The early response of Atlantic salmon (Salmo salar) macrophages exposed in vitro to Aeromonas salmonicida cultured in broth and in fish
Aeromonas salmonicida is a fish pathogen that causes furunculosis. Virulent strains of this bacterium are able to infect salmonid macrophages and survive within them, although mechanisms favouring intracellular survival are not completely understood. It is known that A. salmonicida cultured in vivo...
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:3538120 2023-05-15T15:30:31+02:00 The early response of Atlantic salmon (Salmo salar) macrophages exposed in vitro to Aeromonas salmonicida cultured in broth and in fish The early response of Atlantic salmon (Salmo salar L) macrophages exposed in vitro to Aeromonas salmonicida cultured in broth and in fish Ewart, K. Vanya Williams, Jason Richards, Robert C. Gallant, Jeffrey W. Melville, Krista Douglas, Susan E. 2007-08-20 text https://nrc-publications.canada.ca/eng/view/object/?id=f29fc1f2-3e48-4dfa-8001-12d683a20318 https://nrc-publications.canada.ca/fra/voir/objet/?id=f29fc1f2-3e48-4dfa-8001-12d683a20318 eng eng Elsevier issn:0145-305X Developmental & Comparative Immunology, Volume: 32, Issue: 4, Publication date: 2007-08-20, Pages: 380–390 inflammation acute phase response fish microarray qPCR article 2007 ftnrccanada 2021-09-01T06:30:36Z Aeromonas salmonicida is a fish pathogen that causes furunculosis. Virulent strains of this bacterium are able to infect salmonid macrophages and survive within them, although mechanisms favouring intracellular survival are not completely understood. It is known that A. salmonicida cultured in vivo in the peritoneal cavity of the host undergoes changes in gene expression and surface architecture compared with cultures grown in vitro in broth. Therefore, in this study, the early macrophage responses to A. salmonicida grown in vivo and in vitro were compared. Macrophage-enriched cell preparations from head kidney of Atlantic salmon (Salmo salar) were infected in vitro in 96-well microtitre dishes and changes in gene expression during the infection process were monitored using a custom Atlantic salmon cDNA microarray. A. salmonicida cultures grown in tryptic soy broth and in peritoneal implants were used to infect the macrophages. The macrophages were harvested at 0.5, 1.0 and 2.0 h after addition of the bacteria to the medium. Significant changes in gene expression were evident by microarray analysis at 2.0 h post-infection in macrophages infected with broth-grown and implant-grown bacteria; however, qPCR analysis revealed earlier up-regulation of JunB and TNF-α in macrophages exposed to the implant-grown bacteria. Up-regulation of those genes and others is consistent with the effects of extracellular products of aeromonad bacteria on macrophages and also suggests initiation of the innate immune response. Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper Atlantic salmon Salmo salar National Research Council Canada: NRC Publications Archive |
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Open Polar |
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National Research Council Canada: NRC Publications Archive |
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English |
topic |
inflammation acute phase response fish microarray qPCR |
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inflammation acute phase response fish microarray qPCR Ewart, K. Vanya Williams, Jason Richards, Robert C. Gallant, Jeffrey W. Melville, Krista Douglas, Susan E. The early response of Atlantic salmon (Salmo salar) macrophages exposed in vitro to Aeromonas salmonicida cultured in broth and in fish |
topic_facet |
inflammation acute phase response fish microarray qPCR |
description |
Aeromonas salmonicida is a fish pathogen that causes furunculosis. Virulent strains of this bacterium are able to infect salmonid macrophages and survive within them, although mechanisms favouring intracellular survival are not completely understood. It is known that A. salmonicida cultured in vivo in the peritoneal cavity of the host undergoes changes in gene expression and surface architecture compared with cultures grown in vitro in broth. Therefore, in this study, the early macrophage responses to A. salmonicida grown in vivo and in vitro were compared. Macrophage-enriched cell preparations from head kidney of Atlantic salmon (Salmo salar) were infected in vitro in 96-well microtitre dishes and changes in gene expression during the infection process were monitored using a custom Atlantic salmon cDNA microarray. A. salmonicida cultures grown in tryptic soy broth and in peritoneal implants were used to infect the macrophages. The macrophages were harvested at 0.5, 1.0 and 2.0 h after addition of the bacteria to the medium. Significant changes in gene expression were evident by microarray analysis at 2.0 h post-infection in macrophages infected with broth-grown and implant-grown bacteria; however, qPCR analysis revealed earlier up-regulation of JunB and TNF-α in macrophages exposed to the implant-grown bacteria. Up-regulation of those genes and others is consistent with the effects of extracellular products of aeromonad bacteria on macrophages and also suggests initiation of the innate immune response. Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Ewart, K. Vanya Williams, Jason Richards, Robert C. Gallant, Jeffrey W. Melville, Krista Douglas, Susan E. |
author_facet |
Ewart, K. Vanya Williams, Jason Richards, Robert C. Gallant, Jeffrey W. Melville, Krista Douglas, Susan E. |
author_sort |
Ewart, K. Vanya |
title |
The early response of Atlantic salmon (Salmo salar) macrophages exposed in vitro to Aeromonas salmonicida cultured in broth and in fish |
title_short |
The early response of Atlantic salmon (Salmo salar) macrophages exposed in vitro to Aeromonas salmonicida cultured in broth and in fish |
title_full |
The early response of Atlantic salmon (Salmo salar) macrophages exposed in vitro to Aeromonas salmonicida cultured in broth and in fish |
title_fullStr |
The early response of Atlantic salmon (Salmo salar) macrophages exposed in vitro to Aeromonas salmonicida cultured in broth and in fish |
title_full_unstemmed |
The early response of Atlantic salmon (Salmo salar) macrophages exposed in vitro to Aeromonas salmonicida cultured in broth and in fish |
title_sort |
early response of atlantic salmon (salmo salar) macrophages exposed in vitro to aeromonas salmonicida cultured in broth and in fish |
publisher |
Elsevier |
publishDate |
2007 |
url |
https://nrc-publications.canada.ca/eng/view/object/?id=f29fc1f2-3e48-4dfa-8001-12d683a20318 https://nrc-publications.canada.ca/fra/voir/objet/?id=f29fc1f2-3e48-4dfa-8001-12d683a20318 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
issn:0145-305X Developmental & Comparative Immunology, Volume: 32, Issue: 4, Publication date: 2007-08-20, Pages: 380–390 |
_version_ |
1766360976621305856 |