Seasonal habitat drives intestinal microbiome composition in anadromous Arctic char (Salvelinus alpinus)
Intestinal microbial communities from 362 anadromous Arctic char (Salvelinus alpinus) from the high Arctic Kitikmeot region, Nunavut, Canada, were characterized using high-throughput 16S rRNA gene sequencing. The resulting bacterial communities were compared across four seasonal habitats that corres...
Published in: | Environmental Microbiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Society for Applied Microbiology and Wiley
2020
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Online Access: | https://doi.org/10.1111/1462-2920.15049 https://nrc-publications.canada.ca/eng/view/ft/?id=e1dec65f-f901-4ea3-b2d7-0ff5768e9134 https://nrc-publications.canada.ca/eng/view/object/?id=e1dec65f-f901-4ea3-b2d7-0ff5768e9134 https://nrc-publications.canada.ca/fra/voir/objet/?id=e1dec65f-f901-4ea3-b2d7-0ff5768e9134 |
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:e1dec65f-f901-4ea3-b2d7-0ff5768e9134 2023-05-15T14:39:37+02:00 Seasonal habitat drives intestinal microbiome composition in anadromous Arctic char (Salvelinus alpinus) Element, Geraint Engel, Katja Neufeld, Josh D. Casselman, John M. van Coeverden de Groot, Peter Greer, Charles W. Walker, Virginia K. 2020-06-04 text https://doi.org/10.1111/1462-2920.15049 https://nrc-publications.canada.ca/eng/view/ft/?id=e1dec65f-f901-4ea3-b2d7-0ff5768e9134 https://nrc-publications.canada.ca/eng/view/object/?id=e1dec65f-f901-4ea3-b2d7-0ff5768e9134 https://nrc-publications.canada.ca/fra/voir/objet/?id=e1dec65f-f901-4ea3-b2d7-0ff5768e9134 eng eng Society for Applied Microbiology and Wiley issn:1462-2912 issn:1462-2920 Environmental Microbiology, Publication date: 2020-06-04 doi:10.1111/1462-2920.15049 article 2020 ftnrccanada https://doi.org/10.1111/1462-2920.15049 2021-10-16T23:00:19Z Intestinal microbial communities from 362 anadromous Arctic char (Salvelinus alpinus) from the high Arctic Kitikmeot region, Nunavut, Canada, were characterized using high-throughput 16S rRNA gene sequencing. The resulting bacterial communities were compared across four seasonal habitats that correspond to different stages of annual migration. Arctic char intestinal communities differed by sampling site, salinity and stages of freshwater residence. Although microbiota from fish sampled in brackish water were broadly consistent with taxa seen in other anadromous salmonids, they were enriched with putative psychrophiles, including the nonluminous gut symbiont Photobacterium iliopiscarium that was detected in >90% of intestinal samples from these waters. Microbiota from freshwater-associated fish were less consistent with results reported for other salmonids, and highly variable, possibly reflecting winter fasting behaviour of these char. We identified microbiota links to age for those fish sampled during the autumn upriver migration, but little impact of the intestinal content and water microbiota on the intestinal community. The strongest driver of intestinal community composition was seasonal habitat, and this finding combined with identification of psychrophiles suggested that water temperature and migratory behaviour are key to understanding the relationship between Arctic char and their symbionts. Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper Arctic Kitikmeot Nunavut Salvelinus alpinus National Research Council Canada: NRC Publications Archive Arctic Nunavut Canada Environmental Microbiology 22 8 3112 3125 |
institution |
Open Polar |
collection |
National Research Council Canada: NRC Publications Archive |
op_collection_id |
ftnrccanada |
language |
English |
description |
Intestinal microbial communities from 362 anadromous Arctic char (Salvelinus alpinus) from the high Arctic Kitikmeot region, Nunavut, Canada, were characterized using high-throughput 16S rRNA gene sequencing. The resulting bacterial communities were compared across four seasonal habitats that correspond to different stages of annual migration. Arctic char intestinal communities differed by sampling site, salinity and stages of freshwater residence. Although microbiota from fish sampled in brackish water were broadly consistent with taxa seen in other anadromous salmonids, they were enriched with putative psychrophiles, including the nonluminous gut symbiont Photobacterium iliopiscarium that was detected in >90% of intestinal samples from these waters. Microbiota from freshwater-associated fish were less consistent with results reported for other salmonids, and highly variable, possibly reflecting winter fasting behaviour of these char. We identified microbiota links to age for those fish sampled during the autumn upriver migration, but little impact of the intestinal content and water microbiota on the intestinal community. The strongest driver of intestinal community composition was seasonal habitat, and this finding combined with identification of psychrophiles suggested that water temperature and migratory behaviour are key to understanding the relationship between Arctic char and their symbionts. Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Element, Geraint Engel, Katja Neufeld, Josh D. Casselman, John M. van Coeverden de Groot, Peter Greer, Charles W. Walker, Virginia K. |
spellingShingle |
Element, Geraint Engel, Katja Neufeld, Josh D. Casselman, John M. van Coeverden de Groot, Peter Greer, Charles W. Walker, Virginia K. Seasonal habitat drives intestinal microbiome composition in anadromous Arctic char (Salvelinus alpinus) |
author_facet |
Element, Geraint Engel, Katja Neufeld, Josh D. Casselman, John M. van Coeverden de Groot, Peter Greer, Charles W. Walker, Virginia K. |
author_sort |
Element, Geraint |
title |
Seasonal habitat drives intestinal microbiome composition in anadromous Arctic char (Salvelinus alpinus) |
title_short |
Seasonal habitat drives intestinal microbiome composition in anadromous Arctic char (Salvelinus alpinus) |
title_full |
Seasonal habitat drives intestinal microbiome composition in anadromous Arctic char (Salvelinus alpinus) |
title_fullStr |
Seasonal habitat drives intestinal microbiome composition in anadromous Arctic char (Salvelinus alpinus) |
title_full_unstemmed |
Seasonal habitat drives intestinal microbiome composition in anadromous Arctic char (Salvelinus alpinus) |
title_sort |
seasonal habitat drives intestinal microbiome composition in anadromous arctic char (salvelinus alpinus) |
publisher |
Society for Applied Microbiology and Wiley |
publishDate |
2020 |
url |
https://doi.org/10.1111/1462-2920.15049 https://nrc-publications.canada.ca/eng/view/ft/?id=e1dec65f-f901-4ea3-b2d7-0ff5768e9134 https://nrc-publications.canada.ca/eng/view/object/?id=e1dec65f-f901-4ea3-b2d7-0ff5768e9134 https://nrc-publications.canada.ca/fra/voir/objet/?id=e1dec65f-f901-4ea3-b2d7-0ff5768e9134 |
geographic |
Arctic Nunavut Canada |
geographic_facet |
Arctic Nunavut Canada |
genre |
Arctic Kitikmeot Nunavut Salvelinus alpinus |
genre_facet |
Arctic Kitikmeot Nunavut Salvelinus alpinus |
op_relation |
issn:1462-2912 issn:1462-2920 Environmental Microbiology, Publication date: 2020-06-04 doi:10.1111/1462-2920.15049 |
op_doi |
https://doi.org/10.1111/1462-2920.15049 |
container_title |
Environmental Microbiology |
container_volume |
22 |
container_issue |
8 |
container_start_page |
3112 |
op_container_end_page |
3125 |
_version_ |
1766311583292588032 |