Nucleotide Sequences and Functions of the Epstein-Barr Virus Latent Membrane Protein 1 Genes Isolated from Salivary Gland Lymphoepithelial Carcinomas
Epstein-Barr virus (EBV) infection is associated with salivary gland lymphoepithelial carcinoma (SLEC) and nasopharyngeal carcinoma (NPC). EBV is a ubiquitous herpes virus world wide, but EBV-associated SLEC and NPC are prevalent in restricted regions such as south areas of China , Southeastern Asia...
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ftntaiwanuniv:oai:140.112.114.62:246246/94003 2023-05-15T16:07:40+02:00 Nucleotide Sequences and Functions of the Epstein-Barr Virus Latent Membrane Protein 1 Genes Isolated from Salivary Gland Lymphoepithelial Carcinomas 陳志榮 CHEN, CHI-LONG 病理學科所 2005 http://ntur.lib.ntu.edu.tw/handle/246246/94003 en-us en_US eng VIRUS GENES v.30 n.2 pp.223 - 235 mutation;inhibition of proliferation;EBV;LMP1;salivary gland lymphoepithelial carcinoma (SLEC) 2005 ftntaiwanuniv 2016-02-20T00:59:27Z Epstein-Barr virus (EBV) infection is associated with salivary gland lymphoepithelial carcinoma (SLEC) and nasopharyngeal carcinoma (NPC). EBV is a ubiquitous herpes virus world wide, but EBV-associated SLEC and NPC are prevalent in restricted regions such as south areas of China , Southeastern Asia and Greenland (Eskimos). To examine whether particular EBV variants play roles in the development of SLEC and NPC, we isolated the complete EBV LMP1 genes from 12 paraffin-embedded biopsy samples of SLECs isolated from China, Taiwan and Russia, and compared these LMP1 genes with those of NPC (CAO) and the prototype B95-8 EBV. Nucleotide sequence analysis showed that SLECs LMP1 is more similar to that of CAO than that of prototype B95-8. The analysis also identified several conserved (67–100%) variations in SLEC-LMP1 and CAO-LMP1 distinct from B 95-8-LMP 1. These included 10-amino acid deletion, 5-amino acid deletion and 12-single amino acid variations. A SLEC-LMP1 gene with the aforementioned conserved variations inhibited the growth of an embryonic kidney cell line (293T), highly activated the NF-B pathway, and these activities were equivalent to those of B95-8 and CAO. These findings suggest that the biological functions of SLEC-LMP 1 are similar to those of B95-8-LMP1 and CAO-LMP1, and that these amino acid variations including the well-known 10-aa deletion did not affect these two prominent activities. While the present results could not uncover functional differences between SLEC-LMP1 and B95-8-LMP1, the nucleotide sequences and the molecular clone of LMP1 directly isolated from SLEC patients will be a useful tool to identify the high-pathogenic EBV strain(s), associated with SLEC and NPC. Other/Unknown Material eskimo* Greenland National Taiwan University Institutional Repository (NTUR) Greenland |
institution |
Open Polar |
collection |
National Taiwan University Institutional Repository (NTUR) |
op_collection_id |
ftntaiwanuniv |
language |
English |
topic |
mutation;inhibition of proliferation;EBV;LMP1;salivary gland lymphoepithelial carcinoma (SLEC) |
spellingShingle |
mutation;inhibition of proliferation;EBV;LMP1;salivary gland lymphoepithelial carcinoma (SLEC) 陳志榮 CHEN, CHI-LONG Nucleotide Sequences and Functions of the Epstein-Barr Virus Latent Membrane Protein 1 Genes Isolated from Salivary Gland Lymphoepithelial Carcinomas |
topic_facet |
mutation;inhibition of proliferation;EBV;LMP1;salivary gland lymphoepithelial carcinoma (SLEC) |
description |
Epstein-Barr virus (EBV) infection is associated with salivary gland lymphoepithelial carcinoma (SLEC) and nasopharyngeal carcinoma (NPC). EBV is a ubiquitous herpes virus world wide, but EBV-associated SLEC and NPC are prevalent in restricted regions such as south areas of China , Southeastern Asia and Greenland (Eskimos). To examine whether particular EBV variants play roles in the development of SLEC and NPC, we isolated the complete EBV LMP1 genes from 12 paraffin-embedded biopsy samples of SLECs isolated from China, Taiwan and Russia, and compared these LMP1 genes with those of NPC (CAO) and the prototype B95-8 EBV. Nucleotide sequence analysis showed that SLECs LMP1 is more similar to that of CAO than that of prototype B95-8. The analysis also identified several conserved (67–100%) variations in SLEC-LMP1 and CAO-LMP1 distinct from B 95-8-LMP 1. These included 10-amino acid deletion, 5-amino acid deletion and 12-single amino acid variations. A SLEC-LMP1 gene with the aforementioned conserved variations inhibited the growth of an embryonic kidney cell line (293T), highly activated the NF-B pathway, and these activities were equivalent to those of B95-8 and CAO. These findings suggest that the biological functions of SLEC-LMP 1 are similar to those of B95-8-LMP1 and CAO-LMP1, and that these amino acid variations including the well-known 10-aa deletion did not affect these two prominent activities. While the present results could not uncover functional differences between SLEC-LMP1 and B95-8-LMP1, the nucleotide sequences and the molecular clone of LMP1 directly isolated from SLEC patients will be a useful tool to identify the high-pathogenic EBV strain(s), associated with SLEC and NPC. |
author2 |
病理學科所 |
author |
陳志榮 CHEN, CHI-LONG |
author_facet |
陳志榮 CHEN, CHI-LONG |
author_sort |
陳志榮 |
title |
Nucleotide Sequences and Functions of the Epstein-Barr Virus Latent Membrane Protein 1 Genes Isolated from Salivary Gland Lymphoepithelial Carcinomas |
title_short |
Nucleotide Sequences and Functions of the Epstein-Barr Virus Latent Membrane Protein 1 Genes Isolated from Salivary Gland Lymphoepithelial Carcinomas |
title_full |
Nucleotide Sequences and Functions of the Epstein-Barr Virus Latent Membrane Protein 1 Genes Isolated from Salivary Gland Lymphoepithelial Carcinomas |
title_fullStr |
Nucleotide Sequences and Functions of the Epstein-Barr Virus Latent Membrane Protein 1 Genes Isolated from Salivary Gland Lymphoepithelial Carcinomas |
title_full_unstemmed |
Nucleotide Sequences and Functions of the Epstein-Barr Virus Latent Membrane Protein 1 Genes Isolated from Salivary Gland Lymphoepithelial Carcinomas |
title_sort |
nucleotide sequences and functions of the epstein-barr virus latent membrane protein 1 genes isolated from salivary gland lymphoepithelial carcinomas |
publishDate |
2005 |
url |
http://ntur.lib.ntu.edu.tw/handle/246246/94003 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
eskimo* Greenland |
genre_facet |
eskimo* Greenland |
op_relation |
VIRUS GENES v.30 n.2 pp.223 - 235 |
_version_ |
1766403770523058176 |