Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada)
The increasing accessibility to navigation and offshore oil exploration brings risks of hydrocarbon releases in Arctic waters. Bioremediation of hydrocarbons is a promising mitigation strategy but challenges remain, particularly due to low microbial metabolic rates in cold, ice-covered seas. Hydroca...
Published in: | FEMS Microbiology Ecology |
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Language: | English |
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Oxford University Press
2016
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Online Access: | https://doi.org/10.1093/femsec/fiw130 https://nrc-publications.canada.ca/eng/view/accepted/?id=7c0aa52f-edde-4536-bc0b-f3df34a692ee https://nrc-publications.canada.ca/eng/view/object/?id=7c0aa52f-edde-4536-bc0b-f3df34a692ee https://nrc-publications.canada.ca/fra/voir/objet/?id=7c0aa52f-edde-4536-bc0b-f3df34a692ee |
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:23000460 2024-09-15T18:26:26+00:00 Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada) Garneau, Marie-Ève Michel, Christine Meisterhans, Guillaume Fortin, Nathalie King, Thomas L. Greer, Charles W. Lee, Kenneth 2016-07-06 text https://doi.org/10.1093/femsec/fiw130 https://nrc-publications.canada.ca/eng/view/accepted/?id=7c0aa52f-edde-4536-bc0b-f3df34a692ee https://nrc-publications.canada.ca/eng/view/object/?id=7c0aa52f-edde-4536-bc0b-f3df34a692ee https://nrc-publications.canada.ca/fra/voir/objet/?id=7c0aa52f-edde-4536-bc0b-f3df34a692ee eng eng Oxford University Press Federation of European Microbiological Societies issn:0168-6496 issn:1574-6941 FEMS Microbiology Ecology, Volume: 92, Issue: 10, Publication date: 2016-07-06, Pages: 1–18 doi:10.1093/femsec/fiw130 arctic sea ice hydrocarbon biodegradation bioremediation 16S ribosomal RNA gene sequencing microbial community fingerprinting article 2016 ftnrccanada https://doi.org/10.1093/femsec/fiw130 2024-08-05T14:05:07Z The increasing accessibility to navigation and offshore oil exploration brings risks of hydrocarbon releases in Arctic waters. Bioremediation of hydrocarbons is a promising mitigation strategy but challenges remain, particularly due to low microbial metabolic rates in cold, ice-covered seas. Hydrocarbon degradation potential of ice-associated microbes collected from the Northwest Passage was investigated. Microcosm incubations were run for 15 days at -1.7°C with and without oil to determine the effects of hydrocarbon exposure on microbial abundance, diversity and activity, and to estimate component-specific hydrocarbon loss. Diversity was assessed with automated ribosomal intergenic spacer analysis and ion torrent 16S rRNA gene sequencing. Bacterial activity was measured by ³H-leucine uptake rates. After incubation, sub-ice and sea-ice communities degraded 94% and 48% of the initial hydrocarbons, respectively. Hydrocarbon exposure changed the composition of sea-ice and sub-ice communities; in sea-ice microcosms, Bacteroidetes (mainly Polaribacter) dominated whereas in sub-ice microcosms, Epsilonproteobacteria contribution increased, but that of Alphaproteobacteria and Bacteroidetes decreased. Sequencing data revealed a decline in diversity and increases in Colwellia and Moritella in oil-treated microcosms. Low concentration of dissolved organic matter (DOM) in sub-ice seawater may explain higher hydrocarbon degradation when compared to sea ice, where DOM was abundant and composed of labile exopolysaccharides. Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper Northwest passage Nunavut Sea ice National Research Council Canada: NRC Publications Archive FEMS Microbiology Ecology 92 10 fiw130 |
institution |
Open Polar |
collection |
National Research Council Canada: NRC Publications Archive |
op_collection_id |
ftnrccanada |
language |
English |
topic |
arctic sea ice hydrocarbon biodegradation bioremediation 16S ribosomal RNA gene sequencing microbial community fingerprinting |
spellingShingle |
arctic sea ice hydrocarbon biodegradation bioremediation 16S ribosomal RNA gene sequencing microbial community fingerprinting Garneau, Marie-Ève Michel, Christine Meisterhans, Guillaume Fortin, Nathalie King, Thomas L. Greer, Charles W. Lee, Kenneth Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada) |
topic_facet |
arctic sea ice hydrocarbon biodegradation bioremediation 16S ribosomal RNA gene sequencing microbial community fingerprinting |
description |
The increasing accessibility to navigation and offshore oil exploration brings risks of hydrocarbon releases in Arctic waters. Bioremediation of hydrocarbons is a promising mitigation strategy but challenges remain, particularly due to low microbial metabolic rates in cold, ice-covered seas. Hydrocarbon degradation potential of ice-associated microbes collected from the Northwest Passage was investigated. Microcosm incubations were run for 15 days at -1.7°C with and without oil to determine the effects of hydrocarbon exposure on microbial abundance, diversity and activity, and to estimate component-specific hydrocarbon loss. Diversity was assessed with automated ribosomal intergenic spacer analysis and ion torrent 16S rRNA gene sequencing. Bacterial activity was measured by ³H-leucine uptake rates. After incubation, sub-ice and sea-ice communities degraded 94% and 48% of the initial hydrocarbons, respectively. Hydrocarbon exposure changed the composition of sea-ice and sub-ice communities; in sea-ice microcosms, Bacteroidetes (mainly Polaribacter) dominated whereas in sub-ice microcosms, Epsilonproteobacteria contribution increased, but that of Alphaproteobacteria and Bacteroidetes decreased. Sequencing data revealed a decline in diversity and increases in Colwellia and Moritella in oil-treated microcosms. Low concentration of dissolved organic matter (DOM) in sub-ice seawater may explain higher hydrocarbon degradation when compared to sea ice, where DOM was abundant and composed of labile exopolysaccharides. Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Garneau, Marie-Ève Michel, Christine Meisterhans, Guillaume Fortin, Nathalie King, Thomas L. Greer, Charles W. Lee, Kenneth |
author_facet |
Garneau, Marie-Ève Michel, Christine Meisterhans, Guillaume Fortin, Nathalie King, Thomas L. Greer, Charles W. Lee, Kenneth |
author_sort |
Garneau, Marie-Ève |
title |
Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada) |
title_short |
Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada) |
title_full |
Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada) |
title_fullStr |
Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada) |
title_full_unstemmed |
Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada) |
title_sort |
hydrocarbon biodegradation by arctic sea-ice and sub-ice microbial communities during microcosm experiments, northwest passage (nunavut, canada) |
publisher |
Oxford University Press |
publishDate |
2016 |
url |
https://doi.org/10.1093/femsec/fiw130 https://nrc-publications.canada.ca/eng/view/accepted/?id=7c0aa52f-edde-4536-bc0b-f3df34a692ee https://nrc-publications.canada.ca/eng/view/object/?id=7c0aa52f-edde-4536-bc0b-f3df34a692ee https://nrc-publications.canada.ca/fra/voir/objet/?id=7c0aa52f-edde-4536-bc0b-f3df34a692ee |
genre |
Northwest passage Nunavut Sea ice |
genre_facet |
Northwest passage Nunavut Sea ice |
op_relation |
issn:0168-6496 issn:1574-6941 FEMS Microbiology Ecology, Volume: 92, Issue: 10, Publication date: 2016-07-06, Pages: 1–18 doi:10.1093/femsec/fiw130 |
op_doi |
https://doi.org/10.1093/femsec/fiw130 |
container_title |
FEMS Microbiology Ecology |
container_volume |
92 |
container_issue |
10 |
container_start_page |
fiw130 |
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1810466929001889792 |