of host-microbe interactions in this species of aquacultural importance. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Gomez de la Torre Canny, Sol Nordgård, Catherine Taylor Mathisen, Amalie johanne Horn Degré Lorentsen, Eirik Vadstein, Olav Bakke, Ingrid |
spellingShingle |
Gomez de la Torre Canny, Sol Nordgård, Catherine Taylor Mathisen, Amalie johanne Horn Degré Lorentsen, Eirik Vadstein, Olav Bakke, Ingrid A novel gnotobiotic experimental system for Atlantic salmon ( Salmo salar L.) reveals a microbial influence on mucosal barrier function and adipose tissue accumulation during the yolk sac stage |
author_facet |
Gomez de la Torre Canny, Sol Nordgård, Catherine Taylor Mathisen, Amalie johanne Horn Degré Lorentsen, Eirik Vadstein, Olav Bakke, Ingrid |
author_sort |
Gomez de la Torre Canny, Sol |
title |
A novel gnotobiotic experimental system for Atlantic salmon ( Salmo salar L.) reveals a microbial influence on mucosal barrier function and adipose tissue accumulation during the yolk sac stage |
title_short |
A novel gnotobiotic experimental system for Atlantic salmon ( Salmo salar L.) reveals a microbial influence on mucosal barrier function and adipose tissue accumulation during the yolk sac stage |
title_full |
A novel gnotobiotic experimental system for Atlantic salmon ( Salmo salar L.) reveals a microbial influence on mucosal barrier function and adipose tissue accumulation during the yolk sac stage |
title_fullStr |
A novel gnotobiotic experimental system for Atlantic salmon ( Salmo salar L.) reveals a microbial influence on mucosal barrier function and adipose tissue accumulation during the yolk sac stage |
title_full_unstemmed |
A novel gnotobiotic experimental system for Atlantic salmon ( Salmo salar L.) reveals a microbial influence on mucosal barrier function and adipose tissue accumulation during the yolk sac stage |
title_sort |
novel gnotobiotic experimental system for atlantic salmon ( salmo salar l.) reveals a microbial influence on mucosal barrier function and adipose tissue accumulation during the yolk sac stage |
publisher |
Frontiers Media S. A. |
publishDate |
2023 |
url |
https://hdl.handle.net/11250/3101239 https://doi.org/10.3389/fcimb.2022.1068302 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
12 Frontiers in Cellular and Infection Microbiology |
op_relation |
urn:issn:2235-2988 https://hdl.handle.net/11250/3101239 https://doi.org/10.3389/fcimb.2022.1068302 cristin:2155102 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no |
op_doi |
https://doi.org/10.3389/fcimb.2022.1068302 |
container_title |
Frontiers in Cellular and Infection Microbiology |
container_volume |
12 |
_version_ |
1784890402452537344 |