Hydrocarbon-degrading potential of microbial communities from Arctic plants

• Published in: Journal of Applied Microbiology
• Main Authors: Ferrera-Rodríguez, O., Greer, C.W., Juck, D., Consaul, L.L., Martínez-Romero, E., Whyte, L.G.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2013
• Subjects: hydrocarbon; RNA 16S; arctic environment; bacterium; biodegradation; microbial community; phytoremediation; rhizosphere; soil microorganism; taxonomy; Actinobacteria; Arctic plant; bacterial count; bacterial gene; Bacteroidetes; Firmicutes; gene sequence; incubation temperature; Mycobacterium; nucleotide sequence; phylogeny; plant; Proteobacteria; Pseudomonas; Canada; Canadian Arctic; Ellesmere Island; Eureka; Nunavut; Queen Elizabeth Islands; Arthrobacter; Bacteria (microorganisms); Eriophorum scheuchzeri; Intrasporangiaceae; Nocardia; Oxyria digyna; Potentilla rubricaulis; Puccinellia angustata; Rhodococcus; Salix arctica; Arctic; Eriophorum
• Online Access: https://doi.org/10.1111/jam.12020; https://nrc-publications.canada.ca/eng/view/object/?id=fa0c9b98-f435-4090-aaa3-364df781956e; https://nrc-publications.canada.ca/fra/voir/objet/?id=fa0c9b98-f435-4090-aaa3-364df781956e

Aims: To explore rhizospheric microbial communities from Arctic native plant species evaluating their bacterial hydrocarbon-degrading capacities. Methods and Results: Eriophorum scheuchzeri, Potentilla cf. rubricaulis, Oxyria digyna, Salix arctica and Puccinellia angustata plant species were collected at Eureka (Canadian high Arctic) along with their rhizospheric soil samples. Their bacterial community fingerprints (16S rRNA gene, DGGE) were distinctive encompassing members from the phyla: Bacteroidetes, Firmicutes, Actinobacteria and Proteobacteria. Isolated diesel-degrading bacteria belonged to the phyla Actinobacteria and Proteobacteria. Strains of Mycobacterium, Nocardia, Rhodococcus, Intrasporangiaceae, Leifsoni and Arthrobacter possessed alkB and Pseudomonas possessed both ndoB and xylE gene sequences. Two Rhodococcus strains mineralized [1-14C] hexadecane at 5 and -5°C. From the rhizosphere of P. angustata, larger numbers of hydrocarbon-degrading bacteria were isolated than from other plant rhizosphere samples and all three genes alkB (from Actinobacteria), ndoB and xylE (from Pseudomonas) were detected by PCR. Conclusions: (i) Arctic plants have unique rhizospheric bacterial communities. (ii) P. angustata has potential for phytoremediation research at high Arctic soils. (iii) Isolated bacteria mineralized hydrocarbons at ambient low temperatures. Significance and Impact of the Study: To the best of our knowledge, this is the first rhizospheric exploration examining the phytoremediation potential of five Arctic plants and evaluating their microbial hydrocarbon-degrading capacities. © 2012 The Society for Applied Microbiology. Peer reviewed: Yes NRC publication: Yes