Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic
High levels of organochlorines (OCs) have been measured in arctic char (Salvelinus alpinus) from Lake Ellasjøen on Bjørnøya, Norway (74.30°N, 19.0°E). In a nearby lake, Laksvatn, the OC-levels in arctic char were low. A previous study has shown that char from Ellasjøen had significantly higher level...
Published in: | Ecotoxicology and Environmental Safety |
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Online Access: | https://hdl.handle.net/11250/2726780 https://doi.org/10.1016/j.ecoenv.2020.111846 |
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ftntnutrondheimi:oai:ntnuopen.ntnu.no:11250/2726780 2023-05-15T14:54:25+02:00 Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic Inderberg, Helene Neerland, Eirik D. Mcpartland, Molly Sparstad, Torfinn Bytingsvik, Jenny Nikiforov, Vladimir A Evenset, Anita Krøkje, Åse 2021 application/pdf https://hdl.handle.net/11250/2726780 https://doi.org/10.1016/j.ecoenv.2020.111846 eng eng Elsevier urn:issn:0147-6513 https://hdl.handle.net/11250/2726780 https://doi.org/10.1016/j.ecoenv.2020.111846 cristin:1870950 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no CC-BY-NC-ND 210 Ecotoxicology and Environmental Safety Peer reviewed Journal article 2021 ftntnutrondheimi https://doi.org/10.1016/j.ecoenv.2020.111846 2021-02-10T23:35:03Z High levels of organochlorines (OCs) have been measured in arctic char (Salvelinus alpinus) from Lake Ellasjøen on Bjørnøya, Norway (74.30°N, 19.0°E). In a nearby lake, Laksvatn, the OC-levels in arctic char were low. A previous study has shown that char from Ellasjøen had significantly higher levels of DNA double strand breaks (DSBs) than char from Lake Laksvatn. Even though there is increasing evidence of the genotoxic effects of OCs, little is known about the effects of OCs on the DNA repair system. The aim of the present study was to determine if the two main DNA DSB repair mechanisms, homologous recombination (HR) and non-homologous end-joining (NHEJ), are affected by the higher OC and DSB level in char from Ellasjøen. This was analysed by comparing the transcript level of 11 genes involved in DNA DSB repair in char liver samples from Ellasjøen (n = 9) with char from Laksvatn (n = 12). Six of the investigated genes were significantly upregulated in char from Ellasjøen. As the expression of DNA DSB repair genes was increased in the contaminant-exposed char, it is likely that the DNA DSB repair capacity is induced in these individuals. This induction was positively correlated with the DNA DSB and negatively correlated with one or several OCs for four of these genes. However, the strongest predictor variable for DNA repair genes was habitat, indicating genetic differences in repair capacity between populations. As char from Ellasjøen still had significantly higher levels of DSBs compared to char from Laksvatn, it is possible that chronic exposure to OCs and continued production of DSB has caused selective pressure within the population for fixation of adaptive alleles. It is also possible that DSB production was exceeding the repair capacity given the prevailing conditions, or that the OC or DSB level was above the threshold value of inhibition of the DNA repair system resulting in the rate of DNA damage exceeding the rate of repair. publishedVersion © 2020 Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Article in Journal/Newspaper Arctic Bjørnøya Bjørnøya Salvelinus alpinus NTNU Open Archive (Norwegian University of Science and Technology) Arctic Bjørnøya ENVELOPE(-67.250,-67.250,-68.151,-68.151) Ellasjøen ENVELOPE(19.031,19.031,74.388,74.388) Laksvatn ENVELOPE(19.360,19.360,69.361,69.361) Norway Ecotoxicology and Environmental Safety 210 111846 |
institution |
Open Polar |
collection |
NTNU Open Archive (Norwegian University of Science and Technology) |
op_collection_id |
ftntnutrondheimi |
language |
English |
description |
High levels of organochlorines (OCs) have been measured in arctic char (Salvelinus alpinus) from Lake Ellasjøen on Bjørnøya, Norway (74.30°N, 19.0°E). In a nearby lake, Laksvatn, the OC-levels in arctic char were low. A previous study has shown that char from Ellasjøen had significantly higher levels of DNA double strand breaks (DSBs) than char from Lake Laksvatn. Even though there is increasing evidence of the genotoxic effects of OCs, little is known about the effects of OCs on the DNA repair system. The aim of the present study was to determine if the two main DNA DSB repair mechanisms, homologous recombination (HR) and non-homologous end-joining (NHEJ), are affected by the higher OC and DSB level in char from Ellasjøen. This was analysed by comparing the transcript level of 11 genes involved in DNA DSB repair in char liver samples from Ellasjøen (n = 9) with char from Laksvatn (n = 12). Six of the investigated genes were significantly upregulated in char from Ellasjøen. As the expression of DNA DSB repair genes was increased in the contaminant-exposed char, it is likely that the DNA DSB repair capacity is induced in these individuals. This induction was positively correlated with the DNA DSB and negatively correlated with one or several OCs for four of these genes. However, the strongest predictor variable for DNA repair genes was habitat, indicating genetic differences in repair capacity between populations. As char from Ellasjøen still had significantly higher levels of DSBs compared to char from Laksvatn, it is possible that chronic exposure to OCs and continued production of DSB has caused selective pressure within the population for fixation of adaptive alleles. It is also possible that DSB production was exceeding the repair capacity given the prevailing conditions, or that the OC or DSB level was above the threshold value of inhibition of the DNA repair system resulting in the rate of DNA damage exceeding the rate of repair. publishedVersion © 2020 Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
format |
Article in Journal/Newspaper |
author |
Inderberg, Helene Neerland, Eirik D. Mcpartland, Molly Sparstad, Torfinn Bytingsvik, Jenny Nikiforov, Vladimir A Evenset, Anita Krøkje, Åse |
spellingShingle |
Inderberg, Helene Neerland, Eirik D. Mcpartland, Molly Sparstad, Torfinn Bytingsvik, Jenny Nikiforov, Vladimir A Evenset, Anita Krøkje, Åse Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
author_facet |
Inderberg, Helene Neerland, Eirik D. Mcpartland, Molly Sparstad, Torfinn Bytingsvik, Jenny Nikiforov, Vladimir A Evenset, Anita Krøkje, Åse |
author_sort |
Inderberg, Helene |
title |
Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_short |
Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_full |
Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_fullStr |
Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_full_unstemmed |
Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_sort |
expression of dna repair genes in arctic char (salvelinus alpinus) from bjørnøya in the norwegian arctic |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://hdl.handle.net/11250/2726780 https://doi.org/10.1016/j.ecoenv.2020.111846 |
long_lat |
ENVELOPE(-67.250,-67.250,-68.151,-68.151) ENVELOPE(19.031,19.031,74.388,74.388) ENVELOPE(19.360,19.360,69.361,69.361) |
geographic |
Arctic Bjørnøya Ellasjøen Laksvatn Norway |
geographic_facet |
Arctic Bjørnøya Ellasjøen Laksvatn Norway |
genre |
Arctic Bjørnøya Bjørnøya Salvelinus alpinus |
genre_facet |
Arctic Bjørnøya Bjørnøya Salvelinus alpinus |
op_source |
210 Ecotoxicology and Environmental Safety |
op_relation |
urn:issn:0147-6513 https://hdl.handle.net/11250/2726780 https://doi.org/10.1016/j.ecoenv.2020.111846 cristin:1870950 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.ecoenv.2020.111846 |
container_title |
Ecotoxicology and Environmental Safety |
container_volume |
210 |
container_start_page |
111846 |
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1766326154421075968 |