Insights from shell proteome : biomineralization control and environmental adaptation in bivalves
In this study, the SMPs from four commercially important and divergent bivalve species crassostrea gigas (pacific oyster), Mya truncata (soft shell clam), Mytilus edulis (blue mussel) and Pecten maximus (king scallop) were extracted and analysed using standardized extraction protocol and proteomic p...
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Language: | English |
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2017
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Online Access: | https://theses.hal.science/tel-02279108 https://theses.hal.science/tel-02279108/document https://theses.hal.science/tel-02279108/file/VA_ARIVALAGAN_Jaison_04092017.pdf |
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ftnormandieuniv:oai:HAL:tel-02279108v1 2024-06-23T07:52:19+00:00 Insights from shell proteome : biomineralization control and environmental adaptation in bivalves Apport de l’étude du protéome à la compréhension du contrôle de la biominéralisation et de la réponse adaptative de la coquille de mollusques aux modifications environnementales Arivalagan Immanuel, Jaison Rathina Raj Molécules de Communication et Adaptation des Micro-organismes (MCAM) Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Museum national d'histoire naturelle - MNHN PARIS Arul Marie Sophie Berland 2017-09-04 https://theses.hal.science/tel-02279108 https://theses.hal.science/tel-02279108/document https://theses.hal.science/tel-02279108/file/VA_ARIVALAGAN_Jaison_04092017.pdf en eng HAL CCSD NNT: 2017MNHN0026 tel-02279108 https://theses.hal.science/tel-02279108 https://theses.hal.science/tel-02279108/document https://theses.hal.science/tel-02279108/file/VA_ARIVALAGAN_Jaison_04092017.pdf info:eu-repo/semantics/OpenAccess https://theses.hal.science/tel-02279108 Animal biology. Museum national d'histoire naturelle - MNHN PARIS, 2017. English. ⟨NNT : 2017MNHN0026⟩ Biomineralization Proteomics Functional ecology Phenotypic plasticity Adaptation Mollusk Biominéralisation Protéomique Ecologie fonctionnelle Plasticité phénotypique Mollusques [SDV.BA]Life Sciences [q-bio]/Animal biology info:eu-repo/semantics/doctoralThesis Theses 2017 ftnormandieuniv 2024-06-11T00:01:58Z In this study, the SMPs from four commercially important and divergent bivalve species crassostrea gigas (pacific oyster), Mya truncata (soft shell clam), Mytilus edulis (blue mussel) and Pecten maximus (king scallop) were extracted and analysed using standardized extraction protocol and proteomic pipeline. This enables us to identify critical elements of basic biomineralization tool kit for calcification process irrespective of their shell morphology, mineralogy and microstructure. In addition, it enables the identification of SMPs that are specific to calcite and aragonite mineralogies. The signifiant numbers of SMPs found species-specific were hypothesized as adaptation to their modus vivendi. In fact, the latter proteins possess immunity-related functions and fit into specific pathway, phenoloxidase, suggesting their role in defense against pathogen. The comparative study of shell proteome of mussels living in full marine condition, North Sea and the Iow saline Baltic Sea showed the modulation of the SMPs that constitute the basic biomineralization tool kit. Higher modulation of chitin related proteins and non-modulated protein such as carbonic anhydrase, EGF and fibronectin domain containing proteins points out the impaired scaffold and mineral nucleation process in Baltic mussel. The modulation of immunity related proteins denote the influence of biotic components. These investigations show the functional diversity of SMPs and their roles beyond shell formation in the bivalvesand put forth the idea that shell is dynamic, endowed with both biochemical and mechanical protection. Le processus de biominéralisation confère aux organismes qui le développent une valeur adaptative. La coquille carbonatée des mollusques intègre les fonctions de protection biomécanique à différentes échelles. La coquille résulte de l'association de composés inorganiques et d'une matrice organique protéique, médiatrice du contrôle biologique de la minéralisation. L'analyse du protéome de la coquille chez 4 espèces de bivalves met ... Doctoral or Postdoctoral Thesis Crassostrea gigas Pacific oyster Normandie Université: HAL Pacific |
institution |
Open Polar |
collection |
Normandie Université: HAL |
op_collection_id |
ftnormandieuniv |
language |
English |
topic |
Biomineralization Proteomics Functional ecology Phenotypic plasticity Adaptation Mollusk Biominéralisation Protéomique Ecologie fonctionnelle Plasticité phénotypique Mollusques [SDV.BA]Life Sciences [q-bio]/Animal biology |
spellingShingle |
Biomineralization Proteomics Functional ecology Phenotypic plasticity Adaptation Mollusk Biominéralisation Protéomique Ecologie fonctionnelle Plasticité phénotypique Mollusques [SDV.BA]Life Sciences [q-bio]/Animal biology Arivalagan Immanuel, Jaison Rathina Raj Insights from shell proteome : biomineralization control and environmental adaptation in bivalves |
topic_facet |
Biomineralization Proteomics Functional ecology Phenotypic plasticity Adaptation Mollusk Biominéralisation Protéomique Ecologie fonctionnelle Plasticité phénotypique Mollusques [SDV.BA]Life Sciences [q-bio]/Animal biology |
description |
In this study, the SMPs from four commercially important and divergent bivalve species crassostrea gigas (pacific oyster), Mya truncata (soft shell clam), Mytilus edulis (blue mussel) and Pecten maximus (king scallop) were extracted and analysed using standardized extraction protocol and proteomic pipeline. This enables us to identify critical elements of basic biomineralization tool kit for calcification process irrespective of their shell morphology, mineralogy and microstructure. In addition, it enables the identification of SMPs that are specific to calcite and aragonite mineralogies. The signifiant numbers of SMPs found species-specific were hypothesized as adaptation to their modus vivendi. In fact, the latter proteins possess immunity-related functions and fit into specific pathway, phenoloxidase, suggesting their role in defense against pathogen. The comparative study of shell proteome of mussels living in full marine condition, North Sea and the Iow saline Baltic Sea showed the modulation of the SMPs that constitute the basic biomineralization tool kit. Higher modulation of chitin related proteins and non-modulated protein such as carbonic anhydrase, EGF and fibronectin domain containing proteins points out the impaired scaffold and mineral nucleation process in Baltic mussel. The modulation of immunity related proteins denote the influence of biotic components. These investigations show the functional diversity of SMPs and their roles beyond shell formation in the bivalvesand put forth the idea that shell is dynamic, endowed with both biochemical and mechanical protection. Le processus de biominéralisation confère aux organismes qui le développent une valeur adaptative. La coquille carbonatée des mollusques intègre les fonctions de protection biomécanique à différentes échelles. La coquille résulte de l'association de composés inorganiques et d'une matrice organique protéique, médiatrice du contrôle biologique de la minéralisation. L'analyse du protéome de la coquille chez 4 espèces de bivalves met ... |
author2 |
Molécules de Communication et Adaptation des Micro-organismes (MCAM) Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Museum national d'histoire naturelle - MNHN PARIS Arul Marie Sophie Berland |
format |
Doctoral or Postdoctoral Thesis |
author |
Arivalagan Immanuel, Jaison Rathina Raj |
author_facet |
Arivalagan Immanuel, Jaison Rathina Raj |
author_sort |
Arivalagan Immanuel, Jaison Rathina Raj |
title |
Insights from shell proteome : biomineralization control and environmental adaptation in bivalves |
title_short |
Insights from shell proteome : biomineralization control and environmental adaptation in bivalves |
title_full |
Insights from shell proteome : biomineralization control and environmental adaptation in bivalves |
title_fullStr |
Insights from shell proteome : biomineralization control and environmental adaptation in bivalves |
title_full_unstemmed |
Insights from shell proteome : biomineralization control and environmental adaptation in bivalves |
title_sort |
insights from shell proteome : biomineralization control and environmental adaptation in bivalves |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://theses.hal.science/tel-02279108 https://theses.hal.science/tel-02279108/document https://theses.hal.science/tel-02279108/file/VA_ARIVALAGAN_Jaison_04092017.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
https://theses.hal.science/tel-02279108 Animal biology. Museum national d'histoire naturelle - MNHN PARIS, 2017. English. ⟨NNT : 2017MNHN0026⟩ |
op_relation |
NNT: 2017MNHN0026 tel-02279108 https://theses.hal.science/tel-02279108 https://theses.hal.science/tel-02279108/document https://theses.hal.science/tel-02279108/file/VA_ARIVALAGAN_Jaison_04092017.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1802643579389083648 |