Oyster sex determination is influenced by temperature — First clues in spat during first gonadic differentiation and gametogenesis
International audience The sex-determining system of Crassostrea gigas is still poorly known, especially regarding the potential influence of temperature. In order to address this question, mRNA expressions of actors of the molecular cascade (Cg-DMl, Cg-SoxE, Cg-β-catenin, Cg-Foxl2/Cg-Foxl2os) and o...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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ftnormandieuniv:oai:HAL:hal-02296506v1 2023-11-12T04:16:15+01:00 Oyster sex determination is influenced by temperature — First clues in spat during first gonadic differentiation and gametogenesis Santerre, Christelle Sourdaine, Pascal Marc, Nicolas Mingant, Christian Robert, René Martinez, Anne-Sophie Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2013-05 https://normandie-univ.hal.science/hal-02296506 https://doi.org/10.1016/j.cbpa.2013.02.007 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2013.02.007 hal-02296506 https://normandie-univ.hal.science/hal-02296506 doi:10.1016/j.cbpa.2013.02.007 ISSN: 1095-6433 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology https://normandie-univ.hal.science/hal-02296506 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, 2013, 165 (1), pp.61-69. ⟨10.1016/j.cbpa.2013.02.007⟩ Oyster spat Temperature Sex ratio TSD Sex determination pathway [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2013 ftnormandieuniv https://doi.org/10.1016/j.cbpa.2013.02.007 2023-10-17T22:47:49Z International audience The sex-determining system of Crassostrea gigas is still poorly known, especially regarding the potential influence of temperature. In order to address this question, mRNA expressions of actors of the molecular cascade (Cg-DMl, Cg-SoxE, Cg-β-catenin, Cg-Foxl2/Cg-Foxl2os) and of Oyvlg, a germ cell marker, were investigated by real-time PCR in spat grown at different temperatures (18, 22, 25 and 28 °C). In parallel, gonadic differentiation, gametogenesis and sex ratios were assessed by histology at each of these temperatures. Whatever the temperature, Cg-DMl, Cg-SoxE, Cg-β-catenin and Oyvlg expressions peaked at the same developmental stage, always after Cg-Foxl2/Cg-Foxl2os (around 40–44 dpf for spat grown at 18 °C). Temperatures increased the kinetics of first gonadic differentiation and gametogenesis. At 25 °C a significant switch occurred in sex ratio towards males and in the balance of expression between male and female genes, in favor of males. A slight gametogenesis disturbance was also observed. These results strengthen the hypotheses about the sex-determining time window and molecular cascade governing the development of C. gigas, with notably the involvement of Cg-Foxl2/Cg-Foxl2os in the very early steps. They also suggest an influence of temperature on the oyster's sex determination which, associated to genetic control, would induce a mixed sex determination system (GSD + TSD). Article in Journal/Newspaper Crassostrea gigas DML Normandie Université: HAL Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 165 1 61 69 |
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Normandie Université: HAL |
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ftnormandieuniv |
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English |
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Oyster spat Temperature Sex ratio TSD Sex determination pathway [SDV]Life Sciences [q-bio] |
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Oyster spat Temperature Sex ratio TSD Sex determination pathway [SDV]Life Sciences [q-bio] Santerre, Christelle Sourdaine, Pascal Marc, Nicolas Mingant, Christian Robert, René Martinez, Anne-Sophie Oyster sex determination is influenced by temperature — First clues in spat during first gonadic differentiation and gametogenesis |
topic_facet |
Oyster spat Temperature Sex ratio TSD Sex determination pathway [SDV]Life Sciences [q-bio] |
description |
International audience The sex-determining system of Crassostrea gigas is still poorly known, especially regarding the potential influence of temperature. In order to address this question, mRNA expressions of actors of the molecular cascade (Cg-DMl, Cg-SoxE, Cg-β-catenin, Cg-Foxl2/Cg-Foxl2os) and of Oyvlg, a germ cell marker, were investigated by real-time PCR in spat grown at different temperatures (18, 22, 25 and 28 °C). In parallel, gonadic differentiation, gametogenesis and sex ratios were assessed by histology at each of these temperatures. Whatever the temperature, Cg-DMl, Cg-SoxE, Cg-β-catenin and Oyvlg expressions peaked at the same developmental stage, always after Cg-Foxl2/Cg-Foxl2os (around 40–44 dpf for spat grown at 18 °C). Temperatures increased the kinetics of first gonadic differentiation and gametogenesis. At 25 °C a significant switch occurred in sex ratio towards males and in the balance of expression between male and female genes, in favor of males. A slight gametogenesis disturbance was also observed. These results strengthen the hypotheses about the sex-determining time window and molecular cascade governing the development of C. gigas, with notably the involvement of Cg-Foxl2/Cg-Foxl2os in the very early steps. They also suggest an influence of temperature on the oyster's sex determination which, associated to genetic control, would induce a mixed sex determination system (GSD + TSD). |
author2 |
Biologie des mollusques marins et des écosystèmes associés (BioMEA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Santerre, Christelle Sourdaine, Pascal Marc, Nicolas Mingant, Christian Robert, René Martinez, Anne-Sophie |
author_facet |
Santerre, Christelle Sourdaine, Pascal Marc, Nicolas Mingant, Christian Robert, René Martinez, Anne-Sophie |
author_sort |
Santerre, Christelle |
title |
Oyster sex determination is influenced by temperature — First clues in spat during first gonadic differentiation and gametogenesis |
title_short |
Oyster sex determination is influenced by temperature — First clues in spat during first gonadic differentiation and gametogenesis |
title_full |
Oyster sex determination is influenced by temperature — First clues in spat during first gonadic differentiation and gametogenesis |
title_fullStr |
Oyster sex determination is influenced by temperature — First clues in spat during first gonadic differentiation and gametogenesis |
title_full_unstemmed |
Oyster sex determination is influenced by temperature — First clues in spat during first gonadic differentiation and gametogenesis |
title_sort |
oyster sex determination is influenced by temperature — first clues in spat during first gonadic differentiation and gametogenesis |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://normandie-univ.hal.science/hal-02296506 https://doi.org/10.1016/j.cbpa.2013.02.007 |
genre |
Crassostrea gigas DML |
genre_facet |
Crassostrea gigas DML |
op_source |
ISSN: 1095-6433 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology https://normandie-univ.hal.science/hal-02296506 Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, 2013, 165 (1), pp.61-69. ⟨10.1016/j.cbpa.2013.02.007⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2013.02.007 hal-02296506 https://normandie-univ.hal.science/hal-02296506 doi:10.1016/j.cbpa.2013.02.007 |
op_doi |
https://doi.org/10.1016/j.cbpa.2013.02.007 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
165 |
container_issue |
1 |
container_start_page |
61 |
op_container_end_page |
69 |
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1782333395465928704 |