Rapid Determination of Per- and Polyfluoroalkyl Substances (PFAS) in Harbour Porpoise Liver Tissue by HybridSPE®–UPLC®–MS/MS
A rapid hybrid solid phase extraction (HybridSPE®) protocol tailored to ultra-performance liquid chromatography–electrospray ionization tandem mass spectrometry (UPLC®–ESI–MS/MS) analysis was developed for the determination of 15 per- and polyfluoroalkyl substances (PFAS) in liver tissue from harbou...
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Online Access: | https://hdl.handle.net/11250/2789365 https://doi.org/10.3390/toxics9080183 |
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ftntnutrondheimi:oai:ntnuopen.ntnu.no:11250/2789365 2023-05-15T16:33:24+02:00 Rapid Determination of Per- and Polyfluoroalkyl Substances (PFAS) in Harbour Porpoise Liver Tissue by HybridSPE®–UPLC®–MS/MS Trimmel, Simone Vike-Jonas, Kristine Gonzalez, Susana Villa Ciesielski, Tomasz Maciej Lindstrøm, Ulf Jenssen, Bjørn Munro Asimakopoulos, Alexandros 2021 application/pdf https://hdl.handle.net/11250/2789365 https://doi.org/10.3390/toxics9080183 eng eng MDPI Toxics. 2021, 9 (8), . urn:issn:2305-6304 https://hdl.handle.net/11250/2789365 https://doi.org/10.3390/toxics9080183 cristin:1926662 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no CC-BY 11 9 Toxics 8 Journal article Peer reviewed 2021 ftntnutrondheimi https://doi.org/10.3390/toxics9080183 2021-10-13T22:35:30Z A rapid hybrid solid phase extraction (HybridSPE®) protocol tailored to ultra-performance liquid chromatography–electrospray ionization tandem mass spectrometry (UPLC®–ESI–MS/MS) analysis was developed for the determination of 15 per- and polyfluoroalkyl substances (PFAS) in liver tissue from harbour porpoises (Phocoena phocoena). The HybridSPE® technique has been applied in trace concentration bioanalysis, but it was mainly used for liquid biological media until now. In this study, the protocol was applied on tissue matrix, and it demonstrated acceptable absolute recoveries (%) ranging from 44.4 to 89.4%. The chromatographic separation was carried out using a gradient elution program with a total run time of 4 min. The inter-day method precision ranged from 2.15 to 15.4%, and the method limits of detection (LODs) ranged from 0.003 to 0.30 ng/g wet weight (w.w.). A total of 20 liver samples were analyzed to demonstrate the applicability of the developed method in liver tissue from a wildlife species. publishedVersion Article in Journal/Newspaper Harbour porpoise Phocoena phocoena NTNU Open Archive (Norwegian University of Science and Technology) Toxics 9 8 183 |
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Open Polar |
collection |
NTNU Open Archive (Norwegian University of Science and Technology) |
op_collection_id |
ftntnutrondheimi |
language |
English |
description |
A rapid hybrid solid phase extraction (HybridSPE®) protocol tailored to ultra-performance liquid chromatography–electrospray ionization tandem mass spectrometry (UPLC®–ESI–MS/MS) analysis was developed for the determination of 15 per- and polyfluoroalkyl substances (PFAS) in liver tissue from harbour porpoises (Phocoena phocoena). The HybridSPE® technique has been applied in trace concentration bioanalysis, but it was mainly used for liquid biological media until now. In this study, the protocol was applied on tissue matrix, and it demonstrated acceptable absolute recoveries (%) ranging from 44.4 to 89.4%. The chromatographic separation was carried out using a gradient elution program with a total run time of 4 min. The inter-day method precision ranged from 2.15 to 15.4%, and the method limits of detection (LODs) ranged from 0.003 to 0.30 ng/g wet weight (w.w.). A total of 20 liver samples were analyzed to demonstrate the applicability of the developed method in liver tissue from a wildlife species. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Trimmel, Simone Vike-Jonas, Kristine Gonzalez, Susana Villa Ciesielski, Tomasz Maciej Lindstrøm, Ulf Jenssen, Bjørn Munro Asimakopoulos, Alexandros |
spellingShingle |
Trimmel, Simone Vike-Jonas, Kristine Gonzalez, Susana Villa Ciesielski, Tomasz Maciej Lindstrøm, Ulf Jenssen, Bjørn Munro Asimakopoulos, Alexandros Rapid Determination of Per- and Polyfluoroalkyl Substances (PFAS) in Harbour Porpoise Liver Tissue by HybridSPE®–UPLC®–MS/MS |
author_facet |
Trimmel, Simone Vike-Jonas, Kristine Gonzalez, Susana Villa Ciesielski, Tomasz Maciej Lindstrøm, Ulf Jenssen, Bjørn Munro Asimakopoulos, Alexandros |
author_sort |
Trimmel, Simone |
title |
Rapid Determination of Per- and Polyfluoroalkyl Substances (PFAS) in Harbour Porpoise Liver Tissue by HybridSPE®–UPLC®–MS/MS |
title_short |
Rapid Determination of Per- and Polyfluoroalkyl Substances (PFAS) in Harbour Porpoise Liver Tissue by HybridSPE®–UPLC®–MS/MS |
title_full |
Rapid Determination of Per- and Polyfluoroalkyl Substances (PFAS) in Harbour Porpoise Liver Tissue by HybridSPE®–UPLC®–MS/MS |
title_fullStr |
Rapid Determination of Per- and Polyfluoroalkyl Substances (PFAS) in Harbour Porpoise Liver Tissue by HybridSPE®–UPLC®–MS/MS |
title_full_unstemmed |
Rapid Determination of Per- and Polyfluoroalkyl Substances (PFAS) in Harbour Porpoise Liver Tissue by HybridSPE®–UPLC®–MS/MS |
title_sort |
rapid determination of per- and polyfluoroalkyl substances (pfas) in harbour porpoise liver tissue by hybridspe®–uplc®–ms/ms |
publisher |
MDPI |
publishDate |
2021 |
url |
https://hdl.handle.net/11250/2789365 https://doi.org/10.3390/toxics9080183 |
genre |
Harbour porpoise Phocoena phocoena |
genre_facet |
Harbour porpoise Phocoena phocoena |
op_source |
11 9 Toxics 8 |
op_relation |
Toxics. 2021, 9 (8), . urn:issn:2305-6304 https://hdl.handle.net/11250/2789365 https://doi.org/10.3390/toxics9080183 cristin:1926662 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/toxics9080183 |
container_title |
Toxics |
container_volume |
9 |
container_issue |
8 |
container_start_page |
183 |
_version_ |
1766023096432590848 |