DNA double‐strand breaks in Arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic
High levels of organochlorine contaminants (OCs) have been found in arctic char (Salvelinus alpinus) from Lake Ellasjøen, Bjørnøya (Norwegian Arctic). The aim of the present study was to investigate the potential genotoxic effect of environmental organochlorine contaminant exposure in arctic char fr...
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Online Access: | http://hdl.handle.net/11250/2637780 https://doi.org/10.1002/etc.4546 |
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ftntnutrondheimi:oai:ntnuopen.ntnu.no:11250/2637780 2023-05-15T14:33:14+02:00 DNA double‐strand breaks in Arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic Neerland, Eirik D. Bytingsvik, Jenny Nikiforov, Vladimir Evenset, Anita Krøkje, Åse 2019 http://hdl.handle.net/11250/2637780 https://doi.org/10.1002/etc.4546 eng eng Wiley Norges forskningsråd: 221371 Framsenteret: Flagship "Hazardous substances" Environmental Toxicology and Chemistry. 2019, 38 (11), 2405-2413. urn:issn:0730-7268 http://hdl.handle.net/11250/2637780 https://doi.org/10.1002/etc.4546 cristin:1735983 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no CC-BY 2405-2413 38 Environmental Toxicology and Chemistry 11 Journal article Peer reviewed 2019 ftntnutrondheimi https://doi.org/10.1002/etc.4546 2020-01-29T23:32:25Z High levels of organochlorine contaminants (OCs) have been found in arctic char (Salvelinus alpinus) from Lake Ellasjøen, Bjørnøya (Norwegian Arctic). The aim of the present study was to investigate the potential genotoxic effect of environmental organochlorine contaminant exposure in arctic char from Ellasjøen compared with arctic char from the low‐contaminated Lake Laksvatn nearby. Blood was analyzed using agarose gel electrophoresis and image data analysis to quantify the fraction of total DNA that migrated into the gel (DNA‐FTM) as a relative measure of DNA double‐strand breaks (DSBs). Analysis by GC‐MS of muscle samples showed an average 43 times higher concentration of ΣOCs in arctic char from Ellasjøen (n = 18) compared with Laksvatn char (n = 21). Char from Lake Ellasjøen had a much higher frequency of DSBs, as measured by DNA‐FTM, than char from Lake Laksvatn. Principal component analysis and multiple linear regressions show that there was a significant positive relationship between DSBs and levels of organochlorine contaminants in the char. In addition, DSBs were less frequent in reproductively mature char than in immature char. The results suggest that organochlorine contaminants are genotoxic to arctic char. DNA double‐strand breaks in Arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic publishedVersion © 2019 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Article in Journal/Newspaper Arctic Bjørnøya Bjørnøya Salvelinus alpinus NTNU Open Archive (Norwegian University of Science and Technology) Arctic Bjørnøya ENVELOPE(-67.250,-67.250,-68.151,-68.151) Ellasjøen ENVELOPE(19.031,19.031,74.388,74.388) Laksvatn ENVELOPE(19.360,19.360,69.361,69.361) Environmental Toxicology and Chemistry 38 11 2405 2413 |
institution |
Open Polar |
collection |
NTNU Open Archive (Norwegian University of Science and Technology) |
op_collection_id |
ftntnutrondheimi |
language |
English |
description |
High levels of organochlorine contaminants (OCs) have been found in arctic char (Salvelinus alpinus) from Lake Ellasjøen, Bjørnøya (Norwegian Arctic). The aim of the present study was to investigate the potential genotoxic effect of environmental organochlorine contaminant exposure in arctic char from Ellasjøen compared with arctic char from the low‐contaminated Lake Laksvatn nearby. Blood was analyzed using agarose gel electrophoresis and image data analysis to quantify the fraction of total DNA that migrated into the gel (DNA‐FTM) as a relative measure of DNA double‐strand breaks (DSBs). Analysis by GC‐MS of muscle samples showed an average 43 times higher concentration of ΣOCs in arctic char from Ellasjøen (n = 18) compared with Laksvatn char (n = 21). Char from Lake Ellasjøen had a much higher frequency of DSBs, as measured by DNA‐FTM, than char from Lake Laksvatn. Principal component analysis and multiple linear regressions show that there was a significant positive relationship between DSBs and levels of organochlorine contaminants in the char. In addition, DSBs were less frequent in reproductively mature char than in immature char. The results suggest that organochlorine contaminants are genotoxic to arctic char. DNA double‐strand breaks in Arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic publishedVersion © 2019 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
format |
Article in Journal/Newspaper |
author |
Neerland, Eirik D. Bytingsvik, Jenny Nikiforov, Vladimir Evenset, Anita Krøkje, Åse |
spellingShingle |
Neerland, Eirik D. Bytingsvik, Jenny Nikiforov, Vladimir Evenset, Anita Krøkje, Åse DNA double‐strand breaks in Arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
author_facet |
Neerland, Eirik D. Bytingsvik, Jenny Nikiforov, Vladimir Evenset, Anita Krøkje, Åse |
author_sort |
Neerland, Eirik D. |
title |
DNA double‐strand breaks in Arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_short |
DNA double‐strand breaks in Arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_full |
DNA double‐strand breaks in Arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_fullStr |
DNA double‐strand breaks in Arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_full_unstemmed |
DNA double‐strand breaks in Arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic |
title_sort |
dna double‐strand breaks in arctic char (salvelinus alpinus) from bjørnøya in the norwegian arctic |
publisher |
Wiley |
publishDate |
2019 |
url |
http://hdl.handle.net/11250/2637780 https://doi.org/10.1002/etc.4546 |
long_lat |
ENVELOPE(-67.250,-67.250,-68.151,-68.151) ENVELOPE(19.031,19.031,74.388,74.388) ENVELOPE(19.360,19.360,69.361,69.361) |
geographic |
Arctic Bjørnøya Ellasjøen Laksvatn |
geographic_facet |
Arctic Bjørnøya Ellasjøen Laksvatn |
genre |
Arctic Bjørnøya Bjørnøya Salvelinus alpinus |
genre_facet |
Arctic Bjørnøya Bjørnøya Salvelinus alpinus |
op_source |
2405-2413 38 Environmental Toxicology and Chemistry 11 |
op_relation |
Norges forskningsråd: 221371 Framsenteret: Flagship "Hazardous substances" Environmental Toxicology and Chemistry. 2019, 38 (11), 2405-2413. urn:issn:0730-7268 http://hdl.handle.net/11250/2637780 https://doi.org/10.1002/etc.4546 cristin:1735983 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1002/etc.4546 |
container_title |
Environmental Toxicology and Chemistry |
container_volume |
38 |
container_issue |
11 |
container_start_page |
2405 |
op_container_end_page |
2413 |
_version_ |
1766306500749295616 |