Petroleum hydrocarbon biodegradation under seasonal freeze-thaw soil temperature regimes in contaminated soils from a sub-Arctic site
Several studies have shown that biostimulation in ex situ systems such as landfarms and biopiles can facilitate remediation of petroleum hydrocarbon contaminated soils at sub-Arctic sites during summers when temperatures are above freezing. In this study, we examine the biodegradation of semivolatil...
Published in: | Environmental Science & Technology |
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Language: | English |
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2011
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Online Access: | https://doi.org/10.1021/es1022653 https://nrc-publications.canada.ca/eng/view/accepted/?id=67ad34a4-a4e5-4675-8232-ccf2b71082e0 https://nrc-publications.canada.ca/eng/view/object/?id=67ad34a4-a4e5-4675-8232-ccf2b71082e0 https://nrc-publications.canada.ca/fra/voir/objet/?id=67ad34a4-a4e5-4675-8232-ccf2b71082e0 |
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:16966054 2023-05-15T14:55:47+02:00 Petroleum hydrocarbon biodegradation under seasonal freeze-thaw soil temperature regimes in contaminated soils from a sub-Arctic site Chang, Wonjae Klemm, Sara Beaulieu, Chantale Hawari, Jalal Whyte, Lyle Ghoshal, Subhasis 2011-02-01 text https://doi.org/10.1021/es1022653 https://nrc-publications.canada.ca/eng/view/accepted/?id=67ad34a4-a4e5-4675-8232-ccf2b71082e0 https://nrc-publications.canada.ca/eng/view/object/?id=67ad34a4-a4e5-4675-8232-ccf2b71082e0 https://nrc-publications.canada.ca/fra/voir/objet/?id=67ad34a4-a4e5-4675-8232-ccf2b71082e0 eng eng Environmental Science & Technology, Volume: 45, Issue: 3, Publication date: 2011-02-01, Pages: 1061–1066 doi:10.1021/es1022653 article 2011 ftnrccanada https://doi.org/10.1021/es1022653 2021-09-01T06:26:07Z Several studies have shown that biostimulation in ex situ systems such as landfarms and biopiles can facilitate remediation of petroleum hydrocarbon contaminated soils at sub-Arctic sites during summers when temperatures are above freezing. In this study, we examine the biodegradation of semivolatile (F2: C10-C16) and nonvolatile (F3: C16-C34) petroleum hydrocarbons and microbial respiration and population dynamics at post- and presummer temperatures ranging from -5 to 14 °C. The studies were conducted in pilot-scale tanks with soils obtained from a historically contaminated sub-Arctic site in Resolution Island (RI), Canada. In aerobic, nutrient-amended, unsaturated soils, the F2 hydrocarbons decreased by 32% during the seasonal freeze-thaw phase where soils were cooled from 2 to -5 °C at a freezing rate of -0.12 °C d-1 and then thawed from -5 to 4 °C at a thawing rate of +0.16 °C d-1. In the unamended (control) tank, the F2 fraction only decreased by 14% during the same period. Biodegradation of individual hydrocarbon compounds in the nutrient-amended soils was also confirmed by comparing their abundance over time to that of the conserved diesel biomarker, bicyclic sesquiterpanes (BS). During this period, microbial respiration was observed, even at subzero temperatures when unfrozen liquid water was detected during the freeze-thaw period. An increase in culturable heterotrophs and 16S rDNA copy numbers was noted during the freezing phase, and the 14C-hexadecane mineralization in soil samples obtained from the nutrient-amended tank steadily increased. Hydrocarbon degrading bacterial populations identified as Corynebacterineae- and Alkanindiges-related strains emerged during the freezing and thawing phases, respectively, indicating there were temperature-based microbial community shifts. Peer reviewed: Yes NRC publication: Yes Article in Journal/Newspaper Arctic National Research Council Canada: NRC Publications Archive Arctic Canada Resolution Island ENVELOPE(-64.981,-64.981,61.551,61.551) Environmental Science & Technology 45 3 1061 1066 |
institution |
Open Polar |
collection |
National Research Council Canada: NRC Publications Archive |
op_collection_id |
ftnrccanada |
language |
English |
description |
Several studies have shown that biostimulation in ex situ systems such as landfarms and biopiles can facilitate remediation of petroleum hydrocarbon contaminated soils at sub-Arctic sites during summers when temperatures are above freezing. In this study, we examine the biodegradation of semivolatile (F2: C10-C16) and nonvolatile (F3: C16-C34) petroleum hydrocarbons and microbial respiration and population dynamics at post- and presummer temperatures ranging from -5 to 14 °C. The studies were conducted in pilot-scale tanks with soils obtained from a historically contaminated sub-Arctic site in Resolution Island (RI), Canada. In aerobic, nutrient-amended, unsaturated soils, the F2 hydrocarbons decreased by 32% during the seasonal freeze-thaw phase where soils were cooled from 2 to -5 °C at a freezing rate of -0.12 °C d-1 and then thawed from -5 to 4 °C at a thawing rate of +0.16 °C d-1. In the unamended (control) tank, the F2 fraction only decreased by 14% during the same period. Biodegradation of individual hydrocarbon compounds in the nutrient-amended soils was also confirmed by comparing their abundance over time to that of the conserved diesel biomarker, bicyclic sesquiterpanes (BS). During this period, microbial respiration was observed, even at subzero temperatures when unfrozen liquid water was detected during the freeze-thaw period. An increase in culturable heterotrophs and 16S rDNA copy numbers was noted during the freezing phase, and the 14C-hexadecane mineralization in soil samples obtained from the nutrient-amended tank steadily increased. Hydrocarbon degrading bacterial populations identified as Corynebacterineae- and Alkanindiges-related strains emerged during the freezing and thawing phases, respectively, indicating there were temperature-based microbial community shifts. Peer reviewed: Yes NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Chang, Wonjae Klemm, Sara Beaulieu, Chantale Hawari, Jalal Whyte, Lyle Ghoshal, Subhasis |
spellingShingle |
Chang, Wonjae Klemm, Sara Beaulieu, Chantale Hawari, Jalal Whyte, Lyle Ghoshal, Subhasis Petroleum hydrocarbon biodegradation under seasonal freeze-thaw soil temperature regimes in contaminated soils from a sub-Arctic site |
author_facet |
Chang, Wonjae Klemm, Sara Beaulieu, Chantale Hawari, Jalal Whyte, Lyle Ghoshal, Subhasis |
author_sort |
Chang, Wonjae |
title |
Petroleum hydrocarbon biodegradation under seasonal freeze-thaw soil temperature regimes in contaminated soils from a sub-Arctic site |
title_short |
Petroleum hydrocarbon biodegradation under seasonal freeze-thaw soil temperature regimes in contaminated soils from a sub-Arctic site |
title_full |
Petroleum hydrocarbon biodegradation under seasonal freeze-thaw soil temperature regimes in contaminated soils from a sub-Arctic site |
title_fullStr |
Petroleum hydrocarbon biodegradation under seasonal freeze-thaw soil temperature regimes in contaminated soils from a sub-Arctic site |
title_full_unstemmed |
Petroleum hydrocarbon biodegradation under seasonal freeze-thaw soil temperature regimes in contaminated soils from a sub-Arctic site |
title_sort |
petroleum hydrocarbon biodegradation under seasonal freeze-thaw soil temperature regimes in contaminated soils from a sub-arctic site |
publishDate |
2011 |
url |
https://doi.org/10.1021/es1022653 https://nrc-publications.canada.ca/eng/view/accepted/?id=67ad34a4-a4e5-4675-8232-ccf2b71082e0 https://nrc-publications.canada.ca/eng/view/object/?id=67ad34a4-a4e5-4675-8232-ccf2b71082e0 https://nrc-publications.canada.ca/fra/voir/objet/?id=67ad34a4-a4e5-4675-8232-ccf2b71082e0 |
long_lat |
ENVELOPE(-64.981,-64.981,61.551,61.551) |
geographic |
Arctic Canada Resolution Island |
geographic_facet |
Arctic Canada Resolution Island |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
Environmental Science & Technology, Volume: 45, Issue: 3, Publication date: 2011-02-01, Pages: 1061–1066 doi:10.1021/es1022653 |
op_doi |
https://doi.org/10.1021/es1022653 |
container_title |
Environmental Science & Technology |
container_volume |
45 |
container_issue |
3 |
container_start_page |
1061 |
op_container_end_page |
1066 |
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1766327799553982464 |