Effect of salinity and temperature on the expression of genes involved in branchial ion transport processes in European sea bass

• Published in: Journal of Thermal Biology
• Main Authors: Masroor, Waliullah, Farcy, Emilie, Blondeau-Bidet, Eva, Venn, Alexander, Tambutte, Eric, Lorin-Nebel, Catherine
• Other Authors: MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Centre Scientifique de Monaco (CSM), Université de Montpellier (UM)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2019
• Subjects: dicentrarchus-labrax; fresh-water; Dicentrarchus labrax; Salinity; cftr anion channel; cod gadus-morhua; Gill; h+-atpase; chloride channel; functional classification; Ion transporter; mRNA expression; na+/k+-atpase; na+/k+/2cl(-) cotransporter; seawater-acclimated medaka; Thermal acclimation; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; [SDV.EE.ECO]Life Sciences [q-bio]/Ecology; environment/Ecosystems; Gadus morhua
• Online Access: https://hal.science/hal-02439695; https://hal.science/hal-02439695/document; https://hal.science/hal-02439695/file/S0306456519301950.pdf; https://doi.org/10.1016/j.jtherbio.2019.102422

WOS:000497246100023 International audience The responses of European sea bass to temperature increase and salinity decrease were investigated measuring mRNA expression levels of main genes involved in ion transport. Juvenile fish were pre-acclimated to seawater (SW) at 18 °C (temperate) or 24 °C (warm) for two weeks and then transferred for two weeks to either fresh water (FW) or SW at the respective temperature. Unlike temperate conditions, there is no change in Na+/K+-ATPase α1a (nka α1a) and Na+/H+ exchanger 3 (nhe3) mRNA expression following FW transfer in warm conditions. This is linked to the high expression of these genes in warm SW compared to temperate SW. Na+/Cl−-cotransporter (ncc2a) expression however is increased following FW transfer in temperate and warm conditions. Main transporters involved in ion excretion (Na+/K+/2Cl--1 cotransporter, nkcc1 and cystic fibrosis transmembrane conductance regulator, cftr) as well as nitrogen excretion (Rh-glycoproteins, rhcg1 and rhbg) and acid-base regulation (V-H+-ATPase, vha-a and b) are highly expressed in SW warm conditions vs FW warm. Overall, our results suggest a higher activation of ion transport processes in warm conditions and more strikingly in SW. This is linked to a strong interplay between diverse ion transporters in order to coordinate physiological responses at the gill level.