Evidence of in situ microbial activity and sulphidogenesis in perennially sub-0 °C and hypersaline sediments of a high Arctic permafrost spring
The lost hammer (LH) spring perennially discharges subzero hypersaline reducing brines through thick layers of permafrost and is the only known terrestrial methane seep in frozen settings on Earth. The present study aimed to identify active microbial communities that populate the sediments of the sp...
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Springer International Publishing
2014
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Online Access: | https://doi.org/10.1007/s00792-014-0703-4 https://nrc-publications.canada.ca/eng/view/object/?id=e13f4e23-dd98-452b-9593-e194624e0d61 https://nrc-publications.canada.ca/fra/voir/objet/?id=e13f4e23-dd98-452b-9593-e194624e0d61 |
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:21275555 2023-05-15T15:08:50+02:00 Evidence of in situ microbial activity and sulphidogenesis in perennially sub-0 °C and hypersaline sediments of a high Arctic permafrost spring Lamarche-Gagnon, Guillaume Comery, Raven Greer, Charles W. Whyte, Lyle G. 2014-11-09 text https://doi.org/10.1007/s00792-014-0703-4 https://nrc-publications.canada.ca/eng/view/object/?id=e13f4e23-dd98-452b-9593-e194624e0d61 https://nrc-publications.canada.ca/fra/voir/objet/?id=e13f4e23-dd98-452b-9593-e194624e0d61 eng eng Springer International Publishing issn:1431-0651 Extremophiles, 10th International Conference on Extremophiles, Sept. 7-11, 2014, Saint Petersburg, Russia, Volume: 19, Issue: 1, Publication date: 2014-11-09 doi:10.1007/s00792-014-0703-4 article 2014 ftnrccanada https://doi.org/10.1007/s00792-014-0703-4 2021-09-01T06:28:02Z The lost hammer (LH) spring perennially discharges subzero hypersaline reducing brines through thick layers of permafrost and is the only known terrestrial methane seep in frozen settings on Earth. The present study aimed to identify active microbial communities that populate the sediments of the spring outlet, and verify whether such communities vary seasonally and spatially. Microcosm experiments revealed that the biological reduction of sulfur compounds (SR) with hydrogen (e.g., sulfate reduction) was potentially carried out under combined hypersaline and subzero conditions, down to −20 °C, the coldest temperature ever recorded for SR. Pyrosequencing analyses of both 16S rRNA (i.e., cDNA) and 16S rRNA genes (i.e., DNA) of sediments retrieved in late winter and summer indicated fairly stable bacterial and archaeal communities at the phylum level. Potentially active bacterial and archaeal communities were dominated by clades related to the T78 Chloroflexi group and Halobacteria species, respectively. The present study indicated that SR, hydrogenotrophy (possibly coupled to autotrophy), and short-chain alkane degradation (other than methane), most likely represent important, previously unaccounted for, metabolic processes carried out by LH microbial communities. Overall, the obtained findings provided additional evidence that the LH system hosts active communities of anaerobic, halophilic, and cryophilic microorganisms despite the extreme conditions in situ. Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper Arctic permafrost National Research Council Canada: NRC Publications Archive Arctic Extremophiles 19 1 1 15 |
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Open Polar |
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National Research Council Canada: NRC Publications Archive |
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ftnrccanada |
language |
English |
description |
The lost hammer (LH) spring perennially discharges subzero hypersaline reducing brines through thick layers of permafrost and is the only known terrestrial methane seep in frozen settings on Earth. The present study aimed to identify active microbial communities that populate the sediments of the spring outlet, and verify whether such communities vary seasonally and spatially. Microcosm experiments revealed that the biological reduction of sulfur compounds (SR) with hydrogen (e.g., sulfate reduction) was potentially carried out under combined hypersaline and subzero conditions, down to −20 °C, the coldest temperature ever recorded for SR. Pyrosequencing analyses of both 16S rRNA (i.e., cDNA) and 16S rRNA genes (i.e., DNA) of sediments retrieved in late winter and summer indicated fairly stable bacterial and archaeal communities at the phylum level. Potentially active bacterial and archaeal communities were dominated by clades related to the T78 Chloroflexi group and Halobacteria species, respectively. The present study indicated that SR, hydrogenotrophy (possibly coupled to autotrophy), and short-chain alkane degradation (other than methane), most likely represent important, previously unaccounted for, metabolic processes carried out by LH microbial communities. Overall, the obtained findings provided additional evidence that the LH system hosts active communities of anaerobic, halophilic, and cryophilic microorganisms despite the extreme conditions in situ. Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Lamarche-Gagnon, Guillaume Comery, Raven Greer, Charles W. Whyte, Lyle G. |
spellingShingle |
Lamarche-Gagnon, Guillaume Comery, Raven Greer, Charles W. Whyte, Lyle G. Evidence of in situ microbial activity and sulphidogenesis in perennially sub-0 °C and hypersaline sediments of a high Arctic permafrost spring |
author_facet |
Lamarche-Gagnon, Guillaume Comery, Raven Greer, Charles W. Whyte, Lyle G. |
author_sort |
Lamarche-Gagnon, Guillaume |
title |
Evidence of in situ microbial activity and sulphidogenesis in perennially sub-0 °C and hypersaline sediments of a high Arctic permafrost spring |
title_short |
Evidence of in situ microbial activity and sulphidogenesis in perennially sub-0 °C and hypersaline sediments of a high Arctic permafrost spring |
title_full |
Evidence of in situ microbial activity and sulphidogenesis in perennially sub-0 °C and hypersaline sediments of a high Arctic permafrost spring |
title_fullStr |
Evidence of in situ microbial activity and sulphidogenesis in perennially sub-0 °C and hypersaline sediments of a high Arctic permafrost spring |
title_full_unstemmed |
Evidence of in situ microbial activity and sulphidogenesis in perennially sub-0 °C and hypersaline sediments of a high Arctic permafrost spring |
title_sort |
evidence of in situ microbial activity and sulphidogenesis in perennially sub-0 °c and hypersaline sediments of a high arctic permafrost spring |
publisher |
Springer International Publishing |
publishDate |
2014 |
url |
https://doi.org/10.1007/s00792-014-0703-4 https://nrc-publications.canada.ca/eng/view/object/?id=e13f4e23-dd98-452b-9593-e194624e0d61 https://nrc-publications.canada.ca/fra/voir/objet/?id=e13f4e23-dd98-452b-9593-e194624e0d61 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic permafrost |
genre_facet |
Arctic permafrost |
op_relation |
issn:1431-0651 Extremophiles, 10th International Conference on Extremophiles, Sept. 7-11, 2014, Saint Petersburg, Russia, Volume: 19, Issue: 1, Publication date: 2014-11-09 doi:10.1007/s00792-014-0703-4 |
op_doi |
https://doi.org/10.1007/s00792-014-0703-4 |
container_title |
Extremophiles |
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19 |
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1 |
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1 |
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15 |
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1766340113631019008 |