Development of a method to assess binding of astaxanthin to Atlantic salmon Salmo salar L. muscle proteins
Several methods were examined to characterize the binding between astaxanthin and salmon muscle protein(s) in order to provide tools for evaluation of the role of muscle proteins on astaxanthin retention in Atlantic salmon Salmo salar L. flesh. The methods included gel filtration chromatography, dis...
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:3538359 2023-05-15T15:31:05+02:00 Development of a method to assess binding of astaxanthin to Atlantic salmon Salmo salar L. muscle proteins Saha, Madhury R. Ross, Neil W. Gill, Tom A. Olsen, Rolf E. Lall, Santosh P. 2005-03 text https://doi.org/10.1111/j.1365-2109.2004.01205.x https://nrc-publications.canada.ca/eng/view/object/?id=09c2b741-0ea1-4c19-a561-571ffc6e2602 https://nrc-publications.canada.ca/fra/voir/objet/?id=09c2b741-0ea1-4c19-a561-571ffc6e2602 eng eng Aquaculture Research, Volume: 36, Issue: 4, Publication date: 2005-03, Pages: 336–343 doi:10.1111/j.1365-2109.2004.01205.x Copyright © 2005, Blackwell Publishing Ltd. analytical techniques methodology Muscles chemical reactions colour chromatographic techniques filtration Fish culture actin biochemical analysis proteins aquaculture techniques article 2005 ftnrccanada https://doi.org/10.1111/j.1365-2109.2004.01205.x 2021-09-01T06:21:59Z Several methods were examined to characterize the binding between astaxanthin and salmon muscle protein(s) in order to provide tools for evaluation of the role of muscle proteins on astaxanthin retention in Atlantic salmon Salmo salar L. flesh. The methods included gel filtration chromatography, displacement of a hydrophobic probe and ultrafiltration. With gel filtration chromatography, aggregation of astaxanthin under the experimental conditions was a major problem for the separation of bound astaxanthin from free astaxanthin because the apparent molecular weight of aggregated astaxanthin or astaxanthin micelles was in the range of protein–astaxanthin complexes. Displacement of the fluorescent probe 8-anilino-1-naphthalenesulphonate (ANS) was not effective as astaxanthin quenched the fluorophore so that displacement could not be observed. An ultrafiltration method was developed using 200-mM sodium cholate for dispersion of astaxanthin aggregates. This allowed unbound astaxanthin to be separated from bound astaxanthin using a 30-kDa filter. After salmon muscle proteins were solubilized in different fractions by sequential extraction using low ionic strength solutions, the astaxanthin binding of different fractions was assessed using the ultrafiltration method. The significant difference (P<0.05) observed in the astaxanthin binding of the various fractions suggests an application of this assay to detect differences in affinity of proteins for astaxanthin. The results also suggest that proteins other than actomyosin or actin can bind astaxanthin in Atlantic salmon flesh. This method can be used for the identification of astaxanthin-binding proteins in salmon flesh and other tissues. Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper Atlantic salmon Salmo salar National Research Council Canada: NRC Publications Archive Aquaculture Research 36 4 336 343 |
institution |
Open Polar |
collection |
National Research Council Canada: NRC Publications Archive |
op_collection_id |
ftnrccanada |
language |
English |
topic |
analytical techniques methodology Muscles chemical reactions colour chromatographic techniques filtration Fish culture actin biochemical analysis proteins aquaculture techniques |
spellingShingle |
analytical techniques methodology Muscles chemical reactions colour chromatographic techniques filtration Fish culture actin biochemical analysis proteins aquaculture techniques Saha, Madhury R. Ross, Neil W. Gill, Tom A. Olsen, Rolf E. Lall, Santosh P. Development of a method to assess binding of astaxanthin to Atlantic salmon Salmo salar L. muscle proteins |
topic_facet |
analytical techniques methodology Muscles chemical reactions colour chromatographic techniques filtration Fish culture actin biochemical analysis proteins aquaculture techniques |
description |
Several methods were examined to characterize the binding between astaxanthin and salmon muscle protein(s) in order to provide tools for evaluation of the role of muscle proteins on astaxanthin retention in Atlantic salmon Salmo salar L. flesh. The methods included gel filtration chromatography, displacement of a hydrophobic probe and ultrafiltration. With gel filtration chromatography, aggregation of astaxanthin under the experimental conditions was a major problem for the separation of bound astaxanthin from free astaxanthin because the apparent molecular weight of aggregated astaxanthin or astaxanthin micelles was in the range of protein–astaxanthin complexes. Displacement of the fluorescent probe 8-anilino-1-naphthalenesulphonate (ANS) was not effective as astaxanthin quenched the fluorophore so that displacement could not be observed. An ultrafiltration method was developed using 200-mM sodium cholate for dispersion of astaxanthin aggregates. This allowed unbound astaxanthin to be separated from bound astaxanthin using a 30-kDa filter. After salmon muscle proteins were solubilized in different fractions by sequential extraction using low ionic strength solutions, the astaxanthin binding of different fractions was assessed using the ultrafiltration method. The significant difference (P<0.05) observed in the astaxanthin binding of the various fractions suggests an application of this assay to detect differences in affinity of proteins for astaxanthin. The results also suggest that proteins other than actomyosin or actin can bind astaxanthin in Atlantic salmon flesh. This method can be used for the identification of astaxanthin-binding proteins in salmon flesh and other tissues. Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Saha, Madhury R. Ross, Neil W. Gill, Tom A. Olsen, Rolf E. Lall, Santosh P. |
author_facet |
Saha, Madhury R. Ross, Neil W. Gill, Tom A. Olsen, Rolf E. Lall, Santosh P. |
author_sort |
Saha, Madhury R. |
title |
Development of a method to assess binding of astaxanthin to Atlantic salmon Salmo salar L. muscle proteins |
title_short |
Development of a method to assess binding of astaxanthin to Atlantic salmon Salmo salar L. muscle proteins |
title_full |
Development of a method to assess binding of astaxanthin to Atlantic salmon Salmo salar L. muscle proteins |
title_fullStr |
Development of a method to assess binding of astaxanthin to Atlantic salmon Salmo salar L. muscle proteins |
title_full_unstemmed |
Development of a method to assess binding of astaxanthin to Atlantic salmon Salmo salar L. muscle proteins |
title_sort |
development of a method to assess binding of astaxanthin to atlantic salmon salmo salar l. muscle proteins |
publishDate |
2005 |
url |
https://doi.org/10.1111/j.1365-2109.2004.01205.x https://nrc-publications.canada.ca/eng/view/object/?id=09c2b741-0ea1-4c19-a561-571ffc6e2602 https://nrc-publications.canada.ca/fra/voir/objet/?id=09c2b741-0ea1-4c19-a561-571ffc6e2602 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Aquaculture Research, Volume: 36, Issue: 4, Publication date: 2005-03, Pages: 336–343 doi:10.1111/j.1365-2109.2004.01205.x |
op_rights |
Copyright © 2005, Blackwell Publishing Ltd. |
op_doi |
https://doi.org/10.1111/j.1365-2109.2004.01205.x |
container_title |
Aquaculture Research |
container_volume |
36 |
container_issue |
4 |
container_start_page |
336 |
op_container_end_page |
343 |
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1766361574529826816 |