Identification of genes differently expressed in Atlantic salmon (Salmo salar) in response to infection by Aermonas salmonicida using cDNA microarray technology
The response of Atlantic salmon, Salmo salar, to infection by the bacterial pathogen Aeromonas salmonicida (the causative agent of furunculosis), was investigated using a cohabitation model and a custom Atlantic salmon cDNA microarray consisting of over 4000 different amplicons. Pooled samples of ea...
| Published in: | Developmental & Comparative Immunology |
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| Main Authors: | , , , , , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Elsevier
2005
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| Subjects: | |
| Online Access: | https://doi.org/10.1016/j.dci.2004.08.004 https://nrc-publications.canada.ca/eng/view/object/?id=86a1a81b-f791-4c77-9f46-e195d827adaa https://nrc-publications.canada.ca/fra/voir/objet/?id=86a1a81b-f791-4c77-9f46-e195d827adaa |
| Summary: | The response of Atlantic salmon, Salmo salar, to infection by the bacterial pathogen Aeromonas salmonicida (the causative agent of furunculosis), was investigated using a cohabitation model and a custom Atlantic salmon cDNA microarray consisting of over 4000 different amplicons. Pooled samples of each of three immune-relevant tissues (spleen, head kidney and liver) were obtained from fish exposed to infected salmon for 13 days. Reverse transcription-PCR assays were used to verify the differential expression of 12 candidate genes uncovered by microarray analysis. Among the differentially expressed genes were several previously revealed by suppression subtractive hybridization and EST surveys and that are recognized to encode humoral components of the innate immune system. Other genes identified in this study were not previously associated with infection. In addition, a number of genes with no known homologs were uncovered. Determination of their specific roles during infection may lead to a better understanding of innate immunity. Peer reviewed: Yes NRC publication: Yes |
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