Transcriptional expression levels of cell stress marker genes in the pacific oyster Crassostrea gigas exposed to acute thermal stress
International audience Abstract During the annual cycle, oysters are exposed to seasonal slow changes in temperature, but during emersion at low tide on sunny summer days, their internal temperature may rise rapidly, resulting in acute heat stress. We experimentally exposed oysters to a 1-h acute th...
Published in: | Cell Stress and Chaperones |
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Format: | Article in Journal/Newspaper |
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Online Access: | https://hal.science/hal-02988579 https://doi.org/10.1007/s12192-008-0091-8 |
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ftnormandieuniv:oai:HAL:hal-02988579v1 2024-04-28T08:16:36+00:00 Transcriptional expression levels of cell stress marker genes in the pacific oyster Crassostrea gigas exposed to acute thermal stress Farcy, É. Voiseux, C. Lebel, J.-M. Fiévet, B. Laboratoire de Radioécologie de Cherbourg-Octeville (IRSN/PSE-ENV/SRTE/LRC) Service de recherche sur les transferts et les effets des radionucléides sur les écosystèmes (IRSN/PSE-ENV/SRTE) Institut de Radioprotection et de Sûreté Nucléaire (IRSN)-Institut de Radioprotection et de Sûreté Nucléaire (IRSN) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) 2009-07-01 https://hal.science/hal-02988579 https://doi.org/10.1007/s12192-008-0091-8 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.1007/s12192-008-0091-8 hal-02988579 https://hal.science/hal-02988579 doi:10.1007/s12192-008-0091-8 PUBMEDCENTRAL: PMC2728272 Cell Stress and Chaperones https://hal.science/hal-02988579 Cell Stress and Chaperones, 2009, 14 (4), pp.371-380. ⟨10.1007/s12192-008-0091-8⟩ heat stress marker gene nonhuman nucleotide sequence oxidative stress oyster priority journal quantitative analysis reverse transcription polymerase chain reaction seasonal variation summer temperature stress winter animal gene expression profiling genetics heat shock response metabolism Pacific Ocean season temperature Crassostrea gigas Ostreidae Animals Biological Markers Crassostrea Heat-Shock Response RNA Messenger Seasons actin biological marker copper zinc superoxide dismutase cytochrome P450 glutathione transferase heat shock protein 70 heat shock protein 72 heat shock protein 90 messenger RNA metallothionein multidrug resistance protein 1 protein p53 article cell cycle regulation cell stress controlled study ebb tide environmental temperature info:eu-repo/semantics/article Journal articles 2009 ftnormandieuniv https://doi.org/10.1007/s12192-008-0091-8 2024-04-11T00:35:07Z International audience Abstract During the annual cycle, oysters are exposed to seasonal slow changes in temperature, but during emersion at low tide on sunny summer days, their internal temperature may rise rapidly, resulting in acute heat stress. We experimentally exposed oysters to a 1-h acute thermal stress and investigated the transcriptional expression level of some genes involved in cell stress defence mechanisms, including chaperone proteins (heat shock proteins Hsp70, Hsp72 and Hsp90 (HSP)), regulation of oxidative stress (Cu-Zn superoxide dismutase, metallothionein (MT)), cell detoxification (glutathione 5-transferase sigma, cytochrome P450 and multidrug resistance (MDR1)) and regulation of the cell cycle (p53). Gene mRNA levels were quantified by reverse transcription-quantitative polymerase chain reaction and expressed as their ratio to actin mRNA, used as a reference. Of the nine genes studied, HSP, MT and MDR1 mRNA levels increased in response to thermal stress. We compared the responses of oysters exposed to acute heat shock in summer and winter and observed differences in terms of magnitude and kinetics. A larger increase was observed in September, with recovery within 48 h, whereas in March, the increase was smaller and lasted more than 2 days. The results were also compared with data obtained from the natural environment. Though the functional molecule is the protein and information at the mRNA level only has limitations, the potential use of mRNAs coding for cell stress defence proteins as early sensitive biomarkers is discussed. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Normandie Université: HAL Cell Stress and Chaperones 14 4 371 380 |
institution |
Open Polar |
collection |
Normandie Université: HAL |
op_collection_id |
ftnormandieuniv |
language |
English |
topic |
heat stress marker gene nonhuman nucleotide sequence oxidative stress oyster priority journal quantitative analysis reverse transcription polymerase chain reaction seasonal variation summer temperature stress winter animal gene expression profiling genetics heat shock response metabolism Pacific Ocean season temperature Crassostrea gigas Ostreidae Animals Biological Markers Crassostrea Heat-Shock Response RNA Messenger Seasons actin biological marker copper zinc superoxide dismutase cytochrome P450 glutathione transferase heat shock protein 70 heat shock protein 72 heat shock protein 90 messenger RNA metallothionein multidrug resistance protein 1 protein p53 article cell cycle regulation cell stress controlled study ebb tide environmental temperature |
spellingShingle |
heat stress marker gene nonhuman nucleotide sequence oxidative stress oyster priority journal quantitative analysis reverse transcription polymerase chain reaction seasonal variation summer temperature stress winter animal gene expression profiling genetics heat shock response metabolism Pacific Ocean season temperature Crassostrea gigas Ostreidae Animals Biological Markers Crassostrea Heat-Shock Response RNA Messenger Seasons actin biological marker copper zinc superoxide dismutase cytochrome P450 glutathione transferase heat shock protein 70 heat shock protein 72 heat shock protein 90 messenger RNA metallothionein multidrug resistance protein 1 protein p53 article cell cycle regulation cell stress controlled study ebb tide environmental temperature Farcy, É. Voiseux, C. Lebel, J.-M. Fiévet, B. Transcriptional expression levels of cell stress marker genes in the pacific oyster Crassostrea gigas exposed to acute thermal stress |
topic_facet |
heat stress marker gene nonhuman nucleotide sequence oxidative stress oyster priority journal quantitative analysis reverse transcription polymerase chain reaction seasonal variation summer temperature stress winter animal gene expression profiling genetics heat shock response metabolism Pacific Ocean season temperature Crassostrea gigas Ostreidae Animals Biological Markers Crassostrea Heat-Shock Response RNA Messenger Seasons actin biological marker copper zinc superoxide dismutase cytochrome P450 glutathione transferase heat shock protein 70 heat shock protein 72 heat shock protein 90 messenger RNA metallothionein multidrug resistance protein 1 protein p53 article cell cycle regulation cell stress controlled study ebb tide environmental temperature |
description |
International audience Abstract During the annual cycle, oysters are exposed to seasonal slow changes in temperature, but during emersion at low tide on sunny summer days, their internal temperature may rise rapidly, resulting in acute heat stress. We experimentally exposed oysters to a 1-h acute thermal stress and investigated the transcriptional expression level of some genes involved in cell stress defence mechanisms, including chaperone proteins (heat shock proteins Hsp70, Hsp72 and Hsp90 (HSP)), regulation of oxidative stress (Cu-Zn superoxide dismutase, metallothionein (MT)), cell detoxification (glutathione 5-transferase sigma, cytochrome P450 and multidrug resistance (MDR1)) and regulation of the cell cycle (p53). Gene mRNA levels were quantified by reverse transcription-quantitative polymerase chain reaction and expressed as their ratio to actin mRNA, used as a reference. Of the nine genes studied, HSP, MT and MDR1 mRNA levels increased in response to thermal stress. We compared the responses of oysters exposed to acute heat shock in summer and winter and observed differences in terms of magnitude and kinetics. A larger increase was observed in September, with recovery within 48 h, whereas in March, the increase was smaller and lasted more than 2 days. The results were also compared with data obtained from the natural environment. Though the functional molecule is the protein and information at the mRNA level only has limitations, the potential use of mRNAs coding for cell stress defence proteins as early sensitive biomarkers is discussed. |
author2 |
Laboratoire de Radioécologie de Cherbourg-Octeville (IRSN/PSE-ENV/SRTE/LRC) Service de recherche sur les transferts et les effets des radionucléides sur les écosystèmes (IRSN/PSE-ENV/SRTE) Institut de Radioprotection et de Sûreté Nucléaire (IRSN)-Institut de Radioprotection et de Sûreté Nucléaire (IRSN) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Farcy, É. Voiseux, C. Lebel, J.-M. Fiévet, B. |
author_facet |
Farcy, É. Voiseux, C. Lebel, J.-M. Fiévet, B. |
author_sort |
Farcy, É. |
title |
Transcriptional expression levels of cell stress marker genes in the pacific oyster Crassostrea gigas exposed to acute thermal stress |
title_short |
Transcriptional expression levels of cell stress marker genes in the pacific oyster Crassostrea gigas exposed to acute thermal stress |
title_full |
Transcriptional expression levels of cell stress marker genes in the pacific oyster Crassostrea gigas exposed to acute thermal stress |
title_fullStr |
Transcriptional expression levels of cell stress marker genes in the pacific oyster Crassostrea gigas exposed to acute thermal stress |
title_full_unstemmed |
Transcriptional expression levels of cell stress marker genes in the pacific oyster Crassostrea gigas exposed to acute thermal stress |
title_sort |
transcriptional expression levels of cell stress marker genes in the pacific oyster crassostrea gigas exposed to acute thermal stress |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://hal.science/hal-02988579 https://doi.org/10.1007/s12192-008-0091-8 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Cell Stress and Chaperones https://hal.science/hal-02988579 Cell Stress and Chaperones, 2009, 14 (4), pp.371-380. ⟨10.1007/s12192-008-0091-8⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12192-008-0091-8 hal-02988579 https://hal.science/hal-02988579 doi:10.1007/s12192-008-0091-8 PUBMEDCENTRAL: PMC2728272 |
op_doi |
https://doi.org/10.1007/s12192-008-0091-8 |
container_title |
Cell Stress and Chaperones |
container_volume |
14 |
container_issue |
4 |
container_start_page |
371 |
op_container_end_page |
380 |
_version_ |
1797581659918303232 |