Characterization of microbial biodiversity in permafrost from the Canadian high Arctic using culture dependent and culture independent methods
A combination of culture-dependent and culture-independent methodologies (Bacteria and Archaea 16S rRNA gene clone library analyses) was used to determine the microbial diversity present within a geographically distinct high Arctic permafrost sample. Culturable Bacteria isolates, identified by 16S r...
Published in: | FEMS Microbiology Ecology |
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Blackwell Publishing Ltd.
2007
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Online Access: | https://doi.org/10.1111/j.1574-6941.2006.00247.x https://nrc-publications.canada.ca/eng/view/object/?id=76e7d35d-f5a9-498f-a5c6-ca17710f5a50 https://nrc-publications.canada.ca/fra/voir/objet/?id=76e7d35d-f5a9-498f-a5c6-ca17710f5a50 |
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:3538799 2023-05-15T14:57:53+02:00 Characterization of microbial biodiversity in permafrost from the Canadian high Arctic using culture dependent and culture independent methods Steven, Blaire Briggs, Geoffrey McKay, Chris P. Pollard, Wayne H. Greer, Charles W. Whyte, Lyle G. 2007 text https://doi.org/10.1111/j.1574-6941.2006.00247.x https://nrc-publications.canada.ca/eng/view/object/?id=76e7d35d-f5a9-498f-a5c6-ca17710f5a50 https://nrc-publications.canada.ca/fra/voir/objet/?id=76e7d35d-f5a9-498f-a5c6-ca17710f5a50 unknown Blackwell Publishing Ltd. issn:1574-6941 FEMS Microbiology Ecology, Volume: 59, Issue: 2, Publication date: 2007, Pages: 513–523 doi:10.1111/j.1574-6941.2006.00247.x Permafrost microbial diversity culture-independent culture-dependent bacteria Euryarchaeota article 2007 ftnrccanada https://doi.org/10.1111/j.1574-6941.2006.00247.x 2021-09-01T06:30:36Z A combination of culture-dependent and culture-independent methodologies (Bacteria and Archaea 16S rRNA gene clone library analyses) was used to determine the microbial diversity present within a geographically distinct high Arctic permafrost sample. Culturable Bacteria isolates, identified by 16S rRNA gene sequencing, belonged to the phyla Firmicutes, Actinobacteria and Proteobacteria with spore-forming Firmicutes being the most abundant; the majority of the isolates (19/23) were psychrotolerant, some (11/23) were halotolerant, and three isolates grew at -5�C. A Bacteria 16S rRNA gene library containing 101 clones was composed of 42 phylotypes related to diverse phylogenetic groups including the Actinobacteria, Proteobacteria, Firmicutes, Cytophaga � Flavobacteria � Bacteroides, Planctomyces and Gemmatimonadetes; the bacterial 16S rRNA gene phylotypes were dominated by Actinobacteria- and Proteobacteria-related sequences. An Archaea 16S rRNA gene clone library containing 56 clones was made up of 11 phylotypes and contained sequences related to both of the major Archaea domains (Euryarchaeota and Crenarchaeota); the majority of sequences in the Archaea library were related to halophilic Archaea. Characterization of the microbial diversity existing within permafrost environments is important as it will lead to a better understanding of how microorganisms function and survive in such extreme cryoenvironments. Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper Arctic permafrost National Research Council Canada: NRC Publications Archive Arctic FEMS Microbiology Ecology 59 2 513 523 |
institution |
Open Polar |
collection |
National Research Council Canada: NRC Publications Archive |
op_collection_id |
ftnrccanada |
language |
unknown |
topic |
Permafrost microbial diversity culture-independent culture-dependent bacteria Euryarchaeota |
spellingShingle |
Permafrost microbial diversity culture-independent culture-dependent bacteria Euryarchaeota Steven, Blaire Briggs, Geoffrey McKay, Chris P. Pollard, Wayne H. Greer, Charles W. Whyte, Lyle G. Characterization of microbial biodiversity in permafrost from the Canadian high Arctic using culture dependent and culture independent methods |
topic_facet |
Permafrost microbial diversity culture-independent culture-dependent bacteria Euryarchaeota |
description |
A combination of culture-dependent and culture-independent methodologies (Bacteria and Archaea 16S rRNA gene clone library analyses) was used to determine the microbial diversity present within a geographically distinct high Arctic permafrost sample. Culturable Bacteria isolates, identified by 16S rRNA gene sequencing, belonged to the phyla Firmicutes, Actinobacteria and Proteobacteria with spore-forming Firmicutes being the most abundant; the majority of the isolates (19/23) were psychrotolerant, some (11/23) were halotolerant, and three isolates grew at -5�C. A Bacteria 16S rRNA gene library containing 101 clones was composed of 42 phylotypes related to diverse phylogenetic groups including the Actinobacteria, Proteobacteria, Firmicutes, Cytophaga � Flavobacteria � Bacteroides, Planctomyces and Gemmatimonadetes; the bacterial 16S rRNA gene phylotypes were dominated by Actinobacteria- and Proteobacteria-related sequences. An Archaea 16S rRNA gene clone library containing 56 clones was made up of 11 phylotypes and contained sequences related to both of the major Archaea domains (Euryarchaeota and Crenarchaeota); the majority of sequences in the Archaea library were related to halophilic Archaea. Characterization of the microbial diversity existing within permafrost environments is important as it will lead to a better understanding of how microorganisms function and survive in such extreme cryoenvironments. Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Steven, Blaire Briggs, Geoffrey McKay, Chris P. Pollard, Wayne H. Greer, Charles W. Whyte, Lyle G. |
author_facet |
Steven, Blaire Briggs, Geoffrey McKay, Chris P. Pollard, Wayne H. Greer, Charles W. Whyte, Lyle G. |
author_sort |
Steven, Blaire |
title |
Characterization of microbial biodiversity in permafrost from the Canadian high Arctic using culture dependent and culture independent methods |
title_short |
Characterization of microbial biodiversity in permafrost from the Canadian high Arctic using culture dependent and culture independent methods |
title_full |
Characterization of microbial biodiversity in permafrost from the Canadian high Arctic using culture dependent and culture independent methods |
title_fullStr |
Characterization of microbial biodiversity in permafrost from the Canadian high Arctic using culture dependent and culture independent methods |
title_full_unstemmed |
Characterization of microbial biodiversity in permafrost from the Canadian high Arctic using culture dependent and culture independent methods |
title_sort |
characterization of microbial biodiversity in permafrost from the canadian high arctic using culture dependent and culture independent methods |
publisher |
Blackwell Publishing Ltd. |
publishDate |
2007 |
url |
https://doi.org/10.1111/j.1574-6941.2006.00247.x https://nrc-publications.canada.ca/eng/view/object/?id=76e7d35d-f5a9-498f-a5c6-ca17710f5a50 https://nrc-publications.canada.ca/fra/voir/objet/?id=76e7d35d-f5a9-498f-a5c6-ca17710f5a50 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic permafrost |
genre_facet |
Arctic permafrost |
op_relation |
issn:1574-6941 FEMS Microbiology Ecology, Volume: 59, Issue: 2, Publication date: 2007, Pages: 513–523 doi:10.1111/j.1574-6941.2006.00247.x |
op_doi |
https://doi.org/10.1111/j.1574-6941.2006.00247.x |
container_title |
FEMS Microbiology Ecology |
container_volume |
59 |
container_issue |
2 |
container_start_page |
513 |
op_container_end_page |
523 |
_version_ |
1766329989675876352 |