Immunological responses of turbot (Psetta maxima) to nodavirus infection or polyriboinosinic polyribocytidylic acid (pIC) stimulation, using expressed sequence tags (ESTs) analysis and cDNA microarrays
To investigate the immunological responses of turbot to nodavirus infection or pIC stimulation, we constructed cDNA libraries from liver, kidney and gill tissues of nodavirus-infected fish and examined the differential gene expression within turbot kidney in response to nodavirus infection or pIC st...
Published in: | Fish & Shellfish Immunology |
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Online Access: | https://doi.org/10.1016/j.fsi.2008.03.010 https://nrc-publications.canada.ca/eng/view/object/?id=21771f41-af04-46a0-843c-044832ef2f5e https://nrc-publications.canada.ca/fra/voir/objet/?id=21771f41-af04-46a0-843c-044832ef2f5e |
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:3538382 2023-05-15T18:40:56+02:00 Immunological responses of turbot (Psetta maxima) to nodavirus infection or polyriboinosinic polyribocytidylic acid (pIC) stimulation, using expressed sequence tags (ESTs) analysis and cDNA microarrays Immunological responses of turbot (Psetta maxima) to nodavirus or poly I:C stimulation, using expressed sequence tags (ESTs) analysis and cDNA microarrays Park, Kyoung C. Osborne, Jane A. Montes, Ariana Dios, Sonia Nerland, Audund H. Novoa, Beatriz Figueras, Antonio Brown, Laura L. Johnson, Stewart C. 2008-03-26 text https://doi.org/10.1016/j.fsi.2008.03.010 https://nrc-publications.canada.ca/eng/view/object/?id=21771f41-af04-46a0-843c-044832ef2f5e https://nrc-publications.canada.ca/fra/voir/objet/?id=21771f41-af04-46a0-843c-044832ef2f5e eng eng Elsevier Fish and Shellfish Immunology, Volume: 26, Issue: 1, Publication date: 2008-03-26, Pages: 91–108 doi:10.1016/j.fsi.2008.03.010 Turbot Nodavirus pIC infection immune EST microarrays article 2008 ftnrccanada https://doi.org/10.1016/j.fsi.2008.03.010 2021-09-01T06:22:26Z To investigate the immunological responses of turbot to nodavirus infection or pIC stimulation, we constructed cDNA libraries from liver, kidney and gill tissues of nodavirus-infected fish and examined the differential gene expression within turbot kidney in response to nodavirus infection or pIC stimulation using a turbot cDNA microarray. Turbot were experimentally infected with nodavirus and samples of each tissue were collected at selected time points post-infection. Using equal amount of total RNA at each sampling time, we made three tissue-specific cDNA libraries. After sequencing 3230 clones we obtained 3173 (98.2%) high quality sequences from our liver, kidney and gill libraries. Of these 2568 (80.9%) were identified as known genes and 605 (19.1%) as unknown genes. A total of 768 unique genes were identified. The two largest groups resulting from the classification of ESTs according to function were the cell/organism defense genes (71 uni-genes) and apoptosis-related process (23 uni-genes). Using these clones, a 1920 element cDNA microarray was constructed and used to investigate the differential gene expression within turbot in response to experimental nodavirus infection or pIC stimulation. Kidney tissue was collected at selected times post-infection (HPI) or stimulation (HPS), and total RNA was isolated for microarray analysis. Of the 1920 genes studied on the microarray, we identified a total of 121 differentially expressed genes in the kidney: 94 genes from nodavirus-infected animals and 79 genes from those stimulated with pIC. Within the nodavirus-infected fish we observed the highest number of differentially expressed genes at 24 HPI. Our results indicate that certain genes in turbot have important roles in immune responses to nodavirus infection and dsRNA stimulation. Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper Turbot National Research Council Canada: NRC Publications Archive Fish & Shellfish Immunology 26 1 91 108 |
institution |
Open Polar |
collection |
National Research Council Canada: NRC Publications Archive |
op_collection_id |
ftnrccanada |
language |
English |
topic |
Turbot Nodavirus pIC infection immune EST microarrays |
spellingShingle |
Turbot Nodavirus pIC infection immune EST microarrays Park, Kyoung C. Osborne, Jane A. Montes, Ariana Dios, Sonia Nerland, Audund H. Novoa, Beatriz Figueras, Antonio Brown, Laura L. Johnson, Stewart C. Immunological responses of turbot (Psetta maxima) to nodavirus infection or polyriboinosinic polyribocytidylic acid (pIC) stimulation, using expressed sequence tags (ESTs) analysis and cDNA microarrays |
topic_facet |
Turbot Nodavirus pIC infection immune EST microarrays |
description |
To investigate the immunological responses of turbot to nodavirus infection or pIC stimulation, we constructed cDNA libraries from liver, kidney and gill tissues of nodavirus-infected fish and examined the differential gene expression within turbot kidney in response to nodavirus infection or pIC stimulation using a turbot cDNA microarray. Turbot were experimentally infected with nodavirus and samples of each tissue were collected at selected time points post-infection. Using equal amount of total RNA at each sampling time, we made three tissue-specific cDNA libraries. After sequencing 3230 clones we obtained 3173 (98.2%) high quality sequences from our liver, kidney and gill libraries. Of these 2568 (80.9%) were identified as known genes and 605 (19.1%) as unknown genes. A total of 768 unique genes were identified. The two largest groups resulting from the classification of ESTs according to function were the cell/organism defense genes (71 uni-genes) and apoptosis-related process (23 uni-genes). Using these clones, a 1920 element cDNA microarray was constructed and used to investigate the differential gene expression within turbot in response to experimental nodavirus infection or pIC stimulation. Kidney tissue was collected at selected times post-infection (HPI) or stimulation (HPS), and total RNA was isolated for microarray analysis. Of the 1920 genes studied on the microarray, we identified a total of 121 differentially expressed genes in the kidney: 94 genes from nodavirus-infected animals and 79 genes from those stimulated with pIC. Within the nodavirus-infected fish we observed the highest number of differentially expressed genes at 24 HPI. Our results indicate that certain genes in turbot have important roles in immune responses to nodavirus infection and dsRNA stimulation. Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Park, Kyoung C. Osborne, Jane A. Montes, Ariana Dios, Sonia Nerland, Audund H. Novoa, Beatriz Figueras, Antonio Brown, Laura L. Johnson, Stewart C. |
author_facet |
Park, Kyoung C. Osborne, Jane A. Montes, Ariana Dios, Sonia Nerland, Audund H. Novoa, Beatriz Figueras, Antonio Brown, Laura L. Johnson, Stewart C. |
author_sort |
Park, Kyoung C. |
title |
Immunological responses of turbot (Psetta maxima) to nodavirus infection or polyriboinosinic polyribocytidylic acid (pIC) stimulation, using expressed sequence tags (ESTs) analysis and cDNA microarrays |
title_short |
Immunological responses of turbot (Psetta maxima) to nodavirus infection or polyriboinosinic polyribocytidylic acid (pIC) stimulation, using expressed sequence tags (ESTs) analysis and cDNA microarrays |
title_full |
Immunological responses of turbot (Psetta maxima) to nodavirus infection or polyriboinosinic polyribocytidylic acid (pIC) stimulation, using expressed sequence tags (ESTs) analysis and cDNA microarrays |
title_fullStr |
Immunological responses of turbot (Psetta maxima) to nodavirus infection or polyriboinosinic polyribocytidylic acid (pIC) stimulation, using expressed sequence tags (ESTs) analysis and cDNA microarrays |
title_full_unstemmed |
Immunological responses of turbot (Psetta maxima) to nodavirus infection or polyriboinosinic polyribocytidylic acid (pIC) stimulation, using expressed sequence tags (ESTs) analysis and cDNA microarrays |
title_sort |
immunological responses of turbot (psetta maxima) to nodavirus infection or polyriboinosinic polyribocytidylic acid (pic) stimulation, using expressed sequence tags (ests) analysis and cdna microarrays |
publisher |
Elsevier |
publishDate |
2008 |
url |
https://doi.org/10.1016/j.fsi.2008.03.010 https://nrc-publications.canada.ca/eng/view/object/?id=21771f41-af04-46a0-843c-044832ef2f5e https://nrc-publications.canada.ca/fra/voir/objet/?id=21771f41-af04-46a0-843c-044832ef2f5e |
genre |
Turbot |
genre_facet |
Turbot |
op_relation |
Fish and Shellfish Immunology, Volume: 26, Issue: 1, Publication date: 2008-03-26, Pages: 91–108 doi:10.1016/j.fsi.2008.03.010 |
op_doi |
https://doi.org/10.1016/j.fsi.2008.03.010 |
container_title |
Fish & Shellfish Immunology |
container_volume |
26 |
container_issue |
1 |
container_start_page |
91 |
op_container_end_page |
108 |
_version_ |
1766230402598436864 |