Utilization of Fluorescent Microspheres and a Green Fluorescent Protein-Marked Strain for Assessment of Microbiological Contamination of Permafrost and Ground Ice Core Samples from the Canadian High Arctic
Fluorescent microspheres were applied in a novel fashion during subsurface drilling of permafrost and ground ice in the Canadian High Arctic to monitor the exogenous microbiological contamination of core samples obtained during the drilling process. Prior to each drill run, a concentrated fluorescen...
Published in: | Applied and Environmental Microbiology |
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Online Access: | https://doi.org/10.1128/AEM.71.2.1035-1041.2005 https://nrc-publications.canada.ca/eng/view/object/?id=bb14390d-f2b1-44dd-baf8-0127785080a7 https://nrc-publications.canada.ca/fra/voir/objet/?id=bb14390d-f2b1-44dd-baf8-0127785080a7 |
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ftnrccanada:oai:cisti-icist.nrc-cnrc.ca:cistinparc:12332839 2023-05-15T14:57:51+02:00 Utilization of Fluorescent Microspheres and a Green Fluorescent Protein-Marked Strain for Assessment of Microbiological Contamination of Permafrost and Ground Ice Core Samples from the Canadian High Arctic Juck, David Whissell, G. Steven, B. Pollard, W. McKay, Craig Greer, C. Whyte, L. 2005-02-01 text https://doi.org/10.1128/AEM.71.2.1035-1041.2005 https://nrc-publications.canada.ca/eng/view/object/?id=bb14390d-f2b1-44dd-baf8-0127785080a7 https://nrc-publications.canada.ca/fra/voir/objet/?id=bb14390d-f2b1-44dd-baf8-0127785080a7 unknown Acoustics, Speech, and Signal Processing Newsletter, IEEE, Volume: 71, Issue: 2, Publication date: 2005-02-01, Pages: 1035–1041 doi:10.1128/AEM.71.2.1035-1041.2005 article 2005 ftnrccanada https://doi.org/10.1128/AEM.71.2.1035-1041.2005 2021-09-01T06:21:42Z Fluorescent microspheres were applied in a novel fashion during subsurface drilling of permafrost and ground ice in the Canadian High Arctic to monitor the exogenous microbiological contamination of core samples obtained during the drilling process. Prior to each drill run, a concentrated fluorescent microsphere (0.5-{micro}m diameter) solution was applied to the interior surfaces of the drill bit, core catcher, and core tube and allowed to dry. Macroscopic examination in the field demonstrated reliable transfer of the microspheres to core samples, while detailed microscopic examination revealed penetration levels of less than 1 cm from the core exterior. To monitor for microbial contamination during downstream processing of the permafrost and ground ice cores, a Pseudomonas strain expressing the green fluorescent protein (GFP) was painted on the core exterior prior to processing. Contamination of the processed core interiors with the GFP-expressing strain was not detected by culturing the samples or by PCR to detect the gfp marker gene. These methodologies were quick, were easy to apply, and should help to monitor the exogenous microbiological contamination of pristine permafrost and ground ice samples for downstream culture-dependent and culture-independent microbial analyses. NRC publication: Yes Article in Journal/Newspaper Arctic Ice ice core permafrost National Research Council Canada: NRC Publications Archive Arctic Applied and Environmental Microbiology 71 2 1035 1041 |
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Open Polar |
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National Research Council Canada: NRC Publications Archive |
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ftnrccanada |
language |
unknown |
description |
Fluorescent microspheres were applied in a novel fashion during subsurface drilling of permafrost and ground ice in the Canadian High Arctic to monitor the exogenous microbiological contamination of core samples obtained during the drilling process. Prior to each drill run, a concentrated fluorescent microsphere (0.5-{micro}m diameter) solution was applied to the interior surfaces of the drill bit, core catcher, and core tube and allowed to dry. Macroscopic examination in the field demonstrated reliable transfer of the microspheres to core samples, while detailed microscopic examination revealed penetration levels of less than 1 cm from the core exterior. To monitor for microbial contamination during downstream processing of the permafrost and ground ice cores, a Pseudomonas strain expressing the green fluorescent protein (GFP) was painted on the core exterior prior to processing. Contamination of the processed core interiors with the GFP-expressing strain was not detected by culturing the samples or by PCR to detect the gfp marker gene. These methodologies were quick, were easy to apply, and should help to monitor the exogenous microbiological contamination of pristine permafrost and ground ice samples for downstream culture-dependent and culture-independent microbial analyses. NRC publication: Yes |
format |
Article in Journal/Newspaper |
author |
Juck, David Whissell, G. Steven, B. Pollard, W. McKay, Craig Greer, C. Whyte, L. |
spellingShingle |
Juck, David Whissell, G. Steven, B. Pollard, W. McKay, Craig Greer, C. Whyte, L. Utilization of Fluorescent Microspheres and a Green Fluorescent Protein-Marked Strain for Assessment of Microbiological Contamination of Permafrost and Ground Ice Core Samples from the Canadian High Arctic |
author_facet |
Juck, David Whissell, G. Steven, B. Pollard, W. McKay, Craig Greer, C. Whyte, L. |
author_sort |
Juck, David |
title |
Utilization of Fluorescent Microspheres and a Green Fluorescent Protein-Marked Strain for Assessment of Microbiological Contamination of Permafrost and Ground Ice Core Samples from the Canadian High Arctic |
title_short |
Utilization of Fluorescent Microspheres and a Green Fluorescent Protein-Marked Strain for Assessment of Microbiological Contamination of Permafrost and Ground Ice Core Samples from the Canadian High Arctic |
title_full |
Utilization of Fluorescent Microspheres and a Green Fluorescent Protein-Marked Strain for Assessment of Microbiological Contamination of Permafrost and Ground Ice Core Samples from the Canadian High Arctic |
title_fullStr |
Utilization of Fluorescent Microspheres and a Green Fluorescent Protein-Marked Strain for Assessment of Microbiological Contamination of Permafrost and Ground Ice Core Samples from the Canadian High Arctic |
title_full_unstemmed |
Utilization of Fluorescent Microspheres and a Green Fluorescent Protein-Marked Strain for Assessment of Microbiological Contamination of Permafrost and Ground Ice Core Samples from the Canadian High Arctic |
title_sort |
utilization of fluorescent microspheres and a green fluorescent protein-marked strain for assessment of microbiological contamination of permafrost and ground ice core samples from the canadian high arctic |
publishDate |
2005 |
url |
https://doi.org/10.1128/AEM.71.2.1035-1041.2005 https://nrc-publications.canada.ca/eng/view/object/?id=bb14390d-f2b1-44dd-baf8-0127785080a7 https://nrc-publications.canada.ca/fra/voir/objet/?id=bb14390d-f2b1-44dd-baf8-0127785080a7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Ice ice core permafrost |
genre_facet |
Arctic Ice ice core permafrost |
op_relation |
Acoustics, Speech, and Signal Processing Newsletter, IEEE, Volume: 71, Issue: 2, Publication date: 2005-02-01, Pages: 1035–1041 doi:10.1128/AEM.71.2.1035-1041.2005 |
op_doi |
https://doi.org/10.1128/AEM.71.2.1035-1041.2005 |
container_title |
Applied and Environmental Microbiology |
container_volume |
71 |
container_issue |
2 |
container_start_page |
1035 |
op_container_end_page |
1041 |
_version_ |
1766329949461938176 |