The mechanism (Phe362Tyr mutation) behind resistance in Lepeophtheirus salmonis pre-dates organophosphate use in salmon farming
The salmon louse is an ectoparasitic copepod of salmonids in the marine environment, and represents a global challenge to salmon aquaculture. A major issue is the reliance of the industry on a limited number of chemicals to delouse salmonids on farms, and the high levels of resistance that lice have...
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ftntnutrondheimi:oai:ntnuopen.ntnu.no:11250/2460637 2023-05-15T17:29:00+02:00 The mechanism (Phe362Tyr mutation) behind resistance in Lepeophtheirus salmonis pre-dates organophosphate use in salmon farming Kaur, Kiranpreet Besnier, Francois Glover, Kevin Nilsen, Frank Aspehaug, Vidar Teis Fjørtoft, Helene Børretzen Horsberg, Tor Einar 2017 http://hdl.handle.net/11250/2460637 https://doi.org/10.1038/s41598-017-12384-6 eng eng Nature Publishing Group Scientific Reports. 2017, 7 . urn:issn:2045-2322 http://hdl.handle.net/11250/2460637 https://doi.org/10.1038/s41598-017-12384-6 cristin:1504908 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no CC-BY 8 7 Scientific Reports Journal article Peer reviewed 2017 ftntnutrondheimi https://doi.org/10.1038/s41598-017-12384-6 2019-09-17T06:53:00Z The salmon louse is an ectoparasitic copepod of salmonids in the marine environment, and represents a global challenge to salmon aquaculture. A major issue is the reliance of the industry on a limited number of chemicals to delouse salmonids on farms, and the high levels of resistance that lice have developed to all of these agents. However, for most of these chemicals, resistance and dispersal mechanisms are unknown. We recently demonstrated that the Phe362Tyr mutation is the primary cause of organophosphate resistance in lice collected on Norwegian farms. In the present study, we genotyped >2000 lice collected throughout the entire North Atlantic in the period 1998–2016, using Phe362Tyr and nine tightly linked SNPs. Our results showed that the Phe362Tyr mutation is strongly linked to lice survival following chemical treatment on farms located throughout the North Atlantic, demonstrating for the first time, that this mutation represents the primary mechanism for organophosphate resistance in salmon lice across the North Atlantic. Additionally, we observed multiple and diverse high frequency haplotypes linked with the allele conveying resistance to organophosphate. We, therefore, conclude that Phe362Tyr is not a de novo mutation, but probably existed in salmon lice before the introduction of organophosphates in commercial aquaculture. publishedVersion © 2017 The Authors. This is an Open Access article licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0) Article in Journal/Newspaper North Atlantic NTNU Open Archive (Norwegian University of Science and Technology) Scientific Reports 7 1 |
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NTNU Open Archive (Norwegian University of Science and Technology) |
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ftntnutrondheimi |
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English |
description |
The salmon louse is an ectoparasitic copepod of salmonids in the marine environment, and represents a global challenge to salmon aquaculture. A major issue is the reliance of the industry on a limited number of chemicals to delouse salmonids on farms, and the high levels of resistance that lice have developed to all of these agents. However, for most of these chemicals, resistance and dispersal mechanisms are unknown. We recently demonstrated that the Phe362Tyr mutation is the primary cause of organophosphate resistance in lice collected on Norwegian farms. In the present study, we genotyped >2000 lice collected throughout the entire North Atlantic in the period 1998–2016, using Phe362Tyr and nine tightly linked SNPs. Our results showed that the Phe362Tyr mutation is strongly linked to lice survival following chemical treatment on farms located throughout the North Atlantic, demonstrating for the first time, that this mutation represents the primary mechanism for organophosphate resistance in salmon lice across the North Atlantic. Additionally, we observed multiple and diverse high frequency haplotypes linked with the allele conveying resistance to organophosphate. We, therefore, conclude that Phe362Tyr is not a de novo mutation, but probably existed in salmon lice before the introduction of organophosphates in commercial aquaculture. publishedVersion © 2017 The Authors. This is an Open Access article licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0) |
format |
Article in Journal/Newspaper |
author |
Kaur, Kiranpreet Besnier, Francois Glover, Kevin Nilsen, Frank Aspehaug, Vidar Teis Fjørtoft, Helene Børretzen Horsberg, Tor Einar |
spellingShingle |
Kaur, Kiranpreet Besnier, Francois Glover, Kevin Nilsen, Frank Aspehaug, Vidar Teis Fjørtoft, Helene Børretzen Horsberg, Tor Einar The mechanism (Phe362Tyr mutation) behind resistance in Lepeophtheirus salmonis pre-dates organophosphate use in salmon farming |
author_facet |
Kaur, Kiranpreet Besnier, Francois Glover, Kevin Nilsen, Frank Aspehaug, Vidar Teis Fjørtoft, Helene Børretzen Horsberg, Tor Einar |
author_sort |
Kaur, Kiranpreet |
title |
The mechanism (Phe362Tyr mutation) behind resistance in Lepeophtheirus salmonis pre-dates organophosphate use in salmon farming |
title_short |
The mechanism (Phe362Tyr mutation) behind resistance in Lepeophtheirus salmonis pre-dates organophosphate use in salmon farming |
title_full |
The mechanism (Phe362Tyr mutation) behind resistance in Lepeophtheirus salmonis pre-dates organophosphate use in salmon farming |
title_fullStr |
The mechanism (Phe362Tyr mutation) behind resistance in Lepeophtheirus salmonis pre-dates organophosphate use in salmon farming |
title_full_unstemmed |
The mechanism (Phe362Tyr mutation) behind resistance in Lepeophtheirus salmonis pre-dates organophosphate use in salmon farming |
title_sort |
mechanism (phe362tyr mutation) behind resistance in lepeophtheirus salmonis pre-dates organophosphate use in salmon farming |
publisher |
Nature Publishing Group |
publishDate |
2017 |
url |
http://hdl.handle.net/11250/2460637 https://doi.org/10.1038/s41598-017-12384-6 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
8 7 Scientific Reports |
op_relation |
Scientific Reports. 2017, 7 . urn:issn:2045-2322 http://hdl.handle.net/11250/2460637 https://doi.org/10.1038/s41598-017-12384-6 cristin:1504908 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-017-12384-6 |
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Scientific Reports |
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7 |
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1 |
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1766122311070515200 |