Production, characterization, and application of an alginate lyase, AMOR_PL7A, from hot vents in the Arctic mid-ocean ridge
Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latis...
Published in: | Journal of Agricultural and Food Chemistry |
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Online Access: | https://hdl.handle.net/11250/2651994 https://doi.org/10.1021/acs.jafc.8b07190 |
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ftntnutrondheimi:oai:ntnuopen.ntnu.no:11250/2651994 2023-05-15T15:06:15+02:00 Production, characterization, and application of an alginate lyase, AMOR_PL7A, from hot vents in the Arctic mid-ocean ridge Vuoristo, Kiira Fredriksen, Lasse Oftebro, Maren Arntzen, Magnus Øverlie Aarstad, Olav Andreas Stokke, Runar Steen, Ida Helene Hansen, Line Degn Schüller, Reidar Barfod Aachmann, Finn Lillelund Horn, Svein Jarle Eijsink, Vincent 2019 application/pdf https://hdl.handle.net/11250/2651994 https://doi.org/10.1021/acs.jafc.8b07190 eng eng American Chemical Society Norges forskningsråd: 239003 Norges forskningsråd: 270038 Norges forskningsråd: 237841 Norges forskningsråd: 226247 Norges forskningsråd: 229003 Journal of Agricultural and Food Chemistry. 2019, 67 (10), 2936-2945. urn:issn:0021-8561 https://hdl.handle.net/11250/2651994 https://doi.org/10.1021/acs.jafc.8b07190 cristin:1704834 2936-2945 67 Journal of Agricultural and Food Chemistry 10 Peer reviewed Journal article 2019 ftntnutrondheimi https://doi.org/10.1021/acs.jafc.8b07190 2020-04-29T22:32:43Z Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR_PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and kcat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s–1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR_PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR_PL7A may be useful in development of efficient protocols for enzymatic seaweed processing. acceptedVersion This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Journal of Agricultural and Food Chemistry], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.jafc.8b07190 Article in Journal/Newspaper Arctic NTNU Open Archive (Norwegian University of Science and Technology) Arctic Journal of Agricultural and Food Chemistry 67 10 2936 2945 |
institution |
Open Polar |
collection |
NTNU Open Archive (Norwegian University of Science and Technology) |
op_collection_id |
ftntnutrondheimi |
language |
English |
description |
Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR_PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and kcat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s–1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR_PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR_PL7A may be useful in development of efficient protocols for enzymatic seaweed processing. acceptedVersion This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Journal of Agricultural and Food Chemistry], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.jafc.8b07190 |
format |
Article in Journal/Newspaper |
author |
Vuoristo, Kiira Fredriksen, Lasse Oftebro, Maren Arntzen, Magnus Øverlie Aarstad, Olav Andreas Stokke, Runar Steen, Ida Helene Hansen, Line Degn Schüller, Reidar Barfod Aachmann, Finn Lillelund Horn, Svein Jarle Eijsink, Vincent |
spellingShingle |
Vuoristo, Kiira Fredriksen, Lasse Oftebro, Maren Arntzen, Magnus Øverlie Aarstad, Olav Andreas Stokke, Runar Steen, Ida Helene Hansen, Line Degn Schüller, Reidar Barfod Aachmann, Finn Lillelund Horn, Svein Jarle Eijsink, Vincent Production, characterization, and application of an alginate lyase, AMOR_PL7A, from hot vents in the Arctic mid-ocean ridge |
author_facet |
Vuoristo, Kiira Fredriksen, Lasse Oftebro, Maren Arntzen, Magnus Øverlie Aarstad, Olav Andreas Stokke, Runar Steen, Ida Helene Hansen, Line Degn Schüller, Reidar Barfod Aachmann, Finn Lillelund Horn, Svein Jarle Eijsink, Vincent |
author_sort |
Vuoristo, Kiira |
title |
Production, characterization, and application of an alginate lyase, AMOR_PL7A, from hot vents in the Arctic mid-ocean ridge |
title_short |
Production, characterization, and application of an alginate lyase, AMOR_PL7A, from hot vents in the Arctic mid-ocean ridge |
title_full |
Production, characterization, and application of an alginate lyase, AMOR_PL7A, from hot vents in the Arctic mid-ocean ridge |
title_fullStr |
Production, characterization, and application of an alginate lyase, AMOR_PL7A, from hot vents in the Arctic mid-ocean ridge |
title_full_unstemmed |
Production, characterization, and application of an alginate lyase, AMOR_PL7A, from hot vents in the Arctic mid-ocean ridge |
title_sort |
production, characterization, and application of an alginate lyase, amor_pl7a, from hot vents in the arctic mid-ocean ridge |
publisher |
American Chemical Society |
publishDate |
2019 |
url |
https://hdl.handle.net/11250/2651994 https://doi.org/10.1021/acs.jafc.8b07190 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
2936-2945 67 Journal of Agricultural and Food Chemistry 10 |
op_relation |
Norges forskningsråd: 239003 Norges forskningsråd: 270038 Norges forskningsråd: 237841 Norges forskningsråd: 226247 Norges forskningsråd: 229003 Journal of Agricultural and Food Chemistry. 2019, 67 (10), 2936-2945. urn:issn:0021-8561 https://hdl.handle.net/11250/2651994 https://doi.org/10.1021/acs.jafc.8b07190 cristin:1704834 |
op_doi |
https://doi.org/10.1021/acs.jafc.8b07190 |
container_title |
Journal of Agricultural and Food Chemistry |
container_volume |
67 |
container_issue |
10 |
container_start_page |
2936 |
op_container_end_page |
2945 |
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1766337896461107200 |