Erythrocyte heat shock protein responses to chronic (in vivo) and acute (in vitro) temperature challenge in diploid and triploid salmonids
This research investigated how ploidy level (diploid versus triploid) affects the heat shock protein (HSP) response in erythrocytes under different thermal stress regimes, both in vivo and in vitro, in Atlantic salmon (Salmo salar) and brook charr (Salvelinus fontinalis) in order to address the ques...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1016/j.cbpa.2017.01.007 https://nrc-publications.canada.ca/eng/view/object/?id=8a75305c-5c8f-4634-b61e-1be798bb757e https://nrc-publications.canada.ca/fra/voir/objet/?id=8a75305c-5c8f-4634-b61e-1be798bb757e |
id |
ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:23003056 |
---|---|
record_format |
openpolar |
spelling |
ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:23003056 2023-05-15T15:31:44+02:00 Erythrocyte heat shock protein responses to chronic (in vivo) and acute (in vitro) temperature challenge in diploid and triploid salmonids Saranyan, Pillai V. Ross, Neil W. Benfey, Tillmann J. 2017-04 text https://doi.org/10.1016/j.cbpa.2017.01.007 https://nrc-publications.canada.ca/eng/view/object/?id=8a75305c-5c8f-4634-b61e-1be798bb757e https://nrc-publications.canada.ca/fra/voir/objet/?id=8a75305c-5c8f-4634-b61e-1be798bb757e eng eng Elsevier issn:1095-6433 Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, Volume: 206, Publication date: 2017-04, Pages: 95–104 doi:10.1016/j.cbpa.2017.01.007 triploidy HSP stress response proteostasis salmonid article 2017 ftnrccanada https://doi.org/10.1016/j.cbpa.2017.01.007 2021-09-01T06:17:06Z This research investigated how ploidy level (diploid versus triploid) affects the heat shock protein (HSP) response in erythrocytes under different thermal stress regimes, both in vivo and in vitro, in Atlantic salmon (Salmo salar) and brook charr (Salvelinus fontinalis) in order to address the question of why triploids typically have reduced thermal tolerance. A preliminary study confirmed that identical volumes of diploid and triploid erythrocytes (which equates to a smaller number of larger cells for triploids compared to diploids) did not differ in total protein synthesis rates. After chronic (100 d) acclimation of fish to 5, 15 and 25 °C, triploid erythrocytes had lower HSP70, HSP90, heat shock factor 1 (HSF1) and ubiquitin (free and total) levels than diploids in both species. Furthermore, Atlantic salmon erythrocytes showed significantly higher protein breakdown (based on conjugated ubiquitin levels) in triploids than diploids after acute heat stress in vitro, but no significant difference was detected between ploidies after acute cold stress. These results indicate that: 1) triploid erythrocytes synthesize more total protein per cell than diploids as a result of increased cell size; 2) triploids have sufficient total HSP levels for survival under low stress conditions; and 3) the lower basal titres of HSPs in triploids may be a handicap when combating acute stress. Taken together, this suggests that triploids are limited in their ability to withstand thermal stress because of a reduced ability to maintain proteostasis under stressful conditions. Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper Atlantic salmon Salmo salar National Research Council Canada: NRC Publications Archive Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 206 95 104 |
institution |
Open Polar |
collection |
National Research Council Canada: NRC Publications Archive |
op_collection_id |
ftnrccanada |
language |
English |
topic |
triploidy HSP stress response proteostasis salmonid |
spellingShingle |
triploidy HSP stress response proteostasis salmonid Saranyan, Pillai V. Ross, Neil W. Benfey, Tillmann J. Erythrocyte heat shock protein responses to chronic (in vivo) and acute (in vitro) temperature challenge in diploid and triploid salmonids |
topic_facet |
triploidy HSP stress response proteostasis salmonid |
description |
This research investigated how ploidy level (diploid versus triploid) affects the heat shock protein (HSP) response in erythrocytes under different thermal stress regimes, both in vivo and in vitro, in Atlantic salmon (Salmo salar) and brook charr (Salvelinus fontinalis) in order to address the question of why triploids typically have reduced thermal tolerance. A preliminary study confirmed that identical volumes of diploid and triploid erythrocytes (which equates to a smaller number of larger cells for triploids compared to diploids) did not differ in total protein synthesis rates. After chronic (100 d) acclimation of fish to 5, 15 and 25 °C, triploid erythrocytes had lower HSP70, HSP90, heat shock factor 1 (HSF1) and ubiquitin (free and total) levels than diploids in both species. Furthermore, Atlantic salmon erythrocytes showed significantly higher protein breakdown (based on conjugated ubiquitin levels) in triploids than diploids after acute heat stress in vitro, but no significant difference was detected between ploidies after acute cold stress. These results indicate that: 1) triploid erythrocytes synthesize more total protein per cell than diploids as a result of increased cell size; 2) triploids have sufficient total HSP levels for survival under low stress conditions; and 3) the lower basal titres of HSPs in triploids may be a handicap when combating acute stress. Taken together, this suggests that triploids are limited in their ability to withstand thermal stress because of a reduced ability to maintain proteostasis under stressful conditions. Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Saranyan, Pillai V. Ross, Neil W. Benfey, Tillmann J. |
author_facet |
Saranyan, Pillai V. Ross, Neil W. Benfey, Tillmann J. |
author_sort |
Saranyan, Pillai V. |
title |
Erythrocyte heat shock protein responses to chronic (in vivo) and acute (in vitro) temperature challenge in diploid and triploid salmonids |
title_short |
Erythrocyte heat shock protein responses to chronic (in vivo) and acute (in vitro) temperature challenge in diploid and triploid salmonids |
title_full |
Erythrocyte heat shock protein responses to chronic (in vivo) and acute (in vitro) temperature challenge in diploid and triploid salmonids |
title_fullStr |
Erythrocyte heat shock protein responses to chronic (in vivo) and acute (in vitro) temperature challenge in diploid and triploid salmonids |
title_full_unstemmed |
Erythrocyte heat shock protein responses to chronic (in vivo) and acute (in vitro) temperature challenge in diploid and triploid salmonids |
title_sort |
erythrocyte heat shock protein responses to chronic (in vivo) and acute (in vitro) temperature challenge in diploid and triploid salmonids |
publisher |
Elsevier |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.cbpa.2017.01.007 https://nrc-publications.canada.ca/eng/view/object/?id=8a75305c-5c8f-4634-b61e-1be798bb757e https://nrc-publications.canada.ca/fra/voir/objet/?id=8a75305c-5c8f-4634-b61e-1be798bb757e |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
issn:1095-6433 Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, Volume: 206, Publication date: 2017-04, Pages: 95–104 doi:10.1016/j.cbpa.2017.01.007 |
op_doi |
https://doi.org/10.1016/j.cbpa.2017.01.007 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
206 |
container_start_page |
95 |
op_container_end_page |
104 |
_version_ |
1766362260465254400 |