Immersion Challenge of Atlantic Salmon (Salmo salar) yolk sac fry with Flavobacterium columnare
The microbiota is regarded as an essential part for most organisms’ development and health, especially through protection against pathogens, digestion and nutrient intake, and development of the immune system. However, the mechanics for such interaction are not fully understood yet. The ability of p...
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ftntnutrondheimi:oai:ntnuopen.ntnu.no:11250/3023774 2023-05-15T15:32:02+02:00 Immersion Challenge of Atlantic Salmon (Salmo salar) yolk sac fry with Flavobacterium columnare Vo, Toan. Bakke, Ingrid. Fiedler, Alexander. 2022 application/pdf https://hdl.handle.net/11250/3023774 eng eng NTNU no.ntnu:inspera:110629533:18762342 https://hdl.handle.net/11250/3023774 Master thesis 2022 ftntnutrondheimi 2022-10-05T22:41:52Z The microbiota is regarded as an essential part for most organisms’ development and health, especially through protection against pathogens, digestion and nutrient intake, and development of the immune system. However, the mechanics for such interaction are not fully understood yet. The ability of phages to infect and lyse certain target host bacteria has been used as a treatment against pathogens as well. However, there is only a limited number of studies on the protective effect of the microbiota against pathogens and phage therapy, and even less studies have been conducted in these fields in regard to fish such as Salmo salar. If the potential of phage therapy shows to be a promising tool against pathogens, it could be used as an alternative to antibiotics. In recent years, there has been an increasing focus on the emergence of antibiotics resistant bacteria, which pose a great threat in the medical and industrial fields of society. Gaining deeper understanding of microbiota host interactions and phage therapy could provide tools to further protecting animals, such as fish in aquaculture, from pathogens. To achieve this, a reproducible immersion challenge of Atlantic salmon yolk sac fry with a pathogen needed to be established. One of the goals of this project is to examine the pathogen Flavobacterium columnare strains UCD Fc7 and FCO-F2 as candidates for immersion challenge of the fry. Additionally, once an immersion challenge has been established, the potential protective effects of the microbiota and the viability of phage therapy was tested in immersion challenges. To examine the protective effects of the microbiota, germ-free fish and fish with a microbiota present were immersion challenged with F. columnare Fc7 and FCO-F2, additionally some fish were treated with FCL-2, a phage targeting F. columnare Fc7, and the mortality between groups were compared. It was shown that conventionalized fish were noticeably more resistant against the pathogen than germ-free fish, and that fry receiving phage treatment ... Master Thesis Atlantic salmon Salmo salar NTNU Open Archive (Norwegian University of Science and Technology) |
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NTNU Open Archive (Norwegian University of Science and Technology) |
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ftntnutrondheimi |
language |
English |
description |
The microbiota is regarded as an essential part for most organisms’ development and health, especially through protection against pathogens, digestion and nutrient intake, and development of the immune system. However, the mechanics for such interaction are not fully understood yet. The ability of phages to infect and lyse certain target host bacteria has been used as a treatment against pathogens as well. However, there is only a limited number of studies on the protective effect of the microbiota against pathogens and phage therapy, and even less studies have been conducted in these fields in regard to fish such as Salmo salar. If the potential of phage therapy shows to be a promising tool against pathogens, it could be used as an alternative to antibiotics. In recent years, there has been an increasing focus on the emergence of antibiotics resistant bacteria, which pose a great threat in the medical and industrial fields of society. Gaining deeper understanding of microbiota host interactions and phage therapy could provide tools to further protecting animals, such as fish in aquaculture, from pathogens. To achieve this, a reproducible immersion challenge of Atlantic salmon yolk sac fry with a pathogen needed to be established. One of the goals of this project is to examine the pathogen Flavobacterium columnare strains UCD Fc7 and FCO-F2 as candidates for immersion challenge of the fry. Additionally, once an immersion challenge has been established, the potential protective effects of the microbiota and the viability of phage therapy was tested in immersion challenges. To examine the protective effects of the microbiota, germ-free fish and fish with a microbiota present were immersion challenged with F. columnare Fc7 and FCO-F2, additionally some fish were treated with FCL-2, a phage targeting F. columnare Fc7, and the mortality between groups were compared. It was shown that conventionalized fish were noticeably more resistant against the pathogen than germ-free fish, and that fry receiving phage treatment ... |
author2 |
Bakke, Ingrid. Fiedler, Alexander. |
format |
Master Thesis |
author |
Vo, Toan. |
spellingShingle |
Vo, Toan. Immersion Challenge of Atlantic Salmon (Salmo salar) yolk sac fry with Flavobacterium columnare |
author_facet |
Vo, Toan. |
author_sort |
Vo, Toan. |
title |
Immersion Challenge of Atlantic Salmon (Salmo salar) yolk sac fry with Flavobacterium columnare |
title_short |
Immersion Challenge of Atlantic Salmon (Salmo salar) yolk sac fry with Flavobacterium columnare |
title_full |
Immersion Challenge of Atlantic Salmon (Salmo salar) yolk sac fry with Flavobacterium columnare |
title_fullStr |
Immersion Challenge of Atlantic Salmon (Salmo salar) yolk sac fry with Flavobacterium columnare |
title_full_unstemmed |
Immersion Challenge of Atlantic Salmon (Salmo salar) yolk sac fry with Flavobacterium columnare |
title_sort |
immersion challenge of atlantic salmon (salmo salar) yolk sac fry with flavobacterium columnare |
publisher |
NTNU |
publishDate |
2022 |
url |
https://hdl.handle.net/11250/3023774 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
no.ntnu:inspera:110629533:18762342 https://hdl.handle.net/11250/3023774 |
_version_ |
1766362540340674560 |