Capillary electrophoresis mass spectrometry for the characterization of O-acetylated N-glycans from fish serum
O-Acetylated N-glycans from fish serum of Atlantic salmon (Salmo salar) are characterized by capillary electrophoresis (CE) in conjunction with both laser-induced fluorescence (LIF) and mass spectrometry (MS) detection methods. Glycans derivatized with negatively charged fluorescent label 8-aminopyr...
Published in: | Analytical Chemistry |
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2012
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:21268858 2023-05-15T15:32:24+02:00 Capillary electrophoresis mass spectrometry for the characterization of O-acetylated N-glycans from fish serum Jayo, Roxana G. Li, Jianjun Chen, David D. Y. 2012-10-16 text https://doi.org/10.1021/ac301889k https://nrc-publications.canada.ca/eng/view/object/?id=581586a7-a076-4e6d-ae94-32cfa1f9264d https://nrc-publications.canada.ca/fra/voir/objet/?id=581586a7-a076-4e6d-ae94-32cfa1f9264d eng eng issn:0003-2700 issn:1520-6882 Analytical Chemistry, Volume: 84, Issue: 20, Publication date: 2012-10-16, Pages: 8756–8762 doi:10.1021/ac301889k article 2012 ftnrccanada https://doi.org/10.1021/ac301889k 2021-09-01T06:26:33Z O-Acetylated N-glycans from fish serum of Atlantic salmon (Salmo salar) are characterized by capillary electrophoresis (CE) in conjunction with both laser-induced fluorescence (LIF) and mass spectrometry (MS) detection methods. Glycans derivatized with negatively charged fluorescent label 8-aminopyrene-1,2,6-trisulfonate (APTS) were separated to obtain a CE-LIF profile of the complex glycan mixture, and the profile concurs with that obtained by using electrospray mass spectrometry. The identity of the APTS-labeled glycans was confirmed by CE–MS. The same glycans can be identified also in their native state by CE–MS without derivatization. The structural variations of O-acetylated sialic acid isomers in fish serum glycans are investigated by CE–MS/MS. Selected ion monitoring provided useful structural information of the underivatized glycans from fragmentation spectra. New complex fish serum glycans that are not reported previously were observed and characterized. These methods may be useful not only for the characterization of acetylation of complex glycans but also to study other types of glycan modifications, as well as to allow determination of overall glycan composition in glycoproteins. Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper Atlantic salmon Salmo salar National Research Council Canada: NRC Publications Archive Analytical Chemistry 84 20 8756 8762 |
institution |
Open Polar |
collection |
National Research Council Canada: NRC Publications Archive |
op_collection_id |
ftnrccanada |
language |
English |
description |
O-Acetylated N-glycans from fish serum of Atlantic salmon (Salmo salar) are characterized by capillary electrophoresis (CE) in conjunction with both laser-induced fluorescence (LIF) and mass spectrometry (MS) detection methods. Glycans derivatized with negatively charged fluorescent label 8-aminopyrene-1,2,6-trisulfonate (APTS) were separated to obtain a CE-LIF profile of the complex glycan mixture, and the profile concurs with that obtained by using electrospray mass spectrometry. The identity of the APTS-labeled glycans was confirmed by CE–MS. The same glycans can be identified also in their native state by CE–MS without derivatization. The structural variations of O-acetylated sialic acid isomers in fish serum glycans are investigated by CE–MS/MS. Selected ion monitoring provided useful structural information of the underivatized glycans from fragmentation spectra. New complex fish serum glycans that are not reported previously were observed and characterized. These methods may be useful not only for the characterization of acetylation of complex glycans but also to study other types of glycan modifications, as well as to allow determination of overall glycan composition in glycoproteins. Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Jayo, Roxana G. Li, Jianjun Chen, David D. Y. |
spellingShingle |
Jayo, Roxana G. Li, Jianjun Chen, David D. Y. Capillary electrophoresis mass spectrometry for the characterization of O-acetylated N-glycans from fish serum |
author_facet |
Jayo, Roxana G. Li, Jianjun Chen, David D. Y. |
author_sort |
Jayo, Roxana G. |
title |
Capillary electrophoresis mass spectrometry for the characterization of O-acetylated N-glycans from fish serum |
title_short |
Capillary electrophoresis mass spectrometry for the characterization of O-acetylated N-glycans from fish serum |
title_full |
Capillary electrophoresis mass spectrometry for the characterization of O-acetylated N-glycans from fish serum |
title_fullStr |
Capillary electrophoresis mass spectrometry for the characterization of O-acetylated N-glycans from fish serum |
title_full_unstemmed |
Capillary electrophoresis mass spectrometry for the characterization of O-acetylated N-glycans from fish serum |
title_sort |
capillary electrophoresis mass spectrometry for the characterization of o-acetylated n-glycans from fish serum |
publishDate |
2012 |
url |
https://doi.org/10.1021/ac301889k https://nrc-publications.canada.ca/eng/view/object/?id=581586a7-a076-4e6d-ae94-32cfa1f9264d https://nrc-publications.canada.ca/fra/voir/objet/?id=581586a7-a076-4e6d-ae94-32cfa1f9264d |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
issn:0003-2700 issn:1520-6882 Analytical Chemistry, Volume: 84, Issue: 20, Publication date: 2012-10-16, Pages: 8756–8762 doi:10.1021/ac301889k |
op_doi |
https://doi.org/10.1021/ac301889k |
container_title |
Analytical Chemistry |
container_volume |
84 |
container_issue |
20 |
container_start_page |
8756 |
op_container_end_page |
8762 |
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1766362909069279232 |