Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.)
A study was conducted to compare astaxanthin binding ability of solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.). Muscle proteins of juvenile Atlantic salmon, haddock and halibut were solubilize...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2006
|
Subjects: | |
Online Access: | https://doi.org/10.1016/j.cbpb.2006.04.011 https://nrc-publications.canada.ca/eng/view/object/?id=ef198444-b6e0-4483-9d50-6105ea75423b https://nrc-publications.canada.ca/fra/voir/objet/?id=ef198444-b6e0-4483-9d50-6105ea75423b |
id |
ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:3538239 |
---|---|
record_format |
openpolar |
spelling |
ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:3538239 2023-05-15T15:28:43+02:00 Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.) Saha, Madhury R. Ross, Neil W. Olsen, Rolf E. Lall, Santosh P. 2006-05-04 text https://doi.org/10.1016/j.cbpb.2006.04.011 https://nrc-publications.canada.ca/eng/view/object/?id=ef198444-b6e0-4483-9d50-6105ea75423b https://nrc-publications.canada.ca/fra/voir/objet/?id=ef198444-b6e0-4483-9d50-6105ea75423b eng eng Elsevier Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Volume: 144, Issue: 4, Publication date: 2006-05-04, Pages: 488–495 doi:10.1016/j.cbpb.2006.04.011 Crown Copyright© 2006 Published by Elsevier Inc. All rights reserved. astaxanthin-binding Atlantic halibut Atlantic salmon fluorescence intensity haddock muscle proteins article 2006 ftnrccanada https://doi.org/10.1016/j.cbpb.2006.04.011 2021-09-01T06:27:36Z A study was conducted to compare astaxanthin binding ability of solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.). Muscle proteins of juvenile Atlantic salmon, haddock and halibut were solubilized by sequential extraction of muscle tissue using low ionic strength solutions. Electrophoretic protein profiles of the six solubilized fractions from these species were similar. Each solubilized fraction from the three species was examined for its relative astaxanthin binding capacity. The amount of bound astaxanthin was significantly different (P < 0.05) among the six fractions of each species. Significant differences in astaxanthin binding were only found for fractions A and E among the species. The amount of bound astaxanthin in various fractions of each species showed a good correlation (R2 = 0.80–0.92) with the ANS (8-anilino-1-naphthalenesulfonate) fluorescence intensity of those fractions. The pattern and extent of astaxanthin binding to the muscle proteins of juvenile salmon, haddock and halibut is comparable to that reported previously for adult Atlantic salmon [Saha, M.R., Ross, N.W., Gill, T.A., Olsen, R.E., Lall, S.P., 2005. Development of a method to assess binding of astaxanthin to Atlantic salmon S. salar L. muscle proteins. Aquacult. Res. 36, 336–343.]. These combined observations suggest that the carotenoid binding capacity of the muscle proteins of salmon is not the limiting factor in the deposition of carotenoid in their flesh. Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper Atlantic salmon Salmo salar National Research Council Canada: NRC Publications Archive Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 144 4 488 495 |
institution |
Open Polar |
collection |
National Research Council Canada: NRC Publications Archive |
op_collection_id |
ftnrccanada |
language |
English |
topic |
astaxanthin-binding Atlantic halibut Atlantic salmon fluorescence intensity haddock muscle proteins |
spellingShingle |
astaxanthin-binding Atlantic halibut Atlantic salmon fluorescence intensity haddock muscle proteins Saha, Madhury R. Ross, Neil W. Olsen, Rolf E. Lall, Santosh P. Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.) |
topic_facet |
astaxanthin-binding Atlantic halibut Atlantic salmon fluorescence intensity haddock muscle proteins |
description |
A study was conducted to compare astaxanthin binding ability of solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.). Muscle proteins of juvenile Atlantic salmon, haddock and halibut were solubilized by sequential extraction of muscle tissue using low ionic strength solutions. Electrophoretic protein profiles of the six solubilized fractions from these species were similar. Each solubilized fraction from the three species was examined for its relative astaxanthin binding capacity. The amount of bound astaxanthin was significantly different (P < 0.05) among the six fractions of each species. Significant differences in astaxanthin binding were only found for fractions A and E among the species. The amount of bound astaxanthin in various fractions of each species showed a good correlation (R2 = 0.80–0.92) with the ANS (8-anilino-1-naphthalenesulfonate) fluorescence intensity of those fractions. The pattern and extent of astaxanthin binding to the muscle proteins of juvenile salmon, haddock and halibut is comparable to that reported previously for adult Atlantic salmon [Saha, M.R., Ross, N.W., Gill, T.A., Olsen, R.E., Lall, S.P., 2005. Development of a method to assess binding of astaxanthin to Atlantic salmon S. salar L. muscle proteins. Aquacult. Res. 36, 336–343.]. These combined observations suggest that the carotenoid binding capacity of the muscle proteins of salmon is not the limiting factor in the deposition of carotenoid in their flesh. Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Saha, Madhury R. Ross, Neil W. Olsen, Rolf E. Lall, Santosh P. |
author_facet |
Saha, Madhury R. Ross, Neil W. Olsen, Rolf E. Lall, Santosh P. |
author_sort |
Saha, Madhury R. |
title |
Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.) |
title_short |
Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.) |
title_full |
Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.) |
title_fullStr |
Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.) |
title_full_unstemmed |
Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.) |
title_sort |
astaxanthin binding to solubilized muscle proteins of atlantic salmon (salmo salar l.), haddock (melanogrammus aeglefinus l.) and atlantic halibut (hippoglossus hippoglossus l.) |
publisher |
Elsevier |
publishDate |
2006 |
url |
https://doi.org/10.1016/j.cbpb.2006.04.011 https://nrc-publications.canada.ca/eng/view/object/?id=ef198444-b6e0-4483-9d50-6105ea75423b https://nrc-publications.canada.ca/fra/voir/objet/?id=ef198444-b6e0-4483-9d50-6105ea75423b |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Volume: 144, Issue: 4, Publication date: 2006-05-04, Pages: 488–495 doi:10.1016/j.cbpb.2006.04.011 |
op_rights |
Crown Copyright© 2006 Published by Elsevier Inc. All rights reserved. |
op_doi |
https://doi.org/10.1016/j.cbpb.2006.04.011 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
144 |
container_issue |
4 |
container_start_page |
488 |
op_container_end_page |
495 |
_version_ |
1766359052117344256 |