A systemic study of lipid metabolism regulation in salmon fingerlings and early juveniles fed plant oil
In salmon farming, the scarcity of fish oil has driven a shift towards the use of plant-based oil from vegetable or seed, leading to fish feed low in long-chain PUFA (LC-PUFA) and cholesterol. Atlantic salmon has the capacity to synthesise both LC-PUFA and cholesterol, but little is known about the...
Published in: | British Journal of Nutrition |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Cambridge University Press
2018
|
Subjects: | |
Online Access: | http://hdl.handle.net/11250/2583067 https://doi.org/10.1017/S0007114518001885 |
id |
ftntnutrondheimi:oai:ntnuopen.ntnu.no:11250/2583067 |
---|---|
record_format |
openpolar |
spelling |
ftntnutrondheimi:oai:ntnuopen.ntnu.no:11250/2583067 2023-05-15T15:32:49+02:00 A systemic study of lipid metabolism regulation in salmon fingerlings and early juveniles fed plant oil Jin, Yang Olsen, Rolf Erik Gillard, Gareth B. Østensen, Mari-Ann Korsvoll, Sven A. Santi, Nina Vik, Jon Olav Sandve, Simen Rød Olsen, Yngvar 2018 http://hdl.handle.net/11250/2583067 https://doi.org/10.1017/S0007114518001885 eng eng Cambridge University Press Norges forskningsråd: 244164 Norges forskningsråd: 248792 British Journal of Nutrition. 2018, 120 (6), 653-664. urn:issn:0007-1145 http://hdl.handle.net/11250/2583067 https://doi.org/10.1017/S0007114518001885 cristin:1607000 653-664 120 British Journal of Nutrition 6 Journal article Peer reviewed 2018 ftntnutrondheimi https://doi.org/10.1017/S0007114518001885 2019-09-17T06:54:25Z In salmon farming, the scarcity of fish oil has driven a shift towards the use of plant-based oil from vegetable or seed, leading to fish feed low in long-chain PUFA (LC-PUFA) and cholesterol. Atlantic salmon has the capacity to synthesise both LC-PUFA and cholesterol, but little is known about the regulation of synthesis and how it varies throughout salmon life span. Here, we present a systemic view of lipid metabolism pathways based on lipid analyses and transcriptomic data from salmon fed contrasting diets of plant or fish oil from first feeding. We analysed four tissues (stomach, pyloric caeca, hindgut and liver) at three life stages (initial feeding 0·16 g, 2·5 g fingerlings and 10 g juveniles). The strongest response to diets higher in plant oil was seen in pyloric caeca of fingerlings, with up-regulation of thirty genes in pathways for cholesterol uptake, transport and biosynthesis. In juveniles, only eleven genes showed differential expression in pyloric caeca. This indicates a higher requirement of dietary cholesterol in fingerlings, which could result in a more sensitive response to plant oil. The LC-PUFA elongation and desaturation pathway was down-regulated in pyloric caeca, probably regulated by srebp1 genes. In liver, cholesterol metabolism and elongation and desaturation genes were both higher on plant oil. Stomach and hindgut were not notably affected by dietary treatment. Plant oil also had a higher impact on fatty acid composition of fingerlings compared with juveniles, suggesting that fingerlings have less metabolic regulatory control when primed with plant oil diet compared with juveniles. publishedVersion © The Authors 2018 Article in Journal/Newspaper Atlantic salmon NTNU Open Archive (Norwegian University of Science and Technology) British Journal of Nutrition 120 6 653 664 |
institution |
Open Polar |
collection |
NTNU Open Archive (Norwegian University of Science and Technology) |
op_collection_id |
ftntnutrondheimi |
language |
English |
description |
In salmon farming, the scarcity of fish oil has driven a shift towards the use of plant-based oil from vegetable or seed, leading to fish feed low in long-chain PUFA (LC-PUFA) and cholesterol. Atlantic salmon has the capacity to synthesise both LC-PUFA and cholesterol, but little is known about the regulation of synthesis and how it varies throughout salmon life span. Here, we present a systemic view of lipid metabolism pathways based on lipid analyses and transcriptomic data from salmon fed contrasting diets of plant or fish oil from first feeding. We analysed four tissues (stomach, pyloric caeca, hindgut and liver) at three life stages (initial feeding 0·16 g, 2·5 g fingerlings and 10 g juveniles). The strongest response to diets higher in plant oil was seen in pyloric caeca of fingerlings, with up-regulation of thirty genes in pathways for cholesterol uptake, transport and biosynthesis. In juveniles, only eleven genes showed differential expression in pyloric caeca. This indicates a higher requirement of dietary cholesterol in fingerlings, which could result in a more sensitive response to plant oil. The LC-PUFA elongation and desaturation pathway was down-regulated in pyloric caeca, probably regulated by srebp1 genes. In liver, cholesterol metabolism and elongation and desaturation genes were both higher on plant oil. Stomach and hindgut were not notably affected by dietary treatment. Plant oil also had a higher impact on fatty acid composition of fingerlings compared with juveniles, suggesting that fingerlings have less metabolic regulatory control when primed with plant oil diet compared with juveniles. publishedVersion © The Authors 2018 |
format |
Article in Journal/Newspaper |
author |
Jin, Yang Olsen, Rolf Erik Gillard, Gareth B. Østensen, Mari-Ann Korsvoll, Sven A. Santi, Nina Vik, Jon Olav Sandve, Simen Rød Olsen, Yngvar |
spellingShingle |
Jin, Yang Olsen, Rolf Erik Gillard, Gareth B. Østensen, Mari-Ann Korsvoll, Sven A. Santi, Nina Vik, Jon Olav Sandve, Simen Rød Olsen, Yngvar A systemic study of lipid metabolism regulation in salmon fingerlings and early juveniles fed plant oil |
author_facet |
Jin, Yang Olsen, Rolf Erik Gillard, Gareth B. Østensen, Mari-Ann Korsvoll, Sven A. Santi, Nina Vik, Jon Olav Sandve, Simen Rød Olsen, Yngvar |
author_sort |
Jin, Yang |
title |
A systemic study of lipid metabolism regulation in salmon fingerlings and early juveniles fed plant oil |
title_short |
A systemic study of lipid metabolism regulation in salmon fingerlings and early juveniles fed plant oil |
title_full |
A systemic study of lipid metabolism regulation in salmon fingerlings and early juveniles fed plant oil |
title_fullStr |
A systemic study of lipid metabolism regulation in salmon fingerlings and early juveniles fed plant oil |
title_full_unstemmed |
A systemic study of lipid metabolism regulation in salmon fingerlings and early juveniles fed plant oil |
title_sort |
systemic study of lipid metabolism regulation in salmon fingerlings and early juveniles fed plant oil |
publisher |
Cambridge University Press |
publishDate |
2018 |
url |
http://hdl.handle.net/11250/2583067 https://doi.org/10.1017/S0007114518001885 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
653-664 120 British Journal of Nutrition 6 |
op_relation |
Norges forskningsråd: 244164 Norges forskningsråd: 248792 British Journal of Nutrition. 2018, 120 (6), 653-664. urn:issn:0007-1145 http://hdl.handle.net/11250/2583067 https://doi.org/10.1017/S0007114518001885 cristin:1607000 |
op_doi |
https://doi.org/10.1017/S0007114518001885 |
container_title |
British Journal of Nutrition |
container_volume |
120 |
container_issue |
6 |
container_start_page |
653 |
op_container_end_page |
664 |
_version_ |
1766363303165034496 |