Gonadotropin-inhibitory hormone in teleosts: New insights from a basal representative, the eel

• Published in: General and Comparative Endocrinology
• Main Authors: Maugars, G., Pasquier, J., Atkinson, C., Lafont, Anne-Gaelle, Campo, A., Kamech, N., Lefranc, B., Leprince, J., Dufour, S., Rousseau, K.
• Other Authors: Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Différenciation et communication neuronale et neuroendocrine (DC2N), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2020
• Subjects: Anguilla; GnIH; GnRH-R; Gonadotropin subunits; NPVF; Phylogeny; Pituitary cell culture; Pituitary hormones; Synteny; Tissue distribution; MESH: Animals; MESH: Eels; MESH: Female; MESH: Gonadotropin-Releasing Hormone; MESH: Phylogeny; MESH: Pituitary Gland; MESH: Synteny; [SDV]Life Sciences [q-bio]; Anguilla anguilla; European eel
• Online Access: https://hal.science/hal-02989422; https://hal.science/hal-02989422/document; https://hal.science/hal-02989422/file/S0016648019303296.pdf; https://doi.org/10.1016/j.ygcen.2019.113350

International audience Since its discovery in birds, gonadotropin-inhibitory hormone (GnIH) has triggered investigation in the other groups of vertebrates. In the present study, we have identified a single gnih gene in the European eel (Anguilla anguilla), a representative species of a basal group of teleosts (Elopomorphs). We have also retrieved a single gnih gene in Osteoglossomorphs, as well as in more recently emerged teleosts, Clupeocephala. Phylogeny and synteny analyses allowed us to infer that one of the two gnih paralogs emerged from the teleost-specific whole genome duplication (TWGD or 3R), would have been lost shortly after the 3R, before the emergence of the basal groups of teleosts. This led to the presence of a single gnih in extant teleosts as in other vertebrates. Two gnih paralogs were still found in some teleost species, such as in salmonids, but resulting from the additional whole genome duplication that specifically occurred in this lineage (4R). Eel gnih was mostly expressed in the diencephalon part of the brain, as analyzed by quantitative real-time PCR. Cloning of eel gnih cDNA confirmed that the sequence of the GnIH precursor encoded three putative mature GnIH peptides (aaGnIH-1, aaGnIH-2 and aaGnIH-3), which were synthesized and tested for their direct effects on eel pituitary cells in vitro. Eel GnIH peptides inhibited the expression of gonadotropin subunits (lhβ, fshβ, and common a-subunit) as well as of GnRH receptor (gnrh-r2), with no effect on tshβ and gh expression. The inhibitory effect of GnIH peptides on gonadotropic function in a basal teleost is in agreement with an ancestral inhibitory role of GnIH in the neuroendocrine control of reproduction in vertebrates.