Isolation and characterization of novel glycoproteins from fish epidermal mucus : correlation between their pore-forming properties and their antibacterial activities
International audience In fish, a layer of mucus covers the external body surface contributing therefore, among other important biological functions, to the defense system of fish. The prevention of colonization by aquatic parasites, bacteria and fungi is mediated both by immune system compounds (Ig...
Published in: | Biochimica et Biophysica Acta (BBA) - Biomembranes |
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Online Access: | https://hal.inrae.fr/hal-02697262 https://doi.org/10.1016/S0005-2736(00)00225-X |
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ftnormandieuniv:oai:HAL:hal-02697262v1 2023-05-15T13:27:56+02:00 Isolation and characterization of novel glycoproteins from fish epidermal mucus : correlation between their pore-forming properties and their antibacterial activities Ebran, Nathalie Julien, Sylviane Orange, Nicole Aupérin, Benoît Molle, Gérard Université de Rouen Normandie (UNIROUEN) Normandie Université (NU) IUT d'Evreux Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE) Institut National de la Recherche Agronomique (INRA) 2000 https://hal.inrae.fr/hal-02697262 https://doi.org/10.1016/S0005-2736(00)00225-X en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/S0005-2736(00)00225-X info:eu-repo/semantics/altIdentifier/pmid/11030587 hal-02697262 https://hal.inrae.fr/hal-02697262 doi:10.1016/S0005-2736(00)00225-X PRODINRA: 55700 PUBMED: 11030587 WOS: 000089222700002 ISSN: 0006-3002 BBA - Biochimica et Biophysica Acta https://hal.inrae.fr/hal-02697262 BBA - Biochimica et Biophysica Acta, Elsevier, 2000, 1467, pp.271-280. ⟨10.1016/S0005-2736(00)00225-X⟩ ANGUILLIDAE ANGUILLA CYPRINIDAE TINCA TINCA SALMONIDAE POISSON [SDV]Life Sciences [q-bio] [INFO]Computer Science [cs] info:eu-repo/semantics/article Journal articles 2000 ftnormandieuniv https://doi.org/10.1016/S0005-2736(00)00225-X 2022-04-26T22:39:00Z International audience In fish, a layer of mucus covers the external body surface contributing therefore, among other important biological functions, to the defense system of fish. The prevention of colonization by aquatic parasites, bacteria and fungi is mediated both by immune system compounds (IgM, lysozyme, etc.) and by antibacterial peptides and polypeptides. We have recently shown that only the hydrophobic components of crude epidermal mucus of fresh water and sea water fish exhibit strong pore-forming properties, which were well correlated with antibacterial activity [N. Ebran, S. Julien, N. Orange, P. Saglio, C. Lemaitre, G. Molle, Comp. Biochem. Physiol. 122 (1999)]. Here, we have isolated novel glycosylated proteins from the hydrophobic supernatant of tench (Tinca tinca), eel (Anguilla anguilla) and rainbow trout (Oncorhynchus mykiss) mucus. The study of their secondary structure was performed by circular dichroism and revealed structures in random coil and α-helix in the same proportions. When reconstituted in planar lipid bilayer, they induced the formation of ion channels. This pore-forming activity was well correlated with a strong antibacterial activity (minimal inhibitory concentration<1 μM for the three proteins) against both Gram-negative and Gram-positive bacteria. Our results suggest that fish secrete antibacterial glycoproteins able to kill bacteria by forming large pores (several hundreds to thousands of pS) in the target membrane. Article in Journal/Newspaper Anguilla anguilla Normandie Université: HAL Biochimica et Biophysica Acta (BBA) - Biomembranes 1467 2 271 280 |
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Normandie Université: HAL |
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ftnormandieuniv |
language |
English |
topic |
ANGUILLIDAE ANGUILLA CYPRINIDAE TINCA TINCA SALMONIDAE POISSON [SDV]Life Sciences [q-bio] [INFO]Computer Science [cs] |
spellingShingle |
ANGUILLIDAE ANGUILLA CYPRINIDAE TINCA TINCA SALMONIDAE POISSON [SDV]Life Sciences [q-bio] [INFO]Computer Science [cs] Ebran, Nathalie Julien, Sylviane Orange, Nicole Aupérin, Benoît Molle, Gérard Isolation and characterization of novel glycoproteins from fish epidermal mucus : correlation between their pore-forming properties and their antibacterial activities |
topic_facet |
ANGUILLIDAE ANGUILLA CYPRINIDAE TINCA TINCA SALMONIDAE POISSON [SDV]Life Sciences [q-bio] [INFO]Computer Science [cs] |
description |
International audience In fish, a layer of mucus covers the external body surface contributing therefore, among other important biological functions, to the defense system of fish. The prevention of colonization by aquatic parasites, bacteria and fungi is mediated both by immune system compounds (IgM, lysozyme, etc.) and by antibacterial peptides and polypeptides. We have recently shown that only the hydrophobic components of crude epidermal mucus of fresh water and sea water fish exhibit strong pore-forming properties, which were well correlated with antibacterial activity [N. Ebran, S. Julien, N. Orange, P. Saglio, C. Lemaitre, G. Molle, Comp. Biochem. Physiol. 122 (1999)]. Here, we have isolated novel glycosylated proteins from the hydrophobic supernatant of tench (Tinca tinca), eel (Anguilla anguilla) and rainbow trout (Oncorhynchus mykiss) mucus. The study of their secondary structure was performed by circular dichroism and revealed structures in random coil and α-helix in the same proportions. When reconstituted in planar lipid bilayer, they induced the formation of ion channels. This pore-forming activity was well correlated with a strong antibacterial activity (minimal inhibitory concentration<1 μM for the three proteins) against both Gram-negative and Gram-positive bacteria. Our results suggest that fish secrete antibacterial glycoproteins able to kill bacteria by forming large pores (several hundreds to thousands of pS) in the target membrane. |
author2 |
Université de Rouen Normandie (UNIROUEN) Normandie Université (NU) IUT d'Evreux Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE) Institut National de la Recherche Agronomique (INRA) |
format |
Article in Journal/Newspaper |
author |
Ebran, Nathalie Julien, Sylviane Orange, Nicole Aupérin, Benoît Molle, Gérard |
author_facet |
Ebran, Nathalie Julien, Sylviane Orange, Nicole Aupérin, Benoît Molle, Gérard |
author_sort |
Ebran, Nathalie |
title |
Isolation and characterization of novel glycoproteins from fish epidermal mucus : correlation between their pore-forming properties and their antibacterial activities |
title_short |
Isolation and characterization of novel glycoproteins from fish epidermal mucus : correlation between their pore-forming properties and their antibacterial activities |
title_full |
Isolation and characterization of novel glycoproteins from fish epidermal mucus : correlation between their pore-forming properties and their antibacterial activities |
title_fullStr |
Isolation and characterization of novel glycoproteins from fish epidermal mucus : correlation between their pore-forming properties and their antibacterial activities |
title_full_unstemmed |
Isolation and characterization of novel glycoproteins from fish epidermal mucus : correlation between their pore-forming properties and their antibacterial activities |
title_sort |
isolation and characterization of novel glycoproteins from fish epidermal mucus : correlation between their pore-forming properties and their antibacterial activities |
publisher |
HAL CCSD |
publishDate |
2000 |
url |
https://hal.inrae.fr/hal-02697262 https://doi.org/10.1016/S0005-2736(00)00225-X |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
ISSN: 0006-3002 BBA - Biochimica et Biophysica Acta https://hal.inrae.fr/hal-02697262 BBA - Biochimica et Biophysica Acta, Elsevier, 2000, 1467, pp.271-280. ⟨10.1016/S0005-2736(00)00225-X⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0005-2736(00)00225-X info:eu-repo/semantics/altIdentifier/pmid/11030587 hal-02697262 https://hal.inrae.fr/hal-02697262 doi:10.1016/S0005-2736(00)00225-X PRODINRA: 55700 PUBMED: 11030587 WOS: 000089222700002 |
op_doi |
https://doi.org/10.1016/S0005-2736(00)00225-X |
container_title |
Biochimica et Biophysica Acta (BBA) - Biomembranes |
container_volume |
1467 |
container_issue |
2 |
container_start_page |
271 |
op_container_end_page |
280 |
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1766401247045222400 |