Primary hepatocytes from Arctic char (Salvelinus alpinus) as a relevant Arctic in vitro model for screening contaminants and environmental extracts
Embargo until 02 April 2019. Contaminants find their way to the Arctic through long-range atmospheric transport, transport via ocean currents, and through increased anthropogenic activity. Some of the typical pollutants reaching the Arctic (PAHs, PCBs) are known to induce cytochrome P450 1a (CYP1A)...
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Online Access: | http://hdl.handle.net/11250/2561365 https://doi.org/10.1016/j.aquatox.2017.03.023 |
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ftnorskinstvf:oai:niva.brage.unit.no:11250/2561365 2023-05-15T14:33:31+02:00 Primary hepatocytes from Arctic char (Salvelinus alpinus) as a relevant Arctic in vitro model for screening contaminants and environmental extracts Petersen, Karina Hultman, Maria T Tollefsen, Knut Erik 2017 application/pdf http://hdl.handle.net/11250/2561365 https://doi.org/10.1016/j.aquatox.2017.03.023 eng eng Elsevier Norges forskningsråd: 221373 Norges forskningsråd: 196318 Aquatic Toxicology. 2017, 187, 141-152. urn:issn:0166-445X http://hdl.handle.net/11250/2561365 https://doi.org/10.1016/j.aquatox.2017.03.023 cristin:1484870 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no CC-BY-NC-ND 141-152 187 Aquatic Toxicology Journal article Peer reviewed 2017 ftnorskinstvf https://doi.org/10.1016/j.aquatox.2017.03.023 2023-02-21T08:45:26Z Embargo until 02 April 2019. Contaminants find their way to the Arctic through long-range atmospheric transport, transport via ocean currents, and through increased anthropogenic activity. Some of the typical pollutants reaching the Arctic (PAHs, PCBs) are known to induce cytochrome P450 1a (CYP1A) protein expression and ethoxyresorufin-O-deethylase (EROD) activity through the aryl hydrocarbon receptor (AhR). In addition, some endocrine disrupting chemicals (EDCs) such as estrogen mimics (xenoestrogens) have been documented in Arctic areas and they may interfere with natural sexual development and reproduction. In vitro assays that are capable of detecting effects of such pollutants, covering multiple endpoints, are generally based on mammalian or temperate species and there are currently no well-characterized cell-based in vitro assays for effect assessment from Arctic fish species. The present study aimed to develop a high-throughput and multi-endpoint in vitro assay from Arctic char (Salvelinus alpinus) to provide a non-animal (alternative) testing method for an ecologically relevant Arctic species. A method for isolation and exposure of primary hepatocytes from Arctic char for studying the toxic effects and mode of action (MoA) of pollutants was applied and validated. The multi-versatility of the bioassay was assessed by classical biomarker responses such as cell viability (membrane integrity and metabolic activity), phase I detoxification (CYP1A protein expression, EROD activity) and estrogen receptor (ER) mediated vitellogenin (Vtg) protein expression using a selection of model compounds, environmental pollutants and an environmental extract containing a complex mixture of pollutants. Primary hepatocytes from Arctic char were successfully isolated and culture conditions optimized to identify the most optimal assay conditions for covering multiple endpoints. The hepatocytes responded with concentration-dependent responses to all of the model compounds, most of the environmental pollutants and the ... Article in Journal/Newspaper Arctic Salvelinus alpinus Norwegian Institute for Water research: NIVA Open Access Archive (Brage) Arctic Moa ENVELOPE(15.184,15.184,67.286,67.286) Aquatic Toxicology 187 141 152 |
institution |
Open Polar |
collection |
Norwegian Institute for Water research: NIVA Open Access Archive (Brage) |
op_collection_id |
ftnorskinstvf |
language |
English |
description |
Embargo until 02 April 2019. Contaminants find their way to the Arctic through long-range atmospheric transport, transport via ocean currents, and through increased anthropogenic activity. Some of the typical pollutants reaching the Arctic (PAHs, PCBs) are known to induce cytochrome P450 1a (CYP1A) protein expression and ethoxyresorufin-O-deethylase (EROD) activity through the aryl hydrocarbon receptor (AhR). In addition, some endocrine disrupting chemicals (EDCs) such as estrogen mimics (xenoestrogens) have been documented in Arctic areas and they may interfere with natural sexual development and reproduction. In vitro assays that are capable of detecting effects of such pollutants, covering multiple endpoints, are generally based on mammalian or temperate species and there are currently no well-characterized cell-based in vitro assays for effect assessment from Arctic fish species. The present study aimed to develop a high-throughput and multi-endpoint in vitro assay from Arctic char (Salvelinus alpinus) to provide a non-animal (alternative) testing method for an ecologically relevant Arctic species. A method for isolation and exposure of primary hepatocytes from Arctic char for studying the toxic effects and mode of action (MoA) of pollutants was applied and validated. The multi-versatility of the bioassay was assessed by classical biomarker responses such as cell viability (membrane integrity and metabolic activity), phase I detoxification (CYP1A protein expression, EROD activity) and estrogen receptor (ER) mediated vitellogenin (Vtg) protein expression using a selection of model compounds, environmental pollutants and an environmental extract containing a complex mixture of pollutants. Primary hepatocytes from Arctic char were successfully isolated and culture conditions optimized to identify the most optimal assay conditions for covering multiple endpoints. The hepatocytes responded with concentration-dependent responses to all of the model compounds, most of the environmental pollutants and the ... |
format |
Article in Journal/Newspaper |
author |
Petersen, Karina Hultman, Maria T Tollefsen, Knut Erik |
spellingShingle |
Petersen, Karina Hultman, Maria T Tollefsen, Knut Erik Primary hepatocytes from Arctic char (Salvelinus alpinus) as a relevant Arctic in vitro model for screening contaminants and environmental extracts |
author_facet |
Petersen, Karina Hultman, Maria T Tollefsen, Knut Erik |
author_sort |
Petersen, Karina |
title |
Primary hepatocytes from Arctic char (Salvelinus alpinus) as a relevant Arctic in vitro model for screening contaminants and environmental extracts |
title_short |
Primary hepatocytes from Arctic char (Salvelinus alpinus) as a relevant Arctic in vitro model for screening contaminants and environmental extracts |
title_full |
Primary hepatocytes from Arctic char (Salvelinus alpinus) as a relevant Arctic in vitro model for screening contaminants and environmental extracts |
title_fullStr |
Primary hepatocytes from Arctic char (Salvelinus alpinus) as a relevant Arctic in vitro model for screening contaminants and environmental extracts |
title_full_unstemmed |
Primary hepatocytes from Arctic char (Salvelinus alpinus) as a relevant Arctic in vitro model for screening contaminants and environmental extracts |
title_sort |
primary hepatocytes from arctic char (salvelinus alpinus) as a relevant arctic in vitro model for screening contaminants and environmental extracts |
publisher |
Elsevier |
publishDate |
2017 |
url |
http://hdl.handle.net/11250/2561365 https://doi.org/10.1016/j.aquatox.2017.03.023 |
long_lat |
ENVELOPE(15.184,15.184,67.286,67.286) |
geographic |
Arctic Moa |
geographic_facet |
Arctic Moa |
genre |
Arctic Salvelinus alpinus |
genre_facet |
Arctic Salvelinus alpinus |
op_source |
141-152 187 Aquatic Toxicology |
op_relation |
Norges forskningsråd: 221373 Norges forskningsråd: 196318 Aquatic Toxicology. 2017, 187, 141-152. urn:issn:0166-445X http://hdl.handle.net/11250/2561365 https://doi.org/10.1016/j.aquatox.2017.03.023 cristin:1484870 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.aquatox.2017.03.023 |
container_title |
Aquatic Toxicology |
container_volume |
187 |
container_start_page |
141 |
op_container_end_page |
152 |
_version_ |
1766306746297483264 |