以酵素法進行麴酸酯生產之製程開發

碩士國立清華大學化學工程學系 GH000923607 麴酸為絲狀真菌所代謝的有機酸，其具有特殊的生物性質，所以常被應用在許多用途上，例如止痛劑、食品添加劑。近年來更發現具有抑制黑色素生成能力，而被大量使用於美白保養品中。但是，麴酸是一種水溶性物質，而且對光和熱效應敏感，因此影響其安定性，這些原因都造成麴酸在應用上的限制。有鑒於麴酸廣泛的應用性，如何增加麴酸的脂溶性與安定性成為一個重要的課題。藉由脂肪酸與麴酸進行酯化反應產生的麴酸酯，已被證實是一種有效改質麴酸安定性並提高麴酸脂溶性之方法。本實驗利用酵素法進行麴酸酯的生產，此外，酵素反應具有較高選擇性與反應條件較溫和的優點，有利於反應後麴酸...

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Main Authors:	蔡忠廷, Tsai chung-ting
Other Authors:	吳文騰, Wu Wen-Teng
Language:	Chinese
Published:	2005
Subjects:	麴酸麴狻酯酯化反應脂肪酸分解酶尿素包埋法 24 antartic*
Online Access:	http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/31104

id	ftnthuniv:oai:nthur.lib.nthu.edu.tw:987654321/31104
record_format	openpolar
institution	Open Polar
collection	National Tsing Hua University Institutional Repository (NTHUR)
op_collection_id	ftnthuniv
language	Chinese
topic	麴酸麴狻酯酯化反應脂肪酸分解酶尿素包埋法 24
spellingShingle	麴酸麴狻酯酯化反應脂肪酸分解酶尿素包埋法 24 蔡忠廷 Tsai chung-ting 以酵素法進行麴酸酯生產之製程開發
topic_facet	麴酸麴狻酯酯化反應脂肪酸分解酶尿素包埋法 24
description	碩士國立清華大學化學工程學系 GH000923607 麴酸為絲狀真菌所代謝的有機酸，其具有特殊的生物性質，所以常被應用在許多用途上，例如止痛劑、食品添加劑。近年來更發現具有抑制黑色素生成能力，而被大量使用於美白保養品中。但是，麴酸是一種水溶性物質，而且對光和熱效應敏感，因此影響其安定性，這些原因都造成麴酸在應用上的限制。有鑒於麴酸廣泛的應用性，如何增加麴酸的脂溶性與安定性成為一個重要的課題。藉由脂肪酸與麴酸進行酯化反應產生的麴酸酯，已被證實是一種有效改質麴酸安定性並提高麴酸脂溶性之方法。本實驗利用酵素法進行麴酸酯的生產，此外，酵素反應具有較高選擇性與反應條件較溫和的優點，有利於反應後麴酸酯純化分離。本研究目標產物為麴酸棕櫚酯，利用固定化酵素Novozyme 435 ( Candida antartica , type B lipase ) 做為催化劑，並探討最佳酯化反應條件與麴酸酯純化方法。本研究在酯化反應條件的研究上，探討了Lipase種類、反應溫度、麴酸/棕櫚酸比例、和分子篩添加量對酯化反應的影響。最佳反應結果顯示1 mmole 麴酸與2 mmole棕櫚酸於15 ml 的丙酮溶液中，添加1.5 g Novozyme 435以及4 g分子篩於40℃中，反應30小時，其轉化率可達92%。在麴酸棕櫚酯的純化研究中，發現透過尿素包埋法來進行純化，其最佳純化結果顯示2 g的初步純化物與4 g尿素溶於40 ml甲醇溶液中，能得到純0.88的麴酸棕櫚酯。
author2	吳文騰 Wu Wen-Teng
author	蔡忠廷 Tsai chung-ting
author_facet	蔡忠廷 Tsai chung-ting
author_sort	蔡忠廷
title	以酵素法進行麴酸酯生產之製程開發
title_short	以酵素法進行麴酸酯生產之製程開發
title_full	以酵素法進行麴酸酯生產之製程開發
title_fullStr	以酵素法進行麴酸酯生產之製程開發
title_full_unstemmed	以酵素法進行麴酸酯生產之製程開發
title_sort	以酵素法進行麴酸酯生產之製程開發
publishDate	2005
url	http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/31104
genre	antartic*
genre_facet	antartic*
op_relation	參考文獻 Ahuja S.K., Ferreira G.M., Moreira A.R. Application of Plackett-Burman design and response surface methodology to achieve exponential growth for aggregated shipworm bacterium.Biotechnology and Bioengineering 85:666-675, 2004 Bajpai P., Agrawal P.K. and Vishwanathan L. Kojic Acid - Synthesis and Properties. Journal of Scientific and Industrial Research, 41:185-194, 1982a. Bajpai P., Agrawal P.K. and Vishwanathan L. Kojic Acid - Synthesis and Properties. Journal of Scientific and Industrial Research, 41:185-194, 1982b. Balaz S., Uher M., Brtko J., Veverka M., Bransova J., Dobias J., Podova M. and Buchvald J. Relationship between antifungal activity and hydrophobicity of kojic acid derivatives. Folia Microbiology, 38:387-391, 1993. Box G.E.P. and Wilson K.B. On the experimental attainment of optimum conditions. Journal of the Royal Statistical Society B 13:1-45, 1951. Beelik A. Kojic acid. Advances in Carbohydrate Chemistry 48:145-183, 1956. Bentley R. Preparation and analysis of kojic acid. Methods in Enzymology, 3:238-241, 1957 Budavari S., O'Neil M.J., Smith A. and Heckelman P.E. The Merck Index. 11th . Rahway, NJ, 1989 Burdock G.A., Soni M.G. and Carabin I.G. Evaluation of health aspects of kojic acid in food. Regulatory Toxicology and Pharmacology, 33:80-101, 2001. Cao L., Bornscheuer U. T. and Schmid R. D. Lipase-catalyzed solid-phase synthesis of sugar esters. Influence og immobilization on productivity and stability of the enzyme, Journal of Molecular Catalysis B: Enzymatic 6:279-285, 1999 Chen C.S., Liu K.J., Lou Y.H. and Shieh C.J. Optimisation of kojic acid monolaurate synthesis with lipase PS from Pseudomonas cepacia, Journal of the Science of Food and Agriculture, 82:601-605, 2002 Chen J.S., Wei C., Rolle R.S., Otwell W.S., Balaban M.O., and Marshall M.R. Inhibitory Effect of Kojic acid on Some Plant and Crustacean Polyphenol Oxidases, Journal of Agricultural and Food Chemistry 39:1396-1401, 1991a Chen J.S., Wei C. and Marshall M.R. Inhibition Mechanism of Kojic Acid on Polyphenol Oxidase, Ibid,39:1897-1901, 1991b Chen J.W. and Wu W.T., Regeneration of Immobilized Candida Antarctica Lipase for Transesterification, Journal of Bioscience and Bioengineering, 95:466-469, 2003 Chowdary G.V., Ramesh M.N. and Prapulla S.G. Enzymatic synthsis od isoamyl isovalerate using immobilized lipase from Rhizomucor miehei:multivariate analysis, Process Biochemistry,36:331-339, 2001 Ducret A. and Lortie R. Lipase catalysed enantioselective esterification of ibuprofen in organic solvent under controlled water activity. Enzyme and Microbial Technology 22:212-216, 1998 Ferreira-Dias S., Correia A.C., and da Fonseca M.M.R. Response surface modeling of glycerolysis catalyzed by Candida rugosa lipase immobilized in different polyurethane foams for the production of partial glycerides. Journal of Molecular Catalysis B: Enzymatic 21: 71-80, 2003 Gerhard J.,Kirkland D., Marzin D., Toutain H. and Jinnai H. An assessment of the genotoxicity and human health risk of topical use of kojic acid [5-hydroxy-2-(hydroxymethyl)-4H-pyran-4-one] . Food and Chemical Toxicology, 42:93-105, 2004 Gulten S., Sibel F. and Esra I. Production and Characterisation of enzymatically produced lauric acid esters of fructose, Journal of the Science of Food and Agriculture,82:1516-1522, 2002 Hari-Krishna S., Manohar B., Divakar S., Prapulla S.G., and Karanth N.G., Optimization of isoamyl acetate production by using immobilized lipase from Mucor miehei by respoe surfacr methodology, Enzyme and Microbial Technology. 26:131-136, 2000 Hamsaveni D.R., Prapulla S.G. and Divakar S. Response surface method-ological approach for the synthesis of isobutyl isobutyrate. Process Biochemistry.26:421-430, 2002 Hassan M.A., Ismail F., Yamamoto S., Yamada H. and Nakanishi K., Enzymatic synthesis of galactosyl kojic acid with immobilized-galactosidase from Bacillus circulans, Bioscience,Biotechnology, and Biochemistry, 59 : 543–545, 1995 He X.L., Chen B.Q. and Tan T.W., Enzymatic synthesis of 2-ethylhexyl esters of fatty acids by immobilized lipase from Candida sp. 99-125 Journal of Molecular Catalysis B: Enzymatic 18:333-339, 2002 Kayahara H., Shibata N., Tadasa K., Maeda H., Kotani T.and Ichimoto I., Amino acid and peptide derivatives of kojic acid and their antifungal properties, Agricultural and Biological Chemistry, 54:2441-2442, 1990 Kim M.G., and Lee S.B. Enzymatic Resolution of Racemic Ibuprofen by Lipase-Catalyzed Esterification Reaction: Effects of Water Content and Solid Supports, Journal of Fermentation and Bioengineering 3:269-271, 1995 Kim M.G. and Lee S.B. Enhanced catalytic efficiency of enzymes in organic media with the addition og the solid support: Effect of silica gel on reaction rates and enzyme agglomeration, Journal of Molecular Catalysis B: Enzymatic 2:127-140, 1996 Kiran K.R. and Divakar S.A. Central composite rotatable design analysis of lipase catalyzed synthesis of lauroyl lactic acid at bench-scale level. Enzyme and Microbial Technology 29:122-128, 2001 Kitao S. and Sekine H. Synthesis of two kojic acid glucosides with sucrose phosphorylase from Leuconostoc mesenteroides. Bioscience,Biotechnology, and Biochemistry, 58:419–420, 1994 Kobayashi T., Adachi S., Nakanishi K. and Matsuno R. Semi-continuous production of lauroyl kojic acid through lipase-catalyzed condensation in acetonitrile, Biochemical Engineering Journal, 9:85–89, 2001 Liu K.J. and Shaw J.F. Lipase-Catalyzed syntheses of kojic acid ester in organic solvent, Journal of the American Oil Chemists' Society,75:1507-1511, 1998 Marwaha S.S., Kaur J. and Sodhi G.S. Organomercury(II) complexes of kojic acid and maltol: synthesis, characterization, and biological studies. Journal of Inorganic Biochemistry 54:67-74, 1994. Matinelle M., Holmquist M. and Hult K. Onthe interfacial activation of Candida antartica lipase A and B as compared with Humicola lanuginose lipase. Biochimica et Biophysica Acta. International Journal of Biochemistry and Biophysics 1250:272-276, 1995 Medina A.R,P’aez B.C.,Rubio F.C., Moreno P.G. and Groma E.M. Modeling the effect of free water on enzyme activity in immobilized lipase-catalyzed reactions in organic solvents, Enzyme and Microbial Technology 33:845-853, 2003 Nakajima N., Ishihara K. and Hamada H. Functional Glucosylation of Kojic Acid and Daidzein with the Eucalyptus Membrane-Associated UDP-Glucosyltransferase Reaction Systems. Journal of Bioscience and Bioengineer, 92: 469-471, 2001 Nelson L.A., Fogila T.A. and Marmer W.N. Lipase-catalyzed production of biodiesel, Journal of the American Oil Chemists' Society 73:1191-1195, 1996 Nishimura T., Kometani T., Takii H., Terada Y. and Okada S. Acceptor specificity in the glucosylation reaction of Bacillus subtilis X-23-amylase towards various phenolic compounds and the structure of kojic acid glucoside, Journal of Fermentation and Bioengineering, 78: 37–41, 1994 Raku T. and Tokiwa Y. Regioselective synthesis of kojic acid esters by Bacillus subtilis protease, Biotechnology Letters 25:969-974, 2003 Saruno R., Kato F. and Ikeno T. Kojic acid,a Tyrosinase Inhibitor from Aspergillus albus, Agricultural and Biological Chemistry, 43:1337-1338, 1979 Selmi B., Gontier E., Ergan F.,Barbotin J.N. and Thomas D. Lipase-catalyzed synthesis of tricaprylin in a medium solely composed of substrstes. Water production and elimination, Enzyme and Microbial Technology 20:322-325, 1997 Tao R.B. and Huiliang W.J. Preparation and property of kojic dipalmitate, Riyong Huaxue Gongye, 33:124-126, 2003 Traitler H.,Wille H.J. and Studer A. Fractionation of blackcurrant seed oil , Journal of the American Oil Chemists' Society, 65:755-760, 1988 Tweddell R.J., Kermasha S., Combes D. and Marty A. Esterification and interesterification activities of lipases from Rhizopus niveus and Mucor miehei in three different types of organic media:a comparative study, Enzyme and Microbial Technology 22:439-445, 1998 Watanabe Y.,Shimada Y., Sugihara A., Honda H., Fukuda H. and Tominaga Y. Continuous production of biodiesel fuel from vegetable oil using immobilized Candida Antarctica lipase, Journal of the American Oil Chemists' Society 77:355-360, 2000 Wille H.J., Trartler H. and Kelly M. Production of polyenoic fish oil fatty acids by combined urea fractionation and industrial scale preparative HPLC,Revue Francaise des corps gras,34:69-73, 1987 Won K. and Lee S.B. Computer-Aided Control of Water Activity for Lipase-Catalyzed Esterification in Solvent-free Systems, Biotechnology Progress 17:258-264, 2001 Zhang L.Q., Zhang Y.D, Xu L,Li X.L, Yang X.C, Xu G.L, Wu X.X, Gao H.Y, Du W.B, Zhang X.T and Zhang X.Z. Lipase-catalyzed synthesis of RGD diamide in aqueous water-miscible organic solvents. Enzyme and Microbial Technology 29:129-35, 2001 李仰川, 化妝品原理, 1999, 文京圖書有限公司
_version_	1766287926904225792
spelling	ftnthuniv:oai:nthur.lib.nthu.edu.tw:987654321/31104 2023-05-15T14:15:35+02:00 以酵素法進行麴酸酯生產之製程開發 Process development of kojic acid ester production by using enzyme 蔡忠廷 Tsai chung-ting 吳文騰 Wu Wen-Teng 2005 155 bytes text/html http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/31104 zh_TW chi 參考文獻 Ahuja S.K., Ferreira G.M., Moreira A.R. Application of Plackett-Burman design and response surface methodology to achieve exponential growth for aggregated shipworm bacterium.Biotechnology and Bioengineering 85:666-675, 2004 Bajpai P., Agrawal P.K. and Vishwanathan L. Kojic Acid - Synthesis and Properties. Journal of Scientific and Industrial Research, 41:185-194, 1982a. Bajpai P., Agrawal P.K. and Vishwanathan L. Kojic Acid - Synthesis and Properties. Journal of Scientific and Industrial Research, 41:185-194, 1982b. Balaz S., Uher M., Brtko J., Veverka M., Bransova J., Dobias J., Podova M. and Buchvald J. Relationship between antifungal activity and hydrophobicity of kojic acid derivatives. Folia Microbiology, 38:387-391, 1993. Box G.E.P. and Wilson K.B. On the experimental attainment of optimum conditions. Journal of the Royal Statistical Society B 13:1-45, 1951. Beelik A. Kojic acid. Advances in Carbohydrate Chemistry 48:145-183, 1956. Bentley R. Preparation and analysis of kojic acid. Methods in Enzymology, 3:238-241, 1957 Budavari S., O'Neil M.J., Smith A. and Heckelman P.E. The Merck Index. 11th . Rahway, NJ, 1989 Burdock G.A., Soni M.G. and Carabin I.G. Evaluation of health aspects of kojic acid in food. Regulatory Toxicology and Pharmacology, 33:80-101, 2001. Cao L., Bornscheuer U. T. and Schmid R. D. Lipase-catalyzed solid-phase synthesis of sugar esters. Influence og immobilization on productivity and stability of the enzyme, Journal of Molecular Catalysis B: Enzymatic 6:279-285, 1999 Chen C.S., Liu K.J., Lou Y.H. and Shieh C.J. Optimisation of kojic acid monolaurate synthesis with lipase PS from Pseudomonas cepacia, Journal of the Science of Food and Agriculture, 82:601-605, 2002 Chen J.S., Wei C., Rolle R.S., Otwell W.S., Balaban M.O., and Marshall M.R. Inhibitory Effect of Kojic acid on Some Plant and Crustacean Polyphenol Oxidases, Journal of Agricultural and Food Chemistry 39:1396-1401, 1991a Chen J.S., Wei C. and Marshall M.R. Inhibition Mechanism of Kojic Acid on Polyphenol Oxidase, Ibid,39:1897-1901, 1991b Chen J.W. and Wu W.T., Regeneration of Immobilized Candida Antarctica Lipase for Transesterification, Journal of Bioscience and Bioengineering, 95:466-469, 2003 Chowdary G.V., Ramesh M.N. and Prapulla S.G. Enzymatic synthsis od isoamyl isovalerate using immobilized lipase from Rhizomucor miehei:multivariate analysis, Process Biochemistry,36:331-339, 2001 Ducret A. and Lortie R. Lipase catalysed enantioselective esterification of ibuprofen in organic solvent under controlled water activity. Enzyme and Microbial Technology 22:212-216, 1998 Ferreira-Dias S., Correia A.C., and da Fonseca M.M.R. Response surface modeling of glycerolysis catalyzed by Candida rugosa lipase immobilized in different polyurethane foams for the production of partial glycerides. Journal of Molecular Catalysis B: Enzymatic 21: 71-80, 2003 Gerhard J.,Kirkland D., Marzin D., Toutain H. and Jinnai H. An assessment of the genotoxicity and human health risk of topical use of kojic acid [5-hydroxy-2-(hydroxymethyl)-4H-pyran-4-one] . Food and Chemical Toxicology, 42:93-105, 2004 Gulten S., Sibel F. and Esra I. Production and Characterisation of enzymatically produced lauric acid esters of fructose, Journal of the Science of Food and Agriculture,82:1516-1522, 2002 Hari-Krishna S., Manohar B., Divakar S., Prapulla S.G., and Karanth N.G., Optimization of isoamyl acetate production by using immobilized lipase from Mucor miehei by respoe surfacr methodology, Enzyme and Microbial Technology. 26:131-136, 2000 Hamsaveni D.R., Prapulla S.G. and Divakar S. Response surface method-ological approach for the synthesis of isobutyl isobutyrate. Process Biochemistry.26:421-430, 2002 Hassan M.A., Ismail F., Yamamoto S., Yamada H. and Nakanishi K., Enzymatic synthesis of galactosyl kojic acid with immobilized-galactosidase from Bacillus circulans, Bioscience,Biotechnology, and Biochemistry, 59 : 543–545, 1995 He X.L., Chen B.Q. and Tan T.W., Enzymatic synthesis of 2-ethylhexyl esters of fatty acids by immobilized lipase from Candida sp. 99-125 Journal of Molecular Catalysis B: Enzymatic 18:333-339, 2002 Kayahara H., Shibata N., Tadasa K., Maeda H., Kotani T.and Ichimoto I., Amino acid and peptide derivatives of kojic acid and their antifungal properties, Agricultural and Biological Chemistry, 54:2441-2442, 1990 Kim M.G., and Lee S.B. Enzymatic Resolution of Racemic Ibuprofen by Lipase-Catalyzed Esterification Reaction: Effects of Water Content and Solid Supports, Journal of Fermentation and Bioengineering 3:269-271, 1995 Kim M.G. and Lee S.B. Enhanced catalytic efficiency of enzymes in organic media with the addition og the solid support: Effect of silica gel on reaction rates and enzyme agglomeration, Journal of Molecular Catalysis B: Enzymatic 2:127-140, 1996 Kiran K.R. and Divakar S.A. Central composite rotatable design analysis of lipase catalyzed synthesis of lauroyl lactic acid at bench-scale level. Enzyme and Microbial Technology 29:122-128, 2001 Kitao S. and Sekine H. Synthesis of two kojic acid glucosides with sucrose phosphorylase from Leuconostoc mesenteroides. Bioscience,Biotechnology, and Biochemistry, 58:419–420, 1994 Kobayashi T., Adachi S., Nakanishi K. and Matsuno R. Semi-continuous production of lauroyl kojic acid through lipase-catalyzed condensation in acetonitrile, Biochemical Engineering Journal, 9:85–89, 2001 Liu K.J. and Shaw J.F. Lipase-Catalyzed syntheses of kojic acid ester in organic solvent, Journal of the American Oil Chemists' Society,75:1507-1511, 1998 Marwaha S.S., Kaur J. and Sodhi G.S. Organomercury(II) complexes of kojic acid and maltol: synthesis, characterization, and biological studies. Journal of Inorganic Biochemistry 54:67-74, 1994. Matinelle M., Holmquist M. and Hult K. Onthe interfacial activation of Candida antartica lipase A and B as compared with Humicola lanuginose lipase. Biochimica et Biophysica Acta. International Journal of Biochemistry and Biophysics 1250:272-276, 1995 Medina A.R,P’aez B.C.,Rubio F.C., Moreno P.G. and Groma E.M. Modeling the effect of free water on enzyme activity in immobilized lipase-catalyzed reactions in organic solvents, Enzyme and Microbial Technology 33:845-853, 2003 Nakajima N., Ishihara K. and Hamada H. Functional Glucosylation of Kojic Acid and Daidzein with the Eucalyptus Membrane-Associated UDP-Glucosyltransferase Reaction Systems. Journal of Bioscience and Bioengineer, 92: 469-471, 2001 Nelson L.A., Fogila T.A. and Marmer W.N. Lipase-catalyzed production of biodiesel, Journal of the American Oil Chemists' Society 73:1191-1195, 1996 Nishimura T., Kometani T., Takii H., Terada Y. and Okada S. Acceptor specificity in the glucosylation reaction of Bacillus subtilis X-23-amylase towards various phenolic compounds and the structure of kojic acid glucoside, Journal of Fermentation and Bioengineering, 78: 37–41, 1994 Raku T. and Tokiwa Y. Regioselective synthesis of kojic acid esters by Bacillus subtilis protease, Biotechnology Letters 25:969-974, 2003 Saruno R., Kato F. and Ikeno T. Kojic acid,a Tyrosinase Inhibitor from Aspergillus albus, Agricultural and Biological Chemistry, 43:1337-1338, 1979 Selmi B., Gontier E., Ergan F.,Barbotin J.N. and Thomas D. Lipase-catalyzed synthesis of tricaprylin in a medium solely composed of substrstes. Water production and elimination, Enzyme and Microbial Technology 20:322-325, 1997 Tao R.B. and Huiliang W.J. Preparation and property of kojic dipalmitate, Riyong Huaxue Gongye, 33:124-126, 2003 Traitler H.,Wille H.J. and Studer A. Fractionation of blackcurrant seed oil , Journal of the American Oil Chemists' Society, 65:755-760, 1988 Tweddell R.J., Kermasha S., Combes D. and Marty A. Esterification and interesterification activities of lipases from Rhizopus niveus and Mucor miehei in three different types of organic media:a comparative study, Enzyme and Microbial Technology 22:439-445, 1998 Watanabe Y.,Shimada Y., Sugihara A., Honda H., Fukuda H. and Tominaga Y. Continuous production of biodiesel fuel from vegetable oil using immobilized Candida Antarctica lipase, Journal of the American Oil Chemists' Society 77:355-360, 2000 Wille H.J., Trartler H. and Kelly M. Production of polyenoic fish oil fatty acids by combined urea fractionation and industrial scale preparative HPLC,Revue Francaise des corps gras,34:69-73, 1987 Won K. and Lee S.B. Computer-Aided Control of Water Activity for Lipase-Catalyzed Esterification in Solvent-free Systems, Biotechnology Progress 17:258-264, 2001 Zhang L.Q., Zhang Y.D, Xu L,Li X.L, Yang X.C, Xu G.L, Wu X.X, Gao H.Y, Du W.B, Zhang X.T and Zhang X.Z. Lipase-catalyzed synthesis of RGD diamide in aqueous water-miscible organic solvents. Enzyme and Microbial Technology 29:129-35, 2001 李仰川, 化妝品原理, 1999, 文京圖書有限公司麴酸麴狻酯酯化反應脂肪酸分解酶尿素包埋法 24 2005 ftnthuniv 2014-12-17T20:06:24Z 碩士國立清華大學化學工程學系 GH000923607 麴酸為絲狀真菌所代謝的有機酸，其具有特殊的生物性質，所以常被應用在許多用途上，例如止痛劑、食品添加劑。近年來更發現具有抑制黑色素生成能力，而被大量使用於美白保養品中。但是，麴酸是一種水溶性物質，而且對光和熱效應敏感，因此影響其安定性，這些原因都造成麴酸在應用上的限制。有鑒於麴酸廣泛的應用性，如何增加麴酸的脂溶性與安定性成為一個重要的課題。藉由脂肪酸與麴酸進行酯化反應產生的麴酸酯，已被證實是一種有效改質麴酸安定性並提高麴酸脂溶性之方法。本實驗利用酵素法進行麴酸酯的生產，此外，酵素反應具有較高選擇性與反應條件較溫和的優點，有利於反應後麴酸酯純化分離。本研究目標產物為麴酸棕櫚酯，利用固定化酵素Novozyme 435 ( Candida antartica , type B lipase ) 做為催化劑，並探討最佳酯化反應條件與麴酸酯純化方法。本研究在酯化反應條件的研究上，探討了Lipase種類、反應溫度、麴酸/棕櫚酸比例、和分子篩添加量對酯化反應的影響。最佳反應結果顯示1 mmole 麴酸與2 mmole棕櫚酸於15 ml 的丙酮溶液中，添加1.5 g Novozyme 435以及4 g分子篩於40℃中，反應30小時，其轉化率可達92%。在麴酸棕櫚酯的純化研究中，發現透過尿素包埋法來進行純化，其最佳純化結果顯示2 g的初步純化物與4 g尿素溶於40 ml甲醇溶液中，能得到純0.88的麴酸棕櫚酯。 Other/Unknown Material antartic* National Tsing Hua University Institutional Repository (NTHUR)

以酵素法進行麴酸酯生產之製程開發

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