Plasmid-associated antimicrobial resistance and virulence genes in Escherichia coli in a high arctic reindeer subspecies
Objectives In extreme environments, such as the Arctic region, the anthropogenic influence is low and the presence of antimicrobial-resistant bacteria is unexpected. In this study, we screened wild reindeer (Rangifer tarandus platyrhynchus) from the Svalbard High Arctic Archipelago for antimicrobial...
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ftntnutrondheimi:oai:ntnuopen.ntnu.no:11250/2829859 2023-05-15T14:28:54+02:00 Plasmid-associated antimicrobial resistance and virulence genes in Escherichia coli in a high arctic reindeer subspecies Sunde, Marianne Ramstad, Silje N. Rudi, Knut Porcellato, Davide Ravi, Anuradha Ludvigsen, Jane das Neves, Carlos Gonçalo Tryland, Morten Ropstad, Erik Slettemeås, Jannice S. Telke, Amar A. 2021 application/pdf https://hdl.handle.net/11250/2829859 https://doi.org/10.1016/j.jgar.2021.06.003 eng eng Elsevier Journal of Global Antimicrobial Resistance. 2021, 26 317-322. urn:issn:2213-7165 https://hdl.handle.net/11250/2829859 https://doi.org/10.1016/j.jgar.2021.06.003 cristin:1947806 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no CC-BY 317-322 26 Journal of Global Antimicrobial Resistance Peer reviewed Journal article 2021 ftntnutrondheimi https://doi.org/10.1016/j.jgar.2021.06.003 2021-11-17T23:36:04Z Objectives In extreme environments, such as the Arctic region, the anthropogenic influence is low and the presence of antimicrobial-resistant bacteria is unexpected. In this study, we screened wild reindeer (Rangifer tarandus platyrhynchus) from the Svalbard High Arctic Archipelago for antimicrobial-resistant Escherichia coli and performed in-depth strain characterisation. Methods Using selective culturing of faecal samples from 55 animals, resistant E. coli were isolated and subjected to minimum inhibitory concentration (MIC) determination, conjugation experiments and whole-genome sequencing. Results Twelve animals carried antimicrobial-resistant E. coli. Genomic analysis showed IncF plasmids as vectors both for resistance and virulence genes in most strains. Plasmid-associated genes encoding resistance to ampicillin, sulfonamides, streptomycin and trimethoprim were found in addition to virulence genes typical for colicin V (ColV)-producing plasmids. Comparison with previously reported IncF ColV plasmids from human and animal hosts showed high genetic similarity. The plasmids were detected in E. coli sequence types (STs) previously described as hosts for such plasmids, such as ST58, ST88 and ST131. Conclusion Antimicrobial-resistant E. coli were detected from Svalbard reindeer. Our findings show that successful hybrid antimicrobial resistance–ColV plasmids and their host strains are widely distributed also occurring in extreme environmental niches such as arctic ecosystems. Possible introduction routes of resistant bacterial strains and plasmids into Svalbard ecosystems may be through migrating birds, marine fish or mammals, arctic fox (Vulpes lagopus) or via human anthropogenic activities such as tourism. publishedVersion Article in Journal/Newspaper Arctic Archipelago Arctic Fox Arctic Rangifer tarandus Rangifer tarandus platyrhynchus Svalbard svalbard reindeer Vulpes lagopus NTNU Open Archive (Norwegian University of Science and Technology) Arctic Svalbard Journal of Global Antimicrobial Resistance 26 317 322 |
institution |
Open Polar |
collection |
NTNU Open Archive (Norwegian University of Science and Technology) |
op_collection_id |
ftntnutrondheimi |
language |
English |
description |
Objectives In extreme environments, such as the Arctic region, the anthropogenic influence is low and the presence of antimicrobial-resistant bacteria is unexpected. In this study, we screened wild reindeer (Rangifer tarandus platyrhynchus) from the Svalbard High Arctic Archipelago for antimicrobial-resistant Escherichia coli and performed in-depth strain characterisation. Methods Using selective culturing of faecal samples from 55 animals, resistant E. coli were isolated and subjected to minimum inhibitory concentration (MIC) determination, conjugation experiments and whole-genome sequencing. Results Twelve animals carried antimicrobial-resistant E. coli. Genomic analysis showed IncF plasmids as vectors both for resistance and virulence genes in most strains. Plasmid-associated genes encoding resistance to ampicillin, sulfonamides, streptomycin and trimethoprim were found in addition to virulence genes typical for colicin V (ColV)-producing plasmids. Comparison with previously reported IncF ColV plasmids from human and animal hosts showed high genetic similarity. The plasmids were detected in E. coli sequence types (STs) previously described as hosts for such plasmids, such as ST58, ST88 and ST131. Conclusion Antimicrobial-resistant E. coli were detected from Svalbard reindeer. Our findings show that successful hybrid antimicrobial resistance–ColV plasmids and their host strains are widely distributed also occurring in extreme environmental niches such as arctic ecosystems. Possible introduction routes of resistant bacterial strains and plasmids into Svalbard ecosystems may be through migrating birds, marine fish or mammals, arctic fox (Vulpes lagopus) or via human anthropogenic activities such as tourism. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Sunde, Marianne Ramstad, Silje N. Rudi, Knut Porcellato, Davide Ravi, Anuradha Ludvigsen, Jane das Neves, Carlos Gonçalo Tryland, Morten Ropstad, Erik Slettemeås, Jannice S. Telke, Amar A. |
spellingShingle |
Sunde, Marianne Ramstad, Silje N. Rudi, Knut Porcellato, Davide Ravi, Anuradha Ludvigsen, Jane das Neves, Carlos Gonçalo Tryland, Morten Ropstad, Erik Slettemeås, Jannice S. Telke, Amar A. Plasmid-associated antimicrobial resistance and virulence genes in Escherichia coli in a high arctic reindeer subspecies |
author_facet |
Sunde, Marianne Ramstad, Silje N. Rudi, Knut Porcellato, Davide Ravi, Anuradha Ludvigsen, Jane das Neves, Carlos Gonçalo Tryland, Morten Ropstad, Erik Slettemeås, Jannice S. Telke, Amar A. |
author_sort |
Sunde, Marianne |
title |
Plasmid-associated antimicrobial resistance and virulence genes in Escherichia coli in a high arctic reindeer subspecies |
title_short |
Plasmid-associated antimicrobial resistance and virulence genes in Escherichia coli in a high arctic reindeer subspecies |
title_full |
Plasmid-associated antimicrobial resistance and virulence genes in Escherichia coli in a high arctic reindeer subspecies |
title_fullStr |
Plasmid-associated antimicrobial resistance and virulence genes in Escherichia coli in a high arctic reindeer subspecies |
title_full_unstemmed |
Plasmid-associated antimicrobial resistance and virulence genes in Escherichia coli in a high arctic reindeer subspecies |
title_sort |
plasmid-associated antimicrobial resistance and virulence genes in escherichia coli in a high arctic reindeer subspecies |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://hdl.handle.net/11250/2829859 https://doi.org/10.1016/j.jgar.2021.06.003 |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic Archipelago Arctic Fox Arctic Rangifer tarandus Rangifer tarandus platyrhynchus Svalbard svalbard reindeer Vulpes lagopus |
genre_facet |
Arctic Archipelago Arctic Fox Arctic Rangifer tarandus Rangifer tarandus platyrhynchus Svalbard svalbard reindeer Vulpes lagopus |
op_source |
317-322 26 Journal of Global Antimicrobial Resistance |
op_relation |
Journal of Global Antimicrobial Resistance. 2021, 26 317-322. urn:issn:2213-7165 https://hdl.handle.net/11250/2829859 https://doi.org/10.1016/j.jgar.2021.06.003 cristin:1947806 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1016/j.jgar.2021.06.003 |
container_title |
Journal of Global Antimicrobial Resistance |
container_volume |
26 |
container_start_page |
317 |
op_container_end_page |
322 |
_version_ |
1766303030850879488 |