Characterization of the Metabolic Actions of Crude Versus Dispersed Oil in Salmon Smolts Via Nmr-Based Metabolomics
With maritime transport of crude oil from Alaska to California, there is significant potential for a catastrophic spill which could impact migrating salmon. Therefore, this study compared the lethal and sublethal metabolic actions of the water-accommodated fraction (WAF) and the chemically enhanced...
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ftntaiwanuniv:oai:140.112.114.62:246246/235809 2023-05-15T18:03:38+02:00 Characterization of the Metabolic Actions of Crude Versus Dispersed Oil in Salmon Smolts Via Nmr-Based Metabolomics 林靖愉;吳宏達 LIN, CHING-YU;WU, HONG-DAR ISAAC 環境衛生研究所 2009 http://ntur.lib.ntu.edu.tw/handle/246246/235809 en-us en_US eng AQUATIC TOXICOLOGY v.95 n.3 pp.230-238 NMR; Metabolomics; Metabonomics; Chinook salmon; Onchorhyncus tshawytscha; Prudhoe Bay crude oil; Corexit 950 Toxicity; Oil spill article 2009 ftntaiwanuniv 2016-02-20T04:29:49Z With maritime transport of crude oil from Alaska to California, there is significant potential for a catastrophic spill which could impact migrating salmon. Therefore, this study compared the lethal and sublethal metabolic actions of the water-accommodated fraction (WAF) and the chemically enhanced WAF (CEWAF, via Corexit 9500) of Prudhoe Bay crude oil in smolts of Chinook salmon ( Onchorhyncus tshawytscha). After 96-h exposure to the CEWAF, the resulting LC50 was some 20 times higher (i.e., less toxic) than that of the WAR Muscle and liver samples from surviving fish were collected and low-molecular weight metabolites were analyzed using one-dimensional H-1 and projections of two-dimensional H-1 J- resolved NMR. Principal component analysis (PCA), employed to analyze NMR spectra and identify most variance from the samples, revealed age- related metabolic changes in the fish within the replicated studies, but few consistent metabolic effects from the treatments. However, ANOVA results demonstrated that the dose-response metabolite patterns are both metabolite- and organ-dependent. In general, exposure to either WAF or CEWAF resulted in an increase of amino acids (i.e., valine, glutamine and glutamate) and a decrease of both organic osmolytes (i.e., glycerophosphorylcholine) and energetic substrates (i.e., succinate). The simultaneous increase of formate and decrease of glycerophosphorylcholine in the liver, or the decrease of glycerophosphorylcholine in muscle , may serve as sensitive sublethal biomarkers for WAF or CEWAF exposures. respectively. In conclusion, dispersant treatment significantly decreased the lethal potency of crude oil to salmon smolts. and the NMR-based metabolomics approach provided a sensitive means to characterize the sublethal metabolic actions. Article in Journal/Newspaper Prudhoe Bay Alaska National Taiwan University Institutional Repository (NTUR) |
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Open Polar |
collection |
National Taiwan University Institutional Repository (NTUR) |
op_collection_id |
ftntaiwanuniv |
language |
English |
topic |
NMR; Metabolomics; Metabonomics; Chinook salmon; Onchorhyncus tshawytscha; Prudhoe Bay crude oil; Corexit 950 Toxicity; Oil spill |
spellingShingle |
NMR; Metabolomics; Metabonomics; Chinook salmon; Onchorhyncus tshawytscha; Prudhoe Bay crude oil; Corexit 950 Toxicity; Oil spill 林靖愉;吳宏達 LIN, CHING-YU;WU, HONG-DAR ISAAC Characterization of the Metabolic Actions of Crude Versus Dispersed Oil in Salmon Smolts Via Nmr-Based Metabolomics |
topic_facet |
NMR; Metabolomics; Metabonomics; Chinook salmon; Onchorhyncus tshawytscha; Prudhoe Bay crude oil; Corexit 950 Toxicity; Oil spill |
description |
With maritime transport of crude oil from Alaska to California, there is significant potential for a catastrophic spill which could impact migrating salmon. Therefore, this study compared the lethal and sublethal metabolic actions of the water-accommodated fraction (WAF) and the chemically enhanced WAF (CEWAF, via Corexit 9500) of Prudhoe Bay crude oil in smolts of Chinook salmon ( Onchorhyncus tshawytscha). After 96-h exposure to the CEWAF, the resulting LC50 was some 20 times higher (i.e., less toxic) than that of the WAR Muscle and liver samples from surviving fish were collected and low-molecular weight metabolites were analyzed using one-dimensional H-1 and projections of two-dimensional H-1 J- resolved NMR. Principal component analysis (PCA), employed to analyze NMR spectra and identify most variance from the samples, revealed age- related metabolic changes in the fish within the replicated studies, but few consistent metabolic effects from the treatments. However, ANOVA results demonstrated that the dose-response metabolite patterns are both metabolite- and organ-dependent. In general, exposure to either WAF or CEWAF resulted in an increase of amino acids (i.e., valine, glutamine and glutamate) and a decrease of both organic osmolytes (i.e., glycerophosphorylcholine) and energetic substrates (i.e., succinate). The simultaneous increase of formate and decrease of glycerophosphorylcholine in the liver, or the decrease of glycerophosphorylcholine in muscle , may serve as sensitive sublethal biomarkers for WAF or CEWAF exposures. respectively. In conclusion, dispersant treatment significantly decreased the lethal potency of crude oil to salmon smolts. and the NMR-based metabolomics approach provided a sensitive means to characterize the sublethal metabolic actions. |
author2 |
環境衛生研究所 |
format |
Article in Journal/Newspaper |
author |
林靖愉;吳宏達 LIN, CHING-YU;WU, HONG-DAR ISAAC |
author_facet |
林靖愉;吳宏達 LIN, CHING-YU;WU, HONG-DAR ISAAC |
author_sort |
林靖愉;吳宏達 |
title |
Characterization of the Metabolic Actions of Crude Versus Dispersed Oil in Salmon Smolts Via Nmr-Based Metabolomics |
title_short |
Characterization of the Metabolic Actions of Crude Versus Dispersed Oil in Salmon Smolts Via Nmr-Based Metabolomics |
title_full |
Characterization of the Metabolic Actions of Crude Versus Dispersed Oil in Salmon Smolts Via Nmr-Based Metabolomics |
title_fullStr |
Characterization of the Metabolic Actions of Crude Versus Dispersed Oil in Salmon Smolts Via Nmr-Based Metabolomics |
title_full_unstemmed |
Characterization of the Metabolic Actions of Crude Versus Dispersed Oil in Salmon Smolts Via Nmr-Based Metabolomics |
title_sort |
characterization of the metabolic actions of crude versus dispersed oil in salmon smolts via nmr-based metabolomics |
publishDate |
2009 |
url |
http://ntur.lib.ntu.edu.tw/handle/246246/235809 |
genre |
Prudhoe Bay Alaska |
genre_facet |
Prudhoe Bay Alaska |
op_relation |
AQUATIC TOXICOLOGY v.95 n.3 pp.230-238 |
_version_ |
1766174556238643200 |