Bacterial community evidence for anaerobic degradation of petroleum hydrocarbons in cold climate groundwater
There is currently limited scientific data to assess whether groundwater bacterial communities in fractured rock environments can degrade petroleum hydrocarbon plumes in cold regions. The former Colomac Mine is located in the Canadian Shield in the Northwest Territories (mean annual air temperature...
Published in: | Cold Regions Science and Technology |
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2013
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Online Access: | https://doi.org/10.1016/j.coldregions.2012.10.013 https://nrc-publications.canada.ca/eng/view/object/?id=25d67768-c808-4c0f-88c2-94c5bbb4501c https://nrc-publications.canada.ca/fra/voir/objet/?id=25d67768-c808-4c0f-88c2-94c5bbb4501c |
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:21270395 2023-05-15T17:46:39+02:00 Bacterial community evidence for anaerobic degradation of petroleum hydrocarbons in cold climate groundwater Yeung, C. William Van Stempvoort, Dale R. Spoelstra, John Bickerton, Greg Voralek, John Greer, Charles W. 2013-11-14 text https://doi.org/10.1016/j.coldregions.2012.10.013 https://nrc-publications.canada.ca/eng/view/object/?id=25d67768-c808-4c0f-88c2-94c5bbb4501c https://nrc-publications.canada.ca/fra/voir/objet/?id=25d67768-c808-4c0f-88c2-94c5bbb4501c eng eng issn:0165-232X Cold Regions Science and Technology, Volume: 86, Publication date: 2013-11-14, Pages: 55–68 doi:10.1016/j.coldregions.2012.10.013 DGGE Hydrocarbon contamination Hydrocarbon degradation Microbial diversity Nitrate reduction Biodegradation Bioremediation Chemical analysis Electrophoresis Fuel tanks Genes Groundwater pollution Hydrocarbons Manganese Nitrates Petroleum chemistry RNA Hydrochemistry Anoxic conditions Bacterium Community structure Fractured medium Microbial community Reduction Sulfate Canada Canadian Shield Northwest Territories Bacteria (microorganisms) Geobacter Pseudomonas Thiobacillus article 2013 ftnrccanada https://doi.org/10.1016/j.coldregions.2012.10.013 2021-09-01T06:27:43Z There is currently limited scientific data to assess whether groundwater bacterial communities in fractured rock environments can degrade petroleum hydrocarbon plumes in cold regions. The former Colomac Mine is located in the Canadian Shield in the Northwest Territories (mean annual air temperature of -. 5°C) and is currently listed on the Federal Contaminated Sites inventory. Groundwater in fractured rock beneath the former fuel tank-farm at the mine site is contaminated by petroleum hydrocarbons. The objectives of this study were to investigate the bacterial community structure in the groundwater associated with hydrocarbon contamination, and to probe for potential anaerobic microbial processes involved in intrinsic bioremediation. Groundwater monitoring wells previously installed at the site were used to collect samples for chemical, isotopic and microbial analyses. Denaturing gradient gel electrophoresis (DGGE) analysis identified a relatively high bacterial diversity in the least contaminated locations, but as the hydrocarbon contamination increased, bacterial diversity decreased. Sequence analysis of the 16S rRNA gene demonstrated that the bacteria belonged to a wide range of genera, such as Pseudomonas, Thiobacillus, and Geobacter, all of which have been associated with the anaerobic degradation of hydrocarbons. Two putative nitrate-reducing genes were detected in samples with high nitrate reducing activity. Both chemical and microbiological results indicated the presence of microbial anaerobic processes by using nitrate, manganese(IV), ferric iron and sulfate as electron acceptors and suggest that these anaerobic processes play an important role in the biodegradation of dissolved petroleum hydrocarbons in the groundwater at the Colomac site. Our results also revealed that more than one biogeochemical process linked to hydrocarbon degradation could be present in a single borehole and these processes could vary spatially at this site. Erratum published in volume 89, page 48, May 2013. DOI:10.1016/j.coldregions.2013.01.007 Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper Northwest Territories National Research Council Canada: NRC Publications Archive Canada Northwest Territories Cold Regions Science and Technology 86 55 68 |
institution |
Open Polar |
collection |
National Research Council Canada: NRC Publications Archive |
op_collection_id |
ftnrccanada |
language |
English |
topic |
DGGE Hydrocarbon contamination Hydrocarbon degradation Microbial diversity Nitrate reduction Biodegradation Bioremediation Chemical analysis Electrophoresis Fuel tanks Genes Groundwater pollution Hydrocarbons Manganese Nitrates Petroleum chemistry RNA Hydrochemistry Anoxic conditions Bacterium Community structure Fractured medium Microbial community Reduction Sulfate Canada Canadian Shield Northwest Territories Bacteria (microorganisms) Geobacter Pseudomonas Thiobacillus |
spellingShingle |
DGGE Hydrocarbon contamination Hydrocarbon degradation Microbial diversity Nitrate reduction Biodegradation Bioremediation Chemical analysis Electrophoresis Fuel tanks Genes Groundwater pollution Hydrocarbons Manganese Nitrates Petroleum chemistry RNA Hydrochemistry Anoxic conditions Bacterium Community structure Fractured medium Microbial community Reduction Sulfate Canada Canadian Shield Northwest Territories Bacteria (microorganisms) Geobacter Pseudomonas Thiobacillus Yeung, C. William Van Stempvoort, Dale R. Spoelstra, John Bickerton, Greg Voralek, John Greer, Charles W. Bacterial community evidence for anaerobic degradation of petroleum hydrocarbons in cold climate groundwater |
topic_facet |
DGGE Hydrocarbon contamination Hydrocarbon degradation Microbial diversity Nitrate reduction Biodegradation Bioremediation Chemical analysis Electrophoresis Fuel tanks Genes Groundwater pollution Hydrocarbons Manganese Nitrates Petroleum chemistry RNA Hydrochemistry Anoxic conditions Bacterium Community structure Fractured medium Microbial community Reduction Sulfate Canada Canadian Shield Northwest Territories Bacteria (microorganisms) Geobacter Pseudomonas Thiobacillus |
description |
There is currently limited scientific data to assess whether groundwater bacterial communities in fractured rock environments can degrade petroleum hydrocarbon plumes in cold regions. The former Colomac Mine is located in the Canadian Shield in the Northwest Territories (mean annual air temperature of -. 5°C) and is currently listed on the Federal Contaminated Sites inventory. Groundwater in fractured rock beneath the former fuel tank-farm at the mine site is contaminated by petroleum hydrocarbons. The objectives of this study were to investigate the bacterial community structure in the groundwater associated with hydrocarbon contamination, and to probe for potential anaerobic microbial processes involved in intrinsic bioremediation. Groundwater monitoring wells previously installed at the site were used to collect samples for chemical, isotopic and microbial analyses. Denaturing gradient gel electrophoresis (DGGE) analysis identified a relatively high bacterial diversity in the least contaminated locations, but as the hydrocarbon contamination increased, bacterial diversity decreased. Sequence analysis of the 16S rRNA gene demonstrated that the bacteria belonged to a wide range of genera, such as Pseudomonas, Thiobacillus, and Geobacter, all of which have been associated with the anaerobic degradation of hydrocarbons. Two putative nitrate-reducing genes were detected in samples with high nitrate reducing activity. Both chemical and microbiological results indicated the presence of microbial anaerobic processes by using nitrate, manganese(IV), ferric iron and sulfate as electron acceptors and suggest that these anaerobic processes play an important role in the biodegradation of dissolved petroleum hydrocarbons in the groundwater at the Colomac site. Our results also revealed that more than one biogeochemical process linked to hydrocarbon degradation could be present in a single borehole and these processes could vary spatially at this site. Erratum published in volume 89, page 48, May 2013. DOI:10.1016/j.coldregions.2013.01.007 Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Yeung, C. William Van Stempvoort, Dale R. Spoelstra, John Bickerton, Greg Voralek, John Greer, Charles W. |
author_facet |
Yeung, C. William Van Stempvoort, Dale R. Spoelstra, John Bickerton, Greg Voralek, John Greer, Charles W. |
author_sort |
Yeung, C. William |
title |
Bacterial community evidence for anaerobic degradation of petroleum hydrocarbons in cold climate groundwater |
title_short |
Bacterial community evidence for anaerobic degradation of petroleum hydrocarbons in cold climate groundwater |
title_full |
Bacterial community evidence for anaerobic degradation of petroleum hydrocarbons in cold climate groundwater |
title_fullStr |
Bacterial community evidence for anaerobic degradation of petroleum hydrocarbons in cold climate groundwater |
title_full_unstemmed |
Bacterial community evidence for anaerobic degradation of petroleum hydrocarbons in cold climate groundwater |
title_sort |
bacterial community evidence for anaerobic degradation of petroleum hydrocarbons in cold climate groundwater |
publishDate |
2013 |
url |
https://doi.org/10.1016/j.coldregions.2012.10.013 https://nrc-publications.canada.ca/eng/view/object/?id=25d67768-c808-4c0f-88c2-94c5bbb4501c https://nrc-publications.canada.ca/fra/voir/objet/?id=25d67768-c808-4c0f-88c2-94c5bbb4501c |
geographic |
Canada Northwest Territories |
geographic_facet |
Canada Northwest Territories |
genre |
Northwest Territories |
genre_facet |
Northwest Territories |
op_relation |
issn:0165-232X Cold Regions Science and Technology, Volume: 86, Publication date: 2013-11-14, Pages: 55–68 doi:10.1016/j.coldregions.2012.10.013 |
op_doi |
https://doi.org/10.1016/j.coldregions.2012.10.013 |
container_title |
Cold Regions Science and Technology |
container_volume |
86 |
container_start_page |
55 |
op_container_end_page |
68 |
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1766150424443748352 |