Differential gene expression in Atlantic salmon infected with Lepeophtheirus salmonis
Lepeophtheirus salmonis is an ectoparasitic copepod that can cause serious disease outbreaks in salmonids. Previous work has shown that Atlantic salmon Salmo salar show very little, if any, tissue response to infection, whereas less-susceptible host species exhibit strong inflammatory responses. The...
Published in: | Journal of Aquatic Animal Health |
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Taylor & Francis Group
2011
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Online Access: | https://doi.org/10.1577/H05-043.1 https://nrc-publications.canada.ca/eng/view/object/?id=be9d7565-a85a-4b46-ba2a-89e0ed3e2ff2 https://nrc-publications.canada.ca/fra/voir/objet/?id=be9d7565-a85a-4b46-ba2a-89e0ed3e2ff2 |
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:3538386 2023-05-15T15:30:15+02:00 Differential gene expression in Atlantic salmon infected with Lepeophtheirus salmonis Fast, Mark D. Ross, Neil W. Johnson, Stewart C. 2011-01-09 text https://doi.org/10.1577/H05-043.1 https://nrc-publications.canada.ca/eng/view/object/?id=be9d7565-a85a-4b46-ba2a-89e0ed3e2ff2 https://nrc-publications.canada.ca/fra/voir/objet/?id=be9d7565-a85a-4b46-ba2a-89e0ed3e2ff2 eng eng Taylor & Francis Group issn:0899-7659 Journal of Aquatic Animal Health, Volume: 18, Issue: 2, Publication date: 2011-01-09, Pages: 116–127 doi:10.1577/H05-043.1 anadromous species biological stress ectoparasites fish diseases gene expression hormones leukocytes nucleotide sequence Lepeophtheirus salmonis Salmo salar article 2011 ftnrccanada https://doi.org/10.1577/H05-043.1 2021-09-01T06:26:10Z Lepeophtheirus salmonis is an ectoparasitic copepod that can cause serious disease outbreaks in salmonids. Previous work has shown that Atlantic salmon Salmo salar show very little, if any, tissue response to infection, whereas less-susceptible host species exhibit strong inflammatory responses. The objective of this study was to examine whether a low-level L. salmonis infection in the absence of a stress response would have an effect on expression of Atlantic salmon immune-related genes over time. The effects of low-level infection (approximately 8-11 lice/fish) on kidney tissue and head kidney leukocyte immune-related gene expression were studied at two postinfection time points. At this level of infection, there was no evidence of a cortisol stress response. By use of real-time polymerase chain reaction, constitutive expression of the major histocompatibility (MH) class I gene was shown to be 2-10-fold lower in infected Atlantic salmon head kidneys by 21 d postinfection (dpi) than in head kidneys of uninfected fish held under the same conditions. Conversely, by 14 and 21 dpi, constitutive MH class II expression was significantly increased (>10-fold) in infected fish. Constitutive expression of interleukin (IL) 1 beta also increased threefold in head kidneys of infected fish by 21 dpi; however, no differences were observed in cyclooxygenase (COX)-2 expression over the course of the infection. Adherent head kidney leukocytes from control Atlantic salmon showed an increase in MH class I and COX-2 expression after 3 h of lipopolysaccharide (LPS) stimulation for all three time points collected. The use of LPS stimulation on similar leukocytes from infected fish did not further increase levels of MH class I and COX-2 expression. This is the first report on host gene expression during an ectoparasitic copepod infection. The implications of these expression changes will be discussed with respect to the host-parasite relationship. Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper Atlantic salmon Salmo salar National Research Council Canada: NRC Publications Archive Journal of Aquatic Animal Health 18 2 116 127 |
institution |
Open Polar |
collection |
National Research Council Canada: NRC Publications Archive |
op_collection_id |
ftnrccanada |
language |
English |
topic |
anadromous species biological stress ectoparasites fish diseases gene expression hormones leukocytes nucleotide sequence Lepeophtheirus salmonis Salmo salar |
spellingShingle |
anadromous species biological stress ectoparasites fish diseases gene expression hormones leukocytes nucleotide sequence Lepeophtheirus salmonis Salmo salar Fast, Mark D. Ross, Neil W. Johnson, Stewart C. Differential gene expression in Atlantic salmon infected with Lepeophtheirus salmonis |
topic_facet |
anadromous species biological stress ectoparasites fish diseases gene expression hormones leukocytes nucleotide sequence Lepeophtheirus salmonis Salmo salar |
description |
Lepeophtheirus salmonis is an ectoparasitic copepod that can cause serious disease outbreaks in salmonids. Previous work has shown that Atlantic salmon Salmo salar show very little, if any, tissue response to infection, whereas less-susceptible host species exhibit strong inflammatory responses. The objective of this study was to examine whether a low-level L. salmonis infection in the absence of a stress response would have an effect on expression of Atlantic salmon immune-related genes over time. The effects of low-level infection (approximately 8-11 lice/fish) on kidney tissue and head kidney leukocyte immune-related gene expression were studied at two postinfection time points. At this level of infection, there was no evidence of a cortisol stress response. By use of real-time polymerase chain reaction, constitutive expression of the major histocompatibility (MH) class I gene was shown to be 2-10-fold lower in infected Atlantic salmon head kidneys by 21 d postinfection (dpi) than in head kidneys of uninfected fish held under the same conditions. Conversely, by 14 and 21 dpi, constitutive MH class II expression was significantly increased (>10-fold) in infected fish. Constitutive expression of interleukin (IL) 1 beta also increased threefold in head kidneys of infected fish by 21 dpi; however, no differences were observed in cyclooxygenase (COX)-2 expression over the course of the infection. Adherent head kidney leukocytes from control Atlantic salmon showed an increase in MH class I and COX-2 expression after 3 h of lipopolysaccharide (LPS) stimulation for all three time points collected. The use of LPS stimulation on similar leukocytes from infected fish did not further increase levels of MH class I and COX-2 expression. This is the first report on host gene expression during an ectoparasitic copepod infection. The implications of these expression changes will be discussed with respect to the host-parasite relationship. Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Fast, Mark D. Ross, Neil W. Johnson, Stewart C. |
author_facet |
Fast, Mark D. Ross, Neil W. Johnson, Stewart C. |
author_sort |
Fast, Mark D. |
title |
Differential gene expression in Atlantic salmon infected with Lepeophtheirus salmonis |
title_short |
Differential gene expression in Atlantic salmon infected with Lepeophtheirus salmonis |
title_full |
Differential gene expression in Atlantic salmon infected with Lepeophtheirus salmonis |
title_fullStr |
Differential gene expression in Atlantic salmon infected with Lepeophtheirus salmonis |
title_full_unstemmed |
Differential gene expression in Atlantic salmon infected with Lepeophtheirus salmonis |
title_sort |
differential gene expression in atlantic salmon infected with lepeophtheirus salmonis |
publisher |
Taylor & Francis Group |
publishDate |
2011 |
url |
https://doi.org/10.1577/H05-043.1 https://nrc-publications.canada.ca/eng/view/object/?id=be9d7565-a85a-4b46-ba2a-89e0ed3e2ff2 https://nrc-publications.canada.ca/fra/voir/objet/?id=be9d7565-a85a-4b46-ba2a-89e0ed3e2ff2 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
issn:0899-7659 Journal of Aquatic Animal Health, Volume: 18, Issue: 2, Publication date: 2011-01-09, Pages: 116–127 doi:10.1577/H05-043.1 |
op_doi |
https://doi.org/10.1577/H05-043.1 |
container_title |
Journal of Aquatic Animal Health |
container_volume |
18 |
container_issue |
2 |
container_start_page |
116 |
op_container_end_page |
127 |
_version_ |
1766360698204454912 |