Molecular detection and characterization of nodavirus in several marine fish species from the northeastern Atlantic
Nodaviruses (NNV) are responsible for causing disease outbreaks mainly in hatchery-reared larvae and juveniles of a wide variety of fishes throughout the world. This disease has seriously limited the culture of marine fishes over the last decade. In the Atlantic provinces of Canada, disease caused b...
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:3538087 2023-05-15T15:26:51+02:00 Molecular detection and characterization of nodavirus in several marine fish species from the northeastern Atlantic Gagne, N. Johnson, S. C. Cook-Versloot, M. MacKinnon, A. M. Olivier, G. 2004-12-13 text https://doi.org/10.3354/dao062181 https://nrc-publications.canada.ca/eng/view/object/?id=0d7a08a4-4e4d-4e75-b6e1-0098212d0b51 https://nrc-publications.canada.ca/fra/voir/objet/?id=0d7a08a4-4e4d-4e75-b6e1-0098212d0b51 eng eng Inter-Research Diseases of Aquatic Organisms, Volume: 62, Issue: 3, Publication date: 2004-12-13, Pages: 181–189 doi:10.3354/dao062181 © Inter-Research 2004 · www.int-res.com. Resale or republication not permitted without written consent of the publisher nodaviruses RT-PCR phylogenetic analysis Atlantic cod Haddock article 2004 ftnrccanada https://doi.org/10.3354/dao062181 2021-09-18T23:00:19Z Nodaviruses (NNV) are responsible for causing disease outbreaks mainly in hatchery-reared larvae and juveniles of a wide variety of fishes throughout the world. This disease has seriously limited the culture of marine fishes over the last decade. In the Atlantic provinces of Canada, disease caused by a nodavirus was first reported in juvenile Atlantic cod being reared in Nova Scotia, in 1999. More recently, disease outbreaks caused by nodavirus have been identified in hatchery-reared Atlantic cod and haddock in Newfoundland and New Brunswick, respectively, and along the east coast of the USA. The presence of NNV in wild Atlantic cod adults and wild winter flounder has also been reported. Nodaviruses were isolated from cultured Atlantic cod and haddock, as well as from wild winter flounder from a variety of geographical localities, and their virus coat (capsid) protein genes were partially sequenced. An analysis of the data indicates that all of the nodaviruses isolated from eastern North America were closely related to one another, but that they were distinct from the European isolates already sequenced. Regardless of host species, isolates from close geographical localities were more similar than those from distant geographical areas. At the protein level, differences in coat protein sequences were seen only for strains isolated from Atlantic cod originating from Newfoundland. Our results suggest that NNV may have been present in the Atlantic off Canada and on the east coast of the USA for some time, and has evolved to form a monophyletic group, distinct from other isolates found in cold-water species. Non-lethal methods for detection of NNV are necessary to develop management strategies for this disease, and would be an asset to diagnosticians and producers. Based on the results of this study, new primers were designed and developed for an improved RT-PCR assay able to detect North Atlantic nodaviruses in ovarian fluids, eggs and other tissues. The application of this test to field samples is discussed. Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper atlantic cod Newfoundland North Atlantic National Research Council Canada: NRC Publications Archive Canada Diseases of Aquatic Organisms 62 181 189 |
institution |
Open Polar |
collection |
National Research Council Canada: NRC Publications Archive |
op_collection_id |
ftnrccanada |
language |
English |
topic |
nodaviruses RT-PCR phylogenetic analysis Atlantic cod Haddock |
spellingShingle |
nodaviruses RT-PCR phylogenetic analysis Atlantic cod Haddock Gagne, N. Johnson, S. C. Cook-Versloot, M. MacKinnon, A. M. Olivier, G. Molecular detection and characterization of nodavirus in several marine fish species from the northeastern Atlantic |
topic_facet |
nodaviruses RT-PCR phylogenetic analysis Atlantic cod Haddock |
description |
Nodaviruses (NNV) are responsible for causing disease outbreaks mainly in hatchery-reared larvae and juveniles of a wide variety of fishes throughout the world. This disease has seriously limited the culture of marine fishes over the last decade. In the Atlantic provinces of Canada, disease caused by a nodavirus was first reported in juvenile Atlantic cod being reared in Nova Scotia, in 1999. More recently, disease outbreaks caused by nodavirus have been identified in hatchery-reared Atlantic cod and haddock in Newfoundland and New Brunswick, respectively, and along the east coast of the USA. The presence of NNV in wild Atlantic cod adults and wild winter flounder has also been reported. Nodaviruses were isolated from cultured Atlantic cod and haddock, as well as from wild winter flounder from a variety of geographical localities, and their virus coat (capsid) protein genes were partially sequenced. An analysis of the data indicates that all of the nodaviruses isolated from eastern North America were closely related to one another, but that they were distinct from the European isolates already sequenced. Regardless of host species, isolates from close geographical localities were more similar than those from distant geographical areas. At the protein level, differences in coat protein sequences were seen only for strains isolated from Atlantic cod originating from Newfoundland. Our results suggest that NNV may have been present in the Atlantic off Canada and on the east coast of the USA for some time, and has evolved to form a monophyletic group, distinct from other isolates found in cold-water species. Non-lethal methods for detection of NNV are necessary to develop management strategies for this disease, and would be an asset to diagnosticians and producers. Based on the results of this study, new primers were designed and developed for an improved RT-PCR assay able to detect North Atlantic nodaviruses in ovarian fluids, eggs and other tissues. The application of this test to field samples is discussed. Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Gagne, N. Johnson, S. C. Cook-Versloot, M. MacKinnon, A. M. Olivier, G. |
author_facet |
Gagne, N. Johnson, S. C. Cook-Versloot, M. MacKinnon, A. M. Olivier, G. |
author_sort |
Gagne, N. |
title |
Molecular detection and characterization of nodavirus in several marine fish species from the northeastern Atlantic |
title_short |
Molecular detection and characterization of nodavirus in several marine fish species from the northeastern Atlantic |
title_full |
Molecular detection and characterization of nodavirus in several marine fish species from the northeastern Atlantic |
title_fullStr |
Molecular detection and characterization of nodavirus in several marine fish species from the northeastern Atlantic |
title_full_unstemmed |
Molecular detection and characterization of nodavirus in several marine fish species from the northeastern Atlantic |
title_sort |
molecular detection and characterization of nodavirus in several marine fish species from the northeastern atlantic |
publisher |
Inter-Research |
publishDate |
2004 |
url |
https://doi.org/10.3354/dao062181 https://nrc-publications.canada.ca/eng/view/object/?id=0d7a08a4-4e4d-4e75-b6e1-0098212d0b51 https://nrc-publications.canada.ca/fra/voir/objet/?id=0d7a08a4-4e4d-4e75-b6e1-0098212d0b51 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
atlantic cod Newfoundland North Atlantic |
genre_facet |
atlantic cod Newfoundland North Atlantic |
op_relation |
Diseases of Aquatic Organisms, Volume: 62, Issue: 3, Publication date: 2004-12-13, Pages: 181–189 doi:10.3354/dao062181 |
op_rights |
© Inter-Research 2004 · www.int-res.com. Resale or republication not permitted without written consent of the publisher |
op_doi |
https://doi.org/10.3354/dao062181 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
62 |
container_start_page |
181 |
op_container_end_page |
189 |
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1766357333110161408 |