Dose-dependent hepatic transcriptional responses in Atlantic salmon (Salmo salar) exposed to sublethal doses of gamma radiation
Due to the production of free radicals, gamma radiation may pose a hazard to living organisms. The high-dose radiation effects have been extensively studied, whereas the ecotoxicity data on low-dose gamma radiation is still limited. The present study was therefore performed using Atlantic salmon (Sa...
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Online Access: | http://hdl.handle.net/11250/2566600 https://doi.org/10.1016/j.aquatox.2014.07.021 |
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ftnorskinstvf:oai:niva.brage.unit.no:11250/2566600 2023-05-15T15:31:59+02:00 Dose-dependent hepatic transcriptional responses in Atlantic salmon (Salmo salar) exposed to sublethal doses of gamma radiation Song, You Salbu, Brit Teien, Hans-Christian Heier, Lene Sørlie Rosseland, Bjørn Olav Tollefsen, Knut-Erik 2014 application/pdf http://hdl.handle.net/11250/2566600 https://doi.org/10.1016/j.aquatox.2014.07.021 eng eng Elsevier Norges forskningsråd: 160016 Norges forskningsråd: 178621 Norges forskningsråd: 223268 Aquatic Toxicology. 2014, 156, 52-64. urn:issn:0166-445X http://hdl.handle.net/11250/2566600 https://doi.org/10.1016/j.aquatox.2014.07.021 cristin:1173420 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no CC-BY-NC-ND 52-64 156 Aquatic Toxicology Journal article Peer reviewed 2014 ftnorskinstvf https://doi.org/10.1016/j.aquatox.2014.07.021 2023-02-21T08:46:16Z Due to the production of free radicals, gamma radiation may pose a hazard to living organisms. The high-dose radiation effects have been extensively studied, whereas the ecotoxicity data on low-dose gamma radiation is still limited. The present study was therefore performed using Atlantic salmon (Salmo salar) to characterize effects of low-dose (15, 70 and 280 mGy) gamma radiation after short-term (48 h) exposure. Global transcriptional changes were studied using a combination of high-density oligonucleotide microarrays and quantitative real-time reverse transcription polymerase chain reaction (qPCR). Differentially expressed genes (DEGs; in this article the phrase gene expression is taken as a synonym of gene transcription, although it is acknowledged that gene expression can also be regulated, e.g., at protein stability and translational level) were determined and linked to their biological meanings predicted using both Gene Ontology (GO) and mammalian ortholog-based functional analyses. The plasma glucose level was also measured as a general stress biomarker at the organism level. Results from the microarray analysis revealed a dose-dependent pattern of global transcriptional responses, with 222, 495 and 909 DEGs regulated by 15, 70 and 280 mGy gamma radiation, respectively. Among these DEGs, only 34 were commonly regulated by all radiation doses, whereas the majority of differences were dose-specific. No GO functions were identified at low or medium doses, but repression of DEGs associated with GO functions such as DNA replication, cell cycle regulation and response to reactive oxygen species (ROS) were observed after 280 mGy gamma exposure. Ortholog-based toxicity pathway analysis further showed that 15 mGy radiation affected DEGs associated with cellular signaling and immune response; 70 mGy radiation affected cell cycle regulation and DNA damage repair, cellular energy production; and 280 mGy radiation affected pathways related to cell cycle regulation and DNA repair, mitochondrial dysfunction and immune ... Article in Journal/Newspaper Atlantic salmon Salmo salar Norwegian Institute for Water research: NIVA Open Access Archive (Brage) Aquatic Toxicology 156 52 64 |
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Open Polar |
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Norwegian Institute for Water research: NIVA Open Access Archive (Brage) |
op_collection_id |
ftnorskinstvf |
language |
English |
description |
Due to the production of free radicals, gamma radiation may pose a hazard to living organisms. The high-dose radiation effects have been extensively studied, whereas the ecotoxicity data on low-dose gamma radiation is still limited. The present study was therefore performed using Atlantic salmon (Salmo salar) to characterize effects of low-dose (15, 70 and 280 mGy) gamma radiation after short-term (48 h) exposure. Global transcriptional changes were studied using a combination of high-density oligonucleotide microarrays and quantitative real-time reverse transcription polymerase chain reaction (qPCR). Differentially expressed genes (DEGs; in this article the phrase gene expression is taken as a synonym of gene transcription, although it is acknowledged that gene expression can also be regulated, e.g., at protein stability and translational level) were determined and linked to their biological meanings predicted using both Gene Ontology (GO) and mammalian ortholog-based functional analyses. The plasma glucose level was also measured as a general stress biomarker at the organism level. Results from the microarray analysis revealed a dose-dependent pattern of global transcriptional responses, with 222, 495 and 909 DEGs regulated by 15, 70 and 280 mGy gamma radiation, respectively. Among these DEGs, only 34 were commonly regulated by all radiation doses, whereas the majority of differences were dose-specific. No GO functions were identified at low or medium doses, but repression of DEGs associated with GO functions such as DNA replication, cell cycle regulation and response to reactive oxygen species (ROS) were observed after 280 mGy gamma exposure. Ortholog-based toxicity pathway analysis further showed that 15 mGy radiation affected DEGs associated with cellular signaling and immune response; 70 mGy radiation affected cell cycle regulation and DNA damage repair, cellular energy production; and 280 mGy radiation affected pathways related to cell cycle regulation and DNA repair, mitochondrial dysfunction and immune ... |
format |
Article in Journal/Newspaper |
author |
Song, You Salbu, Brit Teien, Hans-Christian Heier, Lene Sørlie Rosseland, Bjørn Olav Tollefsen, Knut-Erik |
spellingShingle |
Song, You Salbu, Brit Teien, Hans-Christian Heier, Lene Sørlie Rosseland, Bjørn Olav Tollefsen, Knut-Erik Dose-dependent hepatic transcriptional responses in Atlantic salmon (Salmo salar) exposed to sublethal doses of gamma radiation |
author_facet |
Song, You Salbu, Brit Teien, Hans-Christian Heier, Lene Sørlie Rosseland, Bjørn Olav Tollefsen, Knut-Erik |
author_sort |
Song, You |
title |
Dose-dependent hepatic transcriptional responses in Atlantic salmon (Salmo salar) exposed to sublethal doses of gamma radiation |
title_short |
Dose-dependent hepatic transcriptional responses in Atlantic salmon (Salmo salar) exposed to sublethal doses of gamma radiation |
title_full |
Dose-dependent hepatic transcriptional responses in Atlantic salmon (Salmo salar) exposed to sublethal doses of gamma radiation |
title_fullStr |
Dose-dependent hepatic transcriptional responses in Atlantic salmon (Salmo salar) exposed to sublethal doses of gamma radiation |
title_full_unstemmed |
Dose-dependent hepatic transcriptional responses in Atlantic salmon (Salmo salar) exposed to sublethal doses of gamma radiation |
title_sort |
dose-dependent hepatic transcriptional responses in atlantic salmon (salmo salar) exposed to sublethal doses of gamma radiation |
publisher |
Elsevier |
publishDate |
2014 |
url |
http://hdl.handle.net/11250/2566600 https://doi.org/10.1016/j.aquatox.2014.07.021 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
52-64 156 Aquatic Toxicology |
op_relation |
Norges forskningsråd: 160016 Norges forskningsråd: 178621 Norges forskningsråd: 223268 Aquatic Toxicology. 2014, 156, 52-64. urn:issn:0166-445X http://hdl.handle.net/11250/2566600 https://doi.org/10.1016/j.aquatox.2014.07.021 cristin:1173420 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.aquatox.2014.07.021 |
container_title |
Aquatic Toxicology |
container_volume |
156 |
container_start_page |
52 |
op_container_end_page |
64 |
_version_ |
1766362481161142272 |