A “Rosetta Stone” for Metazoan Zooplankton: DNA Barcode Analysis of Species Diversity of the Sargasso Sea (Northwest Atlantic Ocean)
Species diversity of the metazoan holozooplankton assemblage of the Sargasso Sea, Northwest Atlantic Ocean, was examined through coordinated morphological taxonomic identification of species and DNA sequencing of a ∼650 base-pair region of mitochondrial cytochrome oxidase I (mtCOI) as a DNA barcode...
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ftnsoutheastern:oai:nsuworks.nova.edu:occ_facarticles-1525 2023-05-15T17:45:36+02:00 A “Rosetta Stone” for Metazoan Zooplankton: DNA Barcode Analysis of Species Diversity of the Sargasso Sea (Northwest Atlantic Ocean) Bucklin, Ann Ortman, Brian D. Jennings, Robert M. Nigro, Lisa M. Sweetman, Christopher J. Copley, Nancy J. Sutton, Tracey Wiebe, Peter 2010-12-01T08:00:00Z https://nsuworks.nova.edu/occ_facarticles/517 unknown NSUWorks https://nsuworks.nova.edu/occ_facarticles/517 Marine & Environmental Sciences Faculty Articles Zooplankton DNA barcode Sargasso Sea Species diversity Deep sea Marine Biology Oceanography and Atmospheric Sciences and Meteorology article 2010 ftnsoutheastern 2022-04-10T21:29:44Z Species diversity of the metazoan holozooplankton assemblage of the Sargasso Sea, Northwest Atlantic Ocean, was examined through coordinated morphological taxonomic identification of species and DNA sequencing of a ∼650 base-pair region of mitochondrial cytochrome oxidase I (mtCOI) as a DNA barcode (i.e., short sequence for species recognition and discrimination). Zooplankton collections were made from the surface to 5,000 meters during April, 2006 on the R/V R.H. Brown. Samples were examined by a ship-board team of morphological taxonomists; DNA barcoding was carried out in both ship-board and land-based DNA sequencing laboratories. DNA barcodes were determined for a total of 297 individuals of 175 holozooplankton species in four phyla, including: Cnidaria (Hydromedusae, 4 species; Siphonophora, 47); Arthropoda (Amphipoda, 10; Copepoda, 34; Decapoda, 9; Euphausiacea, 10; Mysidacea, 1; Ostracoda, 27); and Mollusca (Cephalopoda, 8; Heteropoda, 6; Pteropoda, 15); and Chaetognatha (4). Thirty species of fish (Teleostei) were also barcoded. For all seven zooplankton groups for which sufficient data were available, Kimura-2-Parameter genetic distances were significantly lower between individuals of the same species (mean=0.0114; S.D. 0.0117) than between individuals of different species within the same group (mean=0.3166; S.D. 0.0378). This difference, known as the barcode gap, ensures that mtCOI sequences are reliable characters for species identification for the oceanic holozooplankton assemblage. In addition, DNA barcodes allow recognition of new or undescribed species, reveal cryptic species within known taxa, and inform phylogeographic and population genetic studies of geographic variation. The growing database of “gold standard” DNA barcodes serves as a Rosetta Stone for marine zooplankton, providing the key for decoding species diversity by linking species names, morphology, and DNA sequence variation. In light of the pivotal position of zooplankton in ocean food webs, their usefulness as rapid responders to environmental change, and the increasing scarcity of taxonomists, the use of DNA barcodes is an important and useful approach for rapid analysis of species diversity and distribution in the pelagic community. Article in Journal/Newspaper Northwest Atlantic Nova Southeastern University: NSU Works |
institution |
Open Polar |
collection |
Nova Southeastern University: NSU Works |
op_collection_id |
ftnsoutheastern |
language |
unknown |
topic |
Zooplankton DNA barcode Sargasso Sea Species diversity Deep sea Marine Biology Oceanography and Atmospheric Sciences and Meteorology |
spellingShingle |
Zooplankton DNA barcode Sargasso Sea Species diversity Deep sea Marine Biology Oceanography and Atmospheric Sciences and Meteorology Bucklin, Ann Ortman, Brian D. Jennings, Robert M. Nigro, Lisa M. Sweetman, Christopher J. Copley, Nancy J. Sutton, Tracey Wiebe, Peter A “Rosetta Stone” for Metazoan Zooplankton: DNA Barcode Analysis of Species Diversity of the Sargasso Sea (Northwest Atlantic Ocean) |
topic_facet |
Zooplankton DNA barcode Sargasso Sea Species diversity Deep sea Marine Biology Oceanography and Atmospheric Sciences and Meteorology |
description |
Species diversity of the metazoan holozooplankton assemblage of the Sargasso Sea, Northwest Atlantic Ocean, was examined through coordinated morphological taxonomic identification of species and DNA sequencing of a ∼650 base-pair region of mitochondrial cytochrome oxidase I (mtCOI) as a DNA barcode (i.e., short sequence for species recognition and discrimination). Zooplankton collections were made from the surface to 5,000 meters during April, 2006 on the R/V R.H. Brown. Samples were examined by a ship-board team of morphological taxonomists; DNA barcoding was carried out in both ship-board and land-based DNA sequencing laboratories. DNA barcodes were determined for a total of 297 individuals of 175 holozooplankton species in four phyla, including: Cnidaria (Hydromedusae, 4 species; Siphonophora, 47); Arthropoda (Amphipoda, 10; Copepoda, 34; Decapoda, 9; Euphausiacea, 10; Mysidacea, 1; Ostracoda, 27); and Mollusca (Cephalopoda, 8; Heteropoda, 6; Pteropoda, 15); and Chaetognatha (4). Thirty species of fish (Teleostei) were also barcoded. For all seven zooplankton groups for which sufficient data were available, Kimura-2-Parameter genetic distances were significantly lower between individuals of the same species (mean=0.0114; S.D. 0.0117) than between individuals of different species within the same group (mean=0.3166; S.D. 0.0378). This difference, known as the barcode gap, ensures that mtCOI sequences are reliable characters for species identification for the oceanic holozooplankton assemblage. In addition, DNA barcodes allow recognition of new or undescribed species, reveal cryptic species within known taxa, and inform phylogeographic and population genetic studies of geographic variation. The growing database of “gold standard” DNA barcodes serves as a Rosetta Stone for marine zooplankton, providing the key for decoding species diversity by linking species names, morphology, and DNA sequence variation. In light of the pivotal position of zooplankton in ocean food webs, their usefulness as rapid responders to environmental change, and the increasing scarcity of taxonomists, the use of DNA barcodes is an important and useful approach for rapid analysis of species diversity and distribution in the pelagic community. |
format |
Article in Journal/Newspaper |
author |
Bucklin, Ann Ortman, Brian D. Jennings, Robert M. Nigro, Lisa M. Sweetman, Christopher J. Copley, Nancy J. Sutton, Tracey Wiebe, Peter |
author_facet |
Bucklin, Ann Ortman, Brian D. Jennings, Robert M. Nigro, Lisa M. Sweetman, Christopher J. Copley, Nancy J. Sutton, Tracey Wiebe, Peter |
author_sort |
Bucklin, Ann |
title |
A “Rosetta Stone” for Metazoan Zooplankton: DNA Barcode Analysis of Species Diversity of the Sargasso Sea (Northwest Atlantic Ocean) |
title_short |
A “Rosetta Stone” for Metazoan Zooplankton: DNA Barcode Analysis of Species Diversity of the Sargasso Sea (Northwest Atlantic Ocean) |
title_full |
A “Rosetta Stone” for Metazoan Zooplankton: DNA Barcode Analysis of Species Diversity of the Sargasso Sea (Northwest Atlantic Ocean) |
title_fullStr |
A “Rosetta Stone” for Metazoan Zooplankton: DNA Barcode Analysis of Species Diversity of the Sargasso Sea (Northwest Atlantic Ocean) |
title_full_unstemmed |
A “Rosetta Stone” for Metazoan Zooplankton: DNA Barcode Analysis of Species Diversity of the Sargasso Sea (Northwest Atlantic Ocean) |
title_sort |
“rosetta stone” for metazoan zooplankton: dna barcode analysis of species diversity of the sargasso sea (northwest atlantic ocean) |
publisher |
NSUWorks |
publishDate |
2010 |
url |
https://nsuworks.nova.edu/occ_facarticles/517 |
genre |
Northwest Atlantic |
genre_facet |
Northwest Atlantic |
op_source |
Marine & Environmental Sciences Faculty Articles |
op_relation |
https://nsuworks.nova.edu/occ_facarticles/517 |
_version_ |
1766148754648334336 |