Heterotrophic and autotrophic microbial populations in cold perennial springs of the High Arctic
The saline springs of Gypsum Hill in the Canadian high Arctic are a rare example of cold springs originating from deep groundwater and rising to the surface through thick permafrost. The heterotrophic bacteria and autotrophic sulfur-oxidizing bacteria (up to 40% of the total microbial community) iso...
Published in: | Applied and Environmental Microbiology |
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2008
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Online Access: | https://doi.org/10.1128/AEM.00359-08 https://nrc-publications.canada.ca/eng/view/accepted/?id=576c6658-5f63-4101-aa63-376d526131a7 https://nrc-publications.canada.ca/eng/view/object/?id=576c6658-5f63-4101-aa63-376d526131a7 https://nrc-publications.canada.ca/fra/voir/objet/?id=576c6658-5f63-4101-aa63-376d526131a7 |
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:12441158 2023-05-15T14:53:37+02:00 Heterotrophic and autotrophic microbial populations in cold perennial springs of the High Arctic Perreault, Nancy N. Greer, Charles W. Andersen, Dale T. Tille, Stefanie Lacrampe-Couloume, Georges Sherwood Lollar, Barbara Whyte, Lyle G. 2008-11 text https://doi.org/10.1128/AEM.00359-08 https://nrc-publications.canada.ca/eng/view/accepted/?id=576c6658-5f63-4101-aa63-376d526131a7 https://nrc-publications.canada.ca/eng/view/object/?id=576c6658-5f63-4101-aa63-376d526131a7 https://nrc-publications.canada.ca/fra/voir/objet/?id=576c6658-5f63-4101-aa63-376d526131a7 eng eng Applied and Environmental Microbiology, Volume: 74, Issue: 22, Publication date: 2008-11, Pages: 6898–6907 doi:10.1128/AEM.00359-08 environmental article 2008 ftnrccanada https://doi.org/10.1128/AEM.00359-08 2021-09-01T06:22:14Z The saline springs of Gypsum Hill in the Canadian high Arctic are a rare example of cold springs originating from deep groundwater and rising to the surface through thick permafrost. The heterotrophic bacteria and autotrophic sulfur-oxidizing bacteria (up to 40% of the total microbial community) isolated from the spring waters and sediments were classified into four phyla (Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria) based on 16S rRNA gene analysis; heterotrophic isolates were primarily psychrotolerant, salt-tolerant, facultative anaerobes. Some of the isolates contained genes for thiosulfate oxidation (soxB) and anoxygenic photosynthesis (pufM), possibly enabling the strains to better compete in these sulfur-rich environments subject to long periods of illumination in the Arctic summer. Although leucine uptake by the spring water microbial community was low, CO2 uptake was relatively high under dark incubation, reinforcing the idea that primary production by chemoautotrophs is an important process in the springs. The small amounts of hydrocarbons in gases exsolving from the springs (0.38 to 0.51% CH4) were compositionally and isotopically consistent with microbial methanogenesis and possible methanotrophy. Anaerobic heterotrophic sulfur oxidation and aerobic autotrophic sulfur oxidation activities were demonstrated in sediment slurries. Overall, our results describe an active microbial community capable of sustainability in an extreme environment that experiences prolonged periods of continuous light or darkness, low temperatures, and moderate salinity, where life seems to rely on chemolithoautotrophy. NRC publication: Yes Article in Journal/Newspaper Arctic permafrost National Research Council Canada: NRC Publications Archive Arctic Gypsum Hill ENVELOPE(-90.751,-90.751,79.402,79.402) Applied and Environmental Microbiology 74 22 6898 6907 |
institution |
Open Polar |
collection |
National Research Council Canada: NRC Publications Archive |
op_collection_id |
ftnrccanada |
language |
English |
topic |
environmental |
spellingShingle |
environmental Perreault, Nancy N. Greer, Charles W. Andersen, Dale T. Tille, Stefanie Lacrampe-Couloume, Georges Sherwood Lollar, Barbara Whyte, Lyle G. Heterotrophic and autotrophic microbial populations in cold perennial springs of the High Arctic |
topic_facet |
environmental |
description |
The saline springs of Gypsum Hill in the Canadian high Arctic are a rare example of cold springs originating from deep groundwater and rising to the surface through thick permafrost. The heterotrophic bacteria and autotrophic sulfur-oxidizing bacteria (up to 40% of the total microbial community) isolated from the spring waters and sediments were classified into four phyla (Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria) based on 16S rRNA gene analysis; heterotrophic isolates were primarily psychrotolerant, salt-tolerant, facultative anaerobes. Some of the isolates contained genes for thiosulfate oxidation (soxB) and anoxygenic photosynthesis (pufM), possibly enabling the strains to better compete in these sulfur-rich environments subject to long periods of illumination in the Arctic summer. Although leucine uptake by the spring water microbial community was low, CO2 uptake was relatively high under dark incubation, reinforcing the idea that primary production by chemoautotrophs is an important process in the springs. The small amounts of hydrocarbons in gases exsolving from the springs (0.38 to 0.51% CH4) were compositionally and isotopically consistent with microbial methanogenesis and possible methanotrophy. Anaerobic heterotrophic sulfur oxidation and aerobic autotrophic sulfur oxidation activities were demonstrated in sediment slurries. Overall, our results describe an active microbial community capable of sustainability in an extreme environment that experiences prolonged periods of continuous light or darkness, low temperatures, and moderate salinity, where life seems to rely on chemolithoautotrophy. NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Perreault, Nancy N. Greer, Charles W. Andersen, Dale T. Tille, Stefanie Lacrampe-Couloume, Georges Sherwood Lollar, Barbara Whyte, Lyle G. |
author_facet |
Perreault, Nancy N. Greer, Charles W. Andersen, Dale T. Tille, Stefanie Lacrampe-Couloume, Georges Sherwood Lollar, Barbara Whyte, Lyle G. |
author_sort |
Perreault, Nancy N. |
title |
Heterotrophic and autotrophic microbial populations in cold perennial springs of the High Arctic |
title_short |
Heterotrophic and autotrophic microbial populations in cold perennial springs of the High Arctic |
title_full |
Heterotrophic and autotrophic microbial populations in cold perennial springs of the High Arctic |
title_fullStr |
Heterotrophic and autotrophic microbial populations in cold perennial springs of the High Arctic |
title_full_unstemmed |
Heterotrophic and autotrophic microbial populations in cold perennial springs of the High Arctic |
title_sort |
heterotrophic and autotrophic microbial populations in cold perennial springs of the high arctic |
publishDate |
2008 |
url |
https://doi.org/10.1128/AEM.00359-08 https://nrc-publications.canada.ca/eng/view/accepted/?id=576c6658-5f63-4101-aa63-376d526131a7 https://nrc-publications.canada.ca/eng/view/object/?id=576c6658-5f63-4101-aa63-376d526131a7 https://nrc-publications.canada.ca/fra/voir/objet/?id=576c6658-5f63-4101-aa63-376d526131a7 |
long_lat |
ENVELOPE(-90.751,-90.751,79.402,79.402) |
geographic |
Arctic Gypsum Hill |
geographic_facet |
Arctic Gypsum Hill |
genre |
Arctic permafrost |
genre_facet |
Arctic permafrost |
op_relation |
Applied and Environmental Microbiology, Volume: 74, Issue: 22, Publication date: 2008-11, Pages: 6898–6907 doi:10.1128/AEM.00359-08 |
op_doi |
https://doi.org/10.1128/AEM.00359-08 |
container_title |
Applied and Environmental Microbiology |
container_volume |
74 |
container_issue |
22 |
container_start_page |
6898 |
op_container_end_page |
6907 |
_version_ |
1766325226971332608 |