Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms
The impact of ocean acidification and carbonation on microbial community structure was assessed during a large-scale in situ costal pelagic mesocosm study, included as part of the EPOCA 2010 Arctic campaign. The mesocosm experiment included ambient conditions (fjord) and nine mesocosms, with pCO2 ra...
Published in: | Biogeosciences |
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Online Access: | https://openknowledge.nau.edu/id/eprint/482/ https://openknowledge.nau.edu/id/eprint/482/13/Roy_AS_etal_2013_Ocean_acidificfation_shows_negligible_impacts%281%29.pdf https://doi.org/10.5194/bg-10-555-2013 |
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ftnortharizonaun:oai:openknowledge.nau.edu:482 2024-09-15T18:27:59+00:00 Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms Roy, A.-S. Gibbons, S. M. Schunck, H. Owens, S. Caporaso, J. G. Sperling, M. Nissimov, J. I. Romac, S. Bittner, L. Riebesell, U. LaRoche, J. Gilbert, J. A. 2013 text https://openknowledge.nau.edu/id/eprint/482/ https://openknowledge.nau.edu/id/eprint/482/13/Roy_AS_etal_2013_Ocean_acidificfation_shows_negligible_impacts%281%29.pdf https://doi.org/10.5194/bg-10-555-2013 en eng Copernicus Publications https://openknowledge.nau.edu/id/eprint/482/13/Roy_AS_etal_2013_Ocean_acidificfation_shows_negligible_impacts%281%29.pdf Roy, A.-S. and Gibbons, S. M. and Schunck, H. and Owens, S. and Caporaso, J. G. and Sperling, M. and Nissimov, J. I. and Romac, S. and Bittner, L. and Riebesell, U. and LaRoche, J. and Gilbert, J. A. (2013) Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms. Biogeosciences, 10 (1). pp. 555-566. ISSN 1726-4189 doi:10.5194/bg-10-555-2013 cc_by QH Natural history QH426 Genetics Article PeerReviewed 2013 ftnortharizonaun https://doi.org/10.5194/bg-10-555-2013 2024-07-26T03:02:28Z The impact of ocean acidification and carbonation on microbial community structure was assessed during a large-scale in situ costal pelagic mesocosm study, included as part of the EPOCA 2010 Arctic campaign. The mesocosm experiment included ambient conditions (fjord) and nine mesocosms, with pCO2 range from ~145 to ~1420 μatm. Samples collected at nine time points (t-1, t1, t5, t7, t12, t14, t22, t26 to t28) in seven treatments (ambient fjord (~145), 2×~185, ~270, ~685, ~820, ~1050 μatm) were analysed for "free-living" and "particle associated" microbial community composition using 16S rRNA amplicon sequencing. This high-throughput sequencing analysis produced ~20 000 000 16S rRNA V4 reads, which comprised 7000 OTUs. The main variables structuring these communities were, sample origin (fjord or mesocosms) and the filter size fraction (free-living or particle associated). The community was significantly different between the fjord and both the control and elevated 2 mesocosms (which were not significant different) after nutrients were added to the mesocosms; suggesting that the addition of nutrients is the primary driver of the change in mesocosm community structure. The relative importance of each structuring variable depended greatly on the time at which the community was sampled in relation to the phytoplankton bloom. The size fraction was the second most important factor for community structure; separating free-living from particle-associated bacteria. When free-living and particle-associated bacteria were analysed separately at different time points, the only taxon pCO2 was found to significantly affect were the Gammaproteobacteria after nutrient addition. Finally, pCO2 treatment was found to be significantly correlated (non-linear) with 15 rare taxa, most of which increased in abundance with higher CO2. Article in Journal/Newspaper Ocean acidification Phytoplankton OpenKnowledge@NAU (Northern Arizona University, Flagstaff) Biogeosciences 10 1 555 566 |
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Open Polar |
collection |
OpenKnowledge@NAU (Northern Arizona University, Flagstaff) |
op_collection_id |
ftnortharizonaun |
language |
English |
topic |
QH Natural history QH426 Genetics |
spellingShingle |
QH Natural history QH426 Genetics Roy, A.-S. Gibbons, S. M. Schunck, H. Owens, S. Caporaso, J. G. Sperling, M. Nissimov, J. I. Romac, S. Bittner, L. Riebesell, U. LaRoche, J. Gilbert, J. A. Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms |
topic_facet |
QH Natural history QH426 Genetics |
description |
The impact of ocean acidification and carbonation on microbial community structure was assessed during a large-scale in situ costal pelagic mesocosm study, included as part of the EPOCA 2010 Arctic campaign. The mesocosm experiment included ambient conditions (fjord) and nine mesocosms, with pCO2 range from ~145 to ~1420 μatm. Samples collected at nine time points (t-1, t1, t5, t7, t12, t14, t22, t26 to t28) in seven treatments (ambient fjord (~145), 2×~185, ~270, ~685, ~820, ~1050 μatm) were analysed for "free-living" and "particle associated" microbial community composition using 16S rRNA amplicon sequencing. This high-throughput sequencing analysis produced ~20 000 000 16S rRNA V4 reads, which comprised 7000 OTUs. The main variables structuring these communities were, sample origin (fjord or mesocosms) and the filter size fraction (free-living or particle associated). The community was significantly different between the fjord and both the control and elevated 2 mesocosms (which were not significant different) after nutrients were added to the mesocosms; suggesting that the addition of nutrients is the primary driver of the change in mesocosm community structure. The relative importance of each structuring variable depended greatly on the time at which the community was sampled in relation to the phytoplankton bloom. The size fraction was the second most important factor for community structure; separating free-living from particle-associated bacteria. When free-living and particle-associated bacteria were analysed separately at different time points, the only taxon pCO2 was found to significantly affect were the Gammaproteobacteria after nutrient addition. Finally, pCO2 treatment was found to be significantly correlated (non-linear) with 15 rare taxa, most of which increased in abundance with higher CO2. |
format |
Article in Journal/Newspaper |
author |
Roy, A.-S. Gibbons, S. M. Schunck, H. Owens, S. Caporaso, J. G. Sperling, M. Nissimov, J. I. Romac, S. Bittner, L. Riebesell, U. LaRoche, J. Gilbert, J. A. |
author_facet |
Roy, A.-S. Gibbons, S. M. Schunck, H. Owens, S. Caporaso, J. G. Sperling, M. Nissimov, J. I. Romac, S. Bittner, L. Riebesell, U. LaRoche, J. Gilbert, J. A. |
author_sort |
Roy, A.-S. |
title |
Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms |
title_short |
Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms |
title_full |
Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms |
title_fullStr |
Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms |
title_full_unstemmed |
Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms |
title_sort |
ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms |
publisher |
Copernicus Publications |
publishDate |
2013 |
url |
https://openknowledge.nau.edu/id/eprint/482/ https://openknowledge.nau.edu/id/eprint/482/13/Roy_AS_etal_2013_Ocean_acidificfation_shows_negligible_impacts%281%29.pdf https://doi.org/10.5194/bg-10-555-2013 |
genre |
Ocean acidification Phytoplankton |
genre_facet |
Ocean acidification Phytoplankton |
op_relation |
https://openknowledge.nau.edu/id/eprint/482/13/Roy_AS_etal_2013_Ocean_acidificfation_shows_negligible_impacts%281%29.pdf Roy, A.-S. and Gibbons, S. M. and Schunck, H. and Owens, S. and Caporaso, J. G. and Sperling, M. and Nissimov, J. I. and Romac, S. and Bittner, L. and Riebesell, U. and LaRoche, J. and Gilbert, J. A. (2013) Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms. Biogeosciences, 10 (1). pp. 555-566. ISSN 1726-4189 doi:10.5194/bg-10-555-2013 |
op_rights |
cc_by |
op_doi |
https://doi.org/10.5194/bg-10-555-2013 |
container_title |
Biogeosciences |
container_volume |
10 |
container_issue |
1 |
container_start_page |
555 |
op_container_end_page |
566 |
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1810469294152089600 |