Thiomicrorhabdus streamers and sulfur cycling in perennial hypersaline cold springs in the Canadian high Arctic
The Gypsum Hill (GH) springs on Axel Heiberg Island in the Canadian high Arctic are host to chemolithoautotrophic, sulfur-oxidizing streamers that flourish in the high Arctic winter in water temperatures from −1.3 to 7°C with ~8% salinity in a high Arctic winter environment with air temperatures com...
Published in: | Environmental Microbiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Society for Applied Microbiology
2020
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Online Access: | https://doi.org/10.1111/1462-2920.14916 https://nrc-publications.canada.ca/eng/view/object/?id=7947636a-9755-4485-8501-a85642cf1d1b https://nrc-publications.canada.ca/fra/voir/objet/?id=7947636a-9755-4485-8501-a85642cf1d1b |
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:7947636a-9755-4485-8501-a85642cf1d1b 2023-05-15T14:48:15+02:00 Thiomicrorhabdus streamers and sulfur cycling in perennial hypersaline cold springs in the Canadian high Arctic Magnuson, Elisse Mykytczuk, Nadia C.S. Pellerin, Andre Goordial, Jacqueline Twine, Susan M. Wing, Boswell Foote, Simon J. Fulton, Kelly Whyte, Lyle G. 2020-01-13 text https://doi.org/10.1111/1462-2920.14916 https://nrc-publications.canada.ca/eng/view/object/?id=7947636a-9755-4485-8501-a85642cf1d1b https://nrc-publications.canada.ca/fra/voir/objet/?id=7947636a-9755-4485-8501-a85642cf1d1b eng eng Society for Applied Microbiology issn:1462-2912 issn:1462-2920 Environmental Microbiology, Publication date: 2020-01-13 doi:10.1111/1462-2920.14916 article 2020 ftnrccanada https://doi.org/10.1111/1462-2920.14916 2021-09-25T23:00:12Z The Gypsum Hill (GH) springs on Axel Heiberg Island in the Canadian high Arctic are host to chemolithoautotrophic, sulfur-oxidizing streamers that flourish in the high Arctic winter in water temperatures from −1.3 to 7°C with ~8% salinity in a high Arctic winter environment with air temperatures commonly less than −40°C and an average annual air temperature of −15°C. Metagenome sequencing and binning of streamer samples produced a 96% complete Thiomicrorhabdus sp. metagenome-assembled genome representing a possible new species or subspecies. This is the most cold- and salt-extreme source environment for a Thiomicrorhabdus genome yet described. Metaproteomic and metatranscriptomic analysis attributed nearly all gene expression in the streamers to the Thiomicrorhabdus sp. and suggested that it is active in CO₂ fixation and oxidation of sulfide to elemental sulfur. In situ geochemical and isotopic analyses of the fractionation of multiple sulfur isotopes determined the biogeochemical transformation of sulfur from its source in Carboniferous evaporites to biotic processes occurring in the sediment and streamers. These complementary molecular tools provided a functional link between the geochemical substrates and the collective traits and activity that define the microbial community's interactions within a unique polar saline habitat where Thiomicrorhabdus-dominated streamers form and flourish. Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper Arctic Axel Heiberg Island National Research Council Canada: NRC Publications Archive Arctic Axel Heiberg Island ENVELOPE(-91.001,-91.001,79.752,79.752) Gypsum Hill ENVELOPE(-90.751,-90.751,79.402,79.402) Heiberg ENVELOPE(13.964,13.964,66.424,66.424) Environmental Microbiology 23 7 3384 3400 |
institution |
Open Polar |
collection |
National Research Council Canada: NRC Publications Archive |
op_collection_id |
ftnrccanada |
language |
English |
description |
The Gypsum Hill (GH) springs on Axel Heiberg Island in the Canadian high Arctic are host to chemolithoautotrophic, sulfur-oxidizing streamers that flourish in the high Arctic winter in water temperatures from −1.3 to 7°C with ~8% salinity in a high Arctic winter environment with air temperatures commonly less than −40°C and an average annual air temperature of −15°C. Metagenome sequencing and binning of streamer samples produced a 96% complete Thiomicrorhabdus sp. metagenome-assembled genome representing a possible new species or subspecies. This is the most cold- and salt-extreme source environment for a Thiomicrorhabdus genome yet described. Metaproteomic and metatranscriptomic analysis attributed nearly all gene expression in the streamers to the Thiomicrorhabdus sp. and suggested that it is active in CO₂ fixation and oxidation of sulfide to elemental sulfur. In situ geochemical and isotopic analyses of the fractionation of multiple sulfur isotopes determined the biogeochemical transformation of sulfur from its source in Carboniferous evaporites to biotic processes occurring in the sediment and streamers. These complementary molecular tools provided a functional link between the geochemical substrates and the collective traits and activity that define the microbial community's interactions within a unique polar saline habitat where Thiomicrorhabdus-dominated streamers form and flourish. Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Magnuson, Elisse Mykytczuk, Nadia C.S. Pellerin, Andre Goordial, Jacqueline Twine, Susan M. Wing, Boswell Foote, Simon J. Fulton, Kelly Whyte, Lyle G. |
spellingShingle |
Magnuson, Elisse Mykytczuk, Nadia C.S. Pellerin, Andre Goordial, Jacqueline Twine, Susan M. Wing, Boswell Foote, Simon J. Fulton, Kelly Whyte, Lyle G. Thiomicrorhabdus streamers and sulfur cycling in perennial hypersaline cold springs in the Canadian high Arctic |
author_facet |
Magnuson, Elisse Mykytczuk, Nadia C.S. Pellerin, Andre Goordial, Jacqueline Twine, Susan M. Wing, Boswell Foote, Simon J. Fulton, Kelly Whyte, Lyle G. |
author_sort |
Magnuson, Elisse |
title |
Thiomicrorhabdus streamers and sulfur cycling in perennial hypersaline cold springs in the Canadian high Arctic |
title_short |
Thiomicrorhabdus streamers and sulfur cycling in perennial hypersaline cold springs in the Canadian high Arctic |
title_full |
Thiomicrorhabdus streamers and sulfur cycling in perennial hypersaline cold springs in the Canadian high Arctic |
title_fullStr |
Thiomicrorhabdus streamers and sulfur cycling in perennial hypersaline cold springs in the Canadian high Arctic |
title_full_unstemmed |
Thiomicrorhabdus streamers and sulfur cycling in perennial hypersaline cold springs in the Canadian high Arctic |
title_sort |
thiomicrorhabdus streamers and sulfur cycling in perennial hypersaline cold springs in the canadian high arctic |
publisher |
Society for Applied Microbiology |
publishDate |
2020 |
url |
https://doi.org/10.1111/1462-2920.14916 https://nrc-publications.canada.ca/eng/view/object/?id=7947636a-9755-4485-8501-a85642cf1d1b https://nrc-publications.canada.ca/fra/voir/objet/?id=7947636a-9755-4485-8501-a85642cf1d1b |
long_lat |
ENVELOPE(-91.001,-91.001,79.752,79.752) ENVELOPE(-90.751,-90.751,79.402,79.402) ENVELOPE(13.964,13.964,66.424,66.424) |
geographic |
Arctic Axel Heiberg Island Gypsum Hill Heiberg |
geographic_facet |
Arctic Axel Heiberg Island Gypsum Hill Heiberg |
genre |
Arctic Axel Heiberg Island |
genre_facet |
Arctic Axel Heiberg Island |
op_relation |
issn:1462-2912 issn:1462-2920 Environmental Microbiology, Publication date: 2020-01-13 doi:10.1111/1462-2920.14916 |
op_doi |
https://doi.org/10.1111/1462-2920.14916 |
container_title |
Environmental Microbiology |
container_volume |
23 |
container_issue |
7 |
container_start_page |
3384 |
op_container_end_page |
3400 |
_version_ |
1766319339753963520 |