Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish
Antimicrobial peptides play a crucial role as the first line of defense against invading pathogens. Several types of antimicrobial peptides have been isolated from fish, mostly of the cationic α-helical variety. Here, we present the cDNA sequences of five highly disulphide-bonded hepcidin-like pepti...
Published in: | Developmental & Comparative Immunology |
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Online Access: | https://doi.org/10.1016/S0145-305X(03)00036-3 https://nrc-publications.canada.ca/eng/view/object/?id=40f6bd97-3484-4e23-bd9c-6ff4df1e847a https://nrc-publications.canada.ca/fra/voir/objet/?id=40f6bd97-3484-4e23-bd9c-6ff4df1e847a |
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:21272066 2023-05-15T15:32:24+02:00 Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish Douglas, S.E. Gallant, J.W. Liebscher, R.S. Dacanay, A. Tsoi, S.C.M. 2003-06 text https://doi.org/10.1016/S0145-305X(03)00036-3 https://nrc-publications.canada.ca/eng/view/object/?id=40f6bd97-3484-4e23-bd9c-6ff4df1e847a https://nrc-publications.canada.ca/fra/voir/objet/?id=40f6bd97-3484-4e23-bd9c-6ff4df1e847a eng eng issn:0145-305X Developmental and Comparative Immunology, Volume: 27, Issue: 6-7, Publication date: 2003-06, Pages: 589–601 doi:10.1016/S0145-305X(03)00036-3 antimicrobial peptide gene expression hepcidin larvae Pseudopleuronectes americanus Salmo salar salmon winter flounder article 2003 ftnrccanada https://doi.org/10.1016/S0145-305X(03)00036-3 2021-09-01T06:20:54Z Antimicrobial peptides play a crucial role as the first line of defense against invading pathogens. Several types of antimicrobial peptides have been isolated from fish, mostly of the cationic α-helical variety. Here, we present the cDNA sequences of five highly disulphide-bonded hepcidin-like peptides from winter flounder, Pseudopleuronectes americanus (Walbaum) and two from Atlantic salmon, Salmo salar (L.). These hepcidin-like molecules consist of a 24 amino acid signal peptide and an acidic propiece of 38-40 amino acids in addition to the mature processed peptide of 19-27 amino acids. Exhaustive data mining of GenBank with these sequences revealed that similar peptides are encoded in the genomes of Japanese flounder, rainbow trout, hybrid striped bass and medaka, indicating that they are widespread among fish. Southern hybridization analysis suggests that closely related hepcidin-like genes are present in other flatfish species, and that they exist as a multigene family clustered on the winter flounder genome. Hepcidin variants are differentially expressed during bacterial challenge, during larval development of P. americanus and in different tissues of adult fish. Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper Atlantic salmon Salmo salar National Research Council Canada: NRC Publications Archive Developmental & Comparative Immunology 27 6-7 589 601 |
institution |
Open Polar |
collection |
National Research Council Canada: NRC Publications Archive |
op_collection_id |
ftnrccanada |
language |
English |
topic |
antimicrobial peptide gene expression hepcidin larvae Pseudopleuronectes americanus Salmo salar salmon winter flounder |
spellingShingle |
antimicrobial peptide gene expression hepcidin larvae Pseudopleuronectes americanus Salmo salar salmon winter flounder Douglas, S.E. Gallant, J.W. Liebscher, R.S. Dacanay, A. Tsoi, S.C.M. Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish |
topic_facet |
antimicrobial peptide gene expression hepcidin larvae Pseudopleuronectes americanus Salmo salar salmon winter flounder |
description |
Antimicrobial peptides play a crucial role as the first line of defense against invading pathogens. Several types of antimicrobial peptides have been isolated from fish, mostly of the cationic α-helical variety. Here, we present the cDNA sequences of five highly disulphide-bonded hepcidin-like peptides from winter flounder, Pseudopleuronectes americanus (Walbaum) and two from Atlantic salmon, Salmo salar (L.). These hepcidin-like molecules consist of a 24 amino acid signal peptide and an acidic propiece of 38-40 amino acids in addition to the mature processed peptide of 19-27 amino acids. Exhaustive data mining of GenBank with these sequences revealed that similar peptides are encoded in the genomes of Japanese flounder, rainbow trout, hybrid striped bass and medaka, indicating that they are widespread among fish. Southern hybridization analysis suggests that closely related hepcidin-like genes are present in other flatfish species, and that they exist as a multigene family clustered on the winter flounder genome. Hepcidin variants are differentially expressed during bacterial challenge, during larval development of P. americanus and in different tissues of adult fish. Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Douglas, S.E. Gallant, J.W. Liebscher, R.S. Dacanay, A. Tsoi, S.C.M. |
author_facet |
Douglas, S.E. Gallant, J.W. Liebscher, R.S. Dacanay, A. Tsoi, S.C.M. |
author_sort |
Douglas, S.E. |
title |
Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish |
title_short |
Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish |
title_full |
Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish |
title_fullStr |
Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish |
title_full_unstemmed |
Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish |
title_sort |
identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish |
publishDate |
2003 |
url |
https://doi.org/10.1016/S0145-305X(03)00036-3 https://nrc-publications.canada.ca/eng/view/object/?id=40f6bd97-3484-4e23-bd9c-6ff4df1e847a https://nrc-publications.canada.ca/fra/voir/objet/?id=40f6bd97-3484-4e23-bd9c-6ff4df1e847a |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
issn:0145-305X Developmental and Comparative Immunology, Volume: 27, Issue: 6-7, Publication date: 2003-06, Pages: 589–601 doi:10.1016/S0145-305X(03)00036-3 |
op_doi |
https://doi.org/10.1016/S0145-305X(03)00036-3 |
container_title |
Developmental & Comparative Immunology |
container_volume |
27 |
container_issue |
6-7 |
container_start_page |
589 |
op_container_end_page |
601 |
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