Development of species-specific eDNA-based test systems for monitoring of non-indigenous Decapoda in Danish marine waters
Project manager Jesper H. Andersen We report the development of seven eDNA-based species-specific test systems for monitoring of marine Decapoda in Danish marine waters. The seven species are 1) Callinectes sapidus (blå svømmekrabbe), 2) Eriocheir sinensis (kinesisk uldhåndskrabbe), 3) Hemigrapsus s...
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ftnorskinstvf:oai:niva.brage.unit.no:11250/2689446 2024-09-15T18:16:01+00:00 Development of species-specific eDNA-based test systems for monitoring of non-indigenous Decapoda in Danish marine waters Udvikling af artsspecifikke eDNA-baserede testsystemer til overvågning af ikke-hjemmehørende krabber i de danske farvande Knudsen, Steen Wilhelm Møller, Peter Rask 2020 application/pdf https://hdl.handle.net/11250/2689446 eng eng Norsk institutt for vannforskning NIVA-rapport NIVA-rapport;7544 urn:isbn:978-82-577-7279-6 urn:issn:1894-7948 https://hdl.handle.net/11250/2689446 cristin:1849776 © Norsk institutt for vannforskning/Norwegian Institute for Water Research. The publication can be cited freely if the source is stated. 54 7544 Overvåkning Monitoring Havstrategidirektivet Marine Strategy Framework Directive Ikke hjemmehørende arter Non-indigenous species EDNA VDP::Matematikk og naturvitenskap: 400 VDP::Mathematics and natural scienses: 400 Research report 2020 ftnorskinstvf 2024-07-09T03:14:36Z Project manager Jesper H. Andersen We report the development of seven eDNA-based species-specific test systems for monitoring of marine Decapoda in Danish marine waters. The seven species are 1) Callinectes sapidus (blå svømmekrabbe), 2) Eriocheir sinensis (kinesisk uldhåndskrabbe), 3) Hemigrapsus sanguineus (stribet klippekrabbe), 4) Hemigrapsus takanoi (pensel-klippekrabbe), 5) Homarus americanus (amerikansk hummer), 6) Paralithodes camtschaticus (Kamchatka-krabbe) and 7) Rhithropanopeus harrisii (østamerikansk brakvandskrabbe). The following three are new developments: Callinectes sapidus, Hemigrapsus sanguineus and Hemigrapsus takanoi, whilst the remaining four previously developed under earlier phases of the MONIS projects have been tested again. The additional tests on the four previously developed assays were performed with a broader diversity of Decapoda known from Danish seas, to re-evaluate the specificity of these assays. This additional testing revealed that two previously published assays were unspecific, and to accommodate this, two new assays are presented replacing those previously published. We recommend that the species-specific eDNA assays presented here will allow for continuous monitoring of these species as part of the NOVANA monitoring programme. publishedVersion Report Kamchatka Paralithodes camtschaticus Norwegian Institute for Water research: NIVA Open Access Archive (Brage) |
institution |
Open Polar |
collection |
Norwegian Institute for Water research: NIVA Open Access Archive (Brage) |
op_collection_id |
ftnorskinstvf |
language |
English |
topic |
Overvåkning Monitoring Havstrategidirektivet Marine Strategy Framework Directive Ikke hjemmehørende arter Non-indigenous species EDNA VDP::Matematikk og naturvitenskap: 400 VDP::Mathematics and natural scienses: 400 |
spellingShingle |
Overvåkning Monitoring Havstrategidirektivet Marine Strategy Framework Directive Ikke hjemmehørende arter Non-indigenous species EDNA VDP::Matematikk og naturvitenskap: 400 VDP::Mathematics and natural scienses: 400 Knudsen, Steen Wilhelm Møller, Peter Rask Development of species-specific eDNA-based test systems for monitoring of non-indigenous Decapoda in Danish marine waters |
topic_facet |
Overvåkning Monitoring Havstrategidirektivet Marine Strategy Framework Directive Ikke hjemmehørende arter Non-indigenous species EDNA VDP::Matematikk og naturvitenskap: 400 VDP::Mathematics and natural scienses: 400 |
description |
Project manager Jesper H. Andersen We report the development of seven eDNA-based species-specific test systems for monitoring of marine Decapoda in Danish marine waters. The seven species are 1) Callinectes sapidus (blå svømmekrabbe), 2) Eriocheir sinensis (kinesisk uldhåndskrabbe), 3) Hemigrapsus sanguineus (stribet klippekrabbe), 4) Hemigrapsus takanoi (pensel-klippekrabbe), 5) Homarus americanus (amerikansk hummer), 6) Paralithodes camtschaticus (Kamchatka-krabbe) and 7) Rhithropanopeus harrisii (østamerikansk brakvandskrabbe). The following three are new developments: Callinectes sapidus, Hemigrapsus sanguineus and Hemigrapsus takanoi, whilst the remaining four previously developed under earlier phases of the MONIS projects have been tested again. The additional tests on the four previously developed assays were performed with a broader diversity of Decapoda known from Danish seas, to re-evaluate the specificity of these assays. This additional testing revealed that two previously published assays were unspecific, and to accommodate this, two new assays are presented replacing those previously published. We recommend that the species-specific eDNA assays presented here will allow for continuous monitoring of these species as part of the NOVANA monitoring programme. publishedVersion |
format |
Report |
author |
Knudsen, Steen Wilhelm Møller, Peter Rask |
author_facet |
Knudsen, Steen Wilhelm Møller, Peter Rask |
author_sort |
Knudsen, Steen Wilhelm |
title |
Development of species-specific eDNA-based test systems for monitoring of non-indigenous Decapoda in Danish marine waters |
title_short |
Development of species-specific eDNA-based test systems for monitoring of non-indigenous Decapoda in Danish marine waters |
title_full |
Development of species-specific eDNA-based test systems for monitoring of non-indigenous Decapoda in Danish marine waters |
title_fullStr |
Development of species-specific eDNA-based test systems for monitoring of non-indigenous Decapoda in Danish marine waters |
title_full_unstemmed |
Development of species-specific eDNA-based test systems for monitoring of non-indigenous Decapoda in Danish marine waters |
title_sort |
development of species-specific edna-based test systems for monitoring of non-indigenous decapoda in danish marine waters |
publisher |
Norsk institutt for vannforskning |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2689446 |
genre |
Kamchatka Paralithodes camtschaticus |
genre_facet |
Kamchatka Paralithodes camtschaticus |
op_source |
54 7544 |
op_relation |
NIVA-rapport NIVA-rapport;7544 urn:isbn:978-82-577-7279-6 urn:issn:1894-7948 https://hdl.handle.net/11250/2689446 cristin:1849776 |
op_rights |
© Norsk institutt for vannforskning/Norwegian Institute for Water Research. The publication can be cited freely if the source is stated. |
_version_ |
1810453982149083136 |